@article{ rezek_assembly_2010,
  title = {Assembly of osteoblastic cell micro-arrays on diamond guided by protein pre-adsorption},
  volume = {19},
  issn = {0925-9635},
  url = {http://www.sciencedirect.com/science/article/B6TWV-4XCYJC2-2/2/81cc1101403891024a201470e04b61e5},
  doi = {10.1016/j.diamond.2009.09.016},
  abstract = {We investigate the role of fetal bovine serum ({FBS}) proteins in the assembly of osteoblastic cells ({SAOS}-2) in {McCoy}'s 5A medium on synthetic monocrystalline and nanocrystalline diamonds having H- and O-terminated array patterns. We characterize i) adhesion of proteins and cells to diamond by shaking experiments (1000 rpm), fluorescence, and atomic force microscopy, ii) distribution of {FBS} proteins by micro-Raman spectroscopy, and iii) influence of pre-adsorbed specific {FBS} proteins (albumin, fibronectin, vitronectin) on the cell assembly. There is 40% lower adhesion of cells on H-terminated diamond with adsorbed {FBS} compared to this surface without {FBS} as well as to O-terminated surfaces. Spatially resolved Raman spectroscopy of C--H stretching bands around 2900 cm- 1 showed uniform coverage of H/O-diamond by the albumin in more than 50 nm thick {FBS} layers. The other {FBS} proteins were not detectable by Raman. Pre-adsorption of the specific proteins prior to cell plating indicated that the cell preference to O-terminated diamond is controlled by the fibronectin rather than by the albumin. We discuss the data with a view to the cell preferential assembly on H/O-diamond micro-arrays.},
  number = {2-3},
  urldate = {2011-02-07TZ},
  journal = {Diamond and Related Materials},
  author = {Rezek, B. and Ukraintsev, E. and Kromka, A. and Ledinský, M. and Broz, A. and Nosková, L. and Hartmannová, H. and Kalbacova, M.},
  year = {2010},
  keywords = {Biomaterials, Cell adhesion, Diamond, Raman spectroscopy, atomic force microscopy, proteins},
  pages = {153--157}
}

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We characterize i) adhesion of proteins and cells to diamond by shaking experiments (1000 rpm), fluorescence, and atomic force microscopy, ii) distribution of FBS proteins by micro-Raman spectroscopy, and iii) influence of pre-adsorbed specific FBS proteins (albumin, fibronectin, vitronectin) on the cell assembly. There is 40% lower adhesion of cells on H-terminated diamond with adsorbed FBS compared to this surface without FBS as well as to O-terminated surfaces. Spatially resolved Raman spectroscopy of C--H stretching bands around 2900 cm- 1 showed uniform coverage of H/O-diamond by the albumin in more than 50 nm thick FBS layers. The other FBS proteins were not detectable by Raman. Pre-adsorption of the specific proteins prior to cell plating indicated that the cell preference to O-terminated diamond is controlled by the fibronectin rather than by the albumin. We discuss the data with a view to the cell preferential assembly on H/O-diamond micro-arrays.","author":["Rezek, B.","Ukraintsev, E.","Kromka, A.","Ledinský, M.","Broz, A.","Nosková, L.","Hartmannová, H.","Kalbacova, M."],"author_short":["Rezek, B.","Ukraintsev, E.","Kromka, A.","Ledinský, M.","Broz, A.","Nosková, L.","Hartmannová, H.","Kalbacova, M."],"bibtex":"@article{ rezek_assembly_2010,\n title = {Assembly of osteoblastic cell micro-arrays on diamond guided by protein pre-adsorption},\n volume = {19},\n issn = {0925-9635},\n url = {http://www.sciencedirect.com/science/article/B6TWV-4XCYJC2-2/2/81cc1101403891024a201470e04b61e5},\n doi = {10.1016/j.diamond.2009.09.016},\n abstract = {We investigate the role of fetal bovine serum ({FBS}) proteins in the assembly of osteoblastic cells ({SAOS}-2) in {McCoy}'s 5A medium on synthetic monocrystalline and nanocrystalline diamonds having H- and O-terminated array patterns. We characterize i) adhesion of proteins and cells to diamond by shaking experiments (1000 rpm), fluorescence, and atomic force microscopy, ii) distribution of {FBS} proteins by micro-Raman spectroscopy, and iii) influence of pre-adsorbed specific {FBS} proteins (albumin, fibronectin, vitronectin) on the cell assembly. There is 40% lower adhesion of cells on H-terminated diamond with adsorbed {FBS} compared to this surface without {FBS} as well as to O-terminated surfaces. Spatially resolved Raman spectroscopy of C--H stretching bands around 2900 cm- 1 showed uniform coverage of H/O-diamond by the albumin in more than 50 nm thick {FBS} layers. The other {FBS} proteins were not detectable by Raman. Pre-adsorption of the specific proteins prior to cell plating indicated that the cell preference to O-terminated diamond is controlled by the fibronectin rather than by the albumin. We discuss the data with a view to the cell preferential assembly on H/O-diamond micro-arrays.},\n number = {2-3},\n urldate = {2011-02-07TZ},\n journal = {Diamond and Related Materials},\n author = {Rezek, B. and Ukraintsev, E. and Kromka, A. and Ledinský, M. and Broz, A. and Nosková, L. and Hartmannová, H. and Kalbacova, M.},\n year = {2010},\n keywords = {Biomaterials, Cell adhesion, Diamond, Raman spectroscopy, atomic force microscopy, proteins},\n pages = {153--157}\n}","bibtype":"article","doi":"10.1016/j.diamond.2009.09.016","id":"rezek_assembly_2010","issn":"0925-9635","journal":"Diamond and Related Materials","key":"rezek_assembly_2010","keywords":"Biomaterials, Cell adhesion, Diamond, Raman spectroscopy, atomic force microscopy, proteins","number":"2-3","pages":"153--157","title":"Assembly of osteoblastic cell micro-arrays on diamond guided by protein pre-adsorption","type":"article","url":"http://www.sciencedirect.com/science/article/B6TWV-4XCYJC2-2/2/81cc1101403891024a201470e04b61e5","urldate":"2011-02-07TZ","volume":"19","year":"2010","bibbaseid":"rezek-ukraintsev-kromka-ledinsk-broz-noskov-hartmannov-kalbacova-assemblyofosteoblasticcellmicroarraysondiamondguidedbyproteinpreadsorption-2010","role":"author","urls":{"Paper":"http://www.sciencedirect.com/science/article/B6TWV-4XCYJC2-2/2/81cc1101403891024a201470e04b61e5"},"keyword":["Biomaterials","Cell adhesion","Diamond","Raman spectroscopy","atomic force microscopy","proteins"],"downloads":0},"search_terms":["assembly","osteoblastic","cell","micro","arrays","diamond","guided","protein","pre","adsorption","rezek","ukraintsev","kromka","ledinský","broz","nosková","hartmannová","kalbacova"],"keywords":["biomaterials","cell adhesion","diamond","raman spectroscopy","atomic force microscopy","proteins"],"authorIDs":[],"dataSources":["WPnZAo8YwWpk4So4c"]}