Assembly of osteoblastic cell micro-arrays on diamond guided by protein pre-adsorption. Rezek, B., Ukraintsev, E., Kromka, A., Ledinský, M., Broz, A., Nosková, L., Hartmannová, H., & Kalbacova, M. Diamond and Related Materials, 19(2-3):153--157, 2010. Paper doi abstract bibtex We investigate the role of fetal bovine serum (FBS) proteins in the assembly of osteoblastic cells (SAOS-2) in McCoy's 5A medium on synthetic monocrystalline and nanocrystalline diamonds having H- and O-terminated array patterns. We characterize i) adhesion of proteins and cells to diamond by shaking experiments (1000 rpm), fluorescence, and atomic force microscopy, ii) distribution of FBS proteins by micro-Raman spectroscopy, and iii) influence of pre-adsorbed specific FBS proteins (albumin, fibronectin, vitronectin) on the cell assembly. There is 40% lower adhesion of cells on H-terminated diamond with adsorbed FBS compared to this surface without FBS as well as to O-terminated surfaces. Spatially resolved Raman spectroscopy of C--H stretching bands around 2900 cm- 1 showed uniform coverage of H/O-diamond by the albumin in more than 50 nm thick FBS layers. The other FBS proteins were not detectable by Raman. Pre-adsorption of the specific proteins prior to cell plating indicated that the cell preference to O-terminated diamond is controlled by the fibronectin rather than by the albumin. We discuss the data with a view to the cell preferential assembly on H/O-diamond micro-arrays.
@article{ rezek_assembly_2010,
title = {Assembly of osteoblastic cell micro-arrays on diamond guided by protein pre-adsorption},
volume = {19},
issn = {0925-9635},
url = {http://www.sciencedirect.com/science/article/B6TWV-4XCYJC2-2/2/81cc1101403891024a201470e04b61e5},
doi = {10.1016/j.diamond.2009.09.016},
abstract = {We investigate the role of fetal bovine serum ({FBS}) proteins in the assembly of osteoblastic cells ({SAOS}-2) in {McCoy}'s 5A medium on synthetic monocrystalline and nanocrystalline diamonds having H- and O-terminated array patterns. We characterize i) adhesion of proteins and cells to diamond by shaking experiments (1000 rpm), fluorescence, and atomic force microscopy, ii) distribution of {FBS} proteins by micro-Raman spectroscopy, and iii) influence of pre-adsorbed specific {FBS} proteins (albumin, fibronectin, vitronectin) on the cell assembly. There is 40% lower adhesion of cells on H-terminated diamond with adsorbed {FBS} compared to this surface without {FBS} as well as to O-terminated surfaces. Spatially resolved Raman spectroscopy of C--H stretching bands around 2900 cm- 1 showed uniform coverage of H/O-diamond by the albumin in more than 50 nm thick {FBS} layers. The other {FBS} proteins were not detectable by Raman. Pre-adsorption of the specific proteins prior to cell plating indicated that the cell preference to O-terminated diamond is controlled by the fibronectin rather than by the albumin. We discuss the data with a view to the cell preferential assembly on H/O-diamond micro-arrays.},
number = {2-3},
urldate = {2011-02-07TZ},
journal = {Diamond and Related Materials},
author = {Rezek, B. and Ukraintsev, E. and Kromka, A. and Ledinský, M. and Broz, A. and Nosková, L. and Hartmannová, H. and Kalbacova, M.},
year = {2010},
keywords = {Biomaterials, Cell adhesion, Diamond, Raman spectroscopy, atomic force microscopy, proteins},
pages = {153--157}
}
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We characterize i) adhesion of proteins and cells to diamond by shaking experiments (1000 rpm), fluorescence, and atomic force microscopy, ii) distribution of {FBS} proteins by micro-Raman spectroscopy, and iii) influence of pre-adsorbed specific {FBS} proteins (albumin, fibronectin, vitronectin) on the cell assembly. There is 40% lower adhesion of cells on H-terminated diamond with adsorbed {FBS} compared to this surface without {FBS} as well as to O-terminated surfaces. Spatially resolved Raman spectroscopy of C--H stretching bands around 2900 cm- 1 showed uniform coverage of H/O-diamond by the albumin in more than 50 nm thick {FBS} layers. The other {FBS} proteins were not detectable by Raman. Pre-adsorption of the specific proteins prior to cell plating indicated that the cell preference to O-terminated diamond is controlled by the fibronectin rather than by the albumin. 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