Setting-up and validation of a respiratory distress adult rabbit model managed in nasal continuous positive airway pressure ventilation. Ricci F., Catozzi C., Murgia X., Lorenzin L., Amidani D., Rosa B., Bianco F., Pioselli B., Dani C., & Salomone F. 2016. abstract bibtex Background and aims Nasal continuous positive airway pressure (nCPAP) is a widely accepted technique of non-invasive respiratory support in spontaneously-breathing premature infants with respiratory distress syndrome (RDS) due to lack of lung surfactant. Surfactant administration technique compatible with nCPAP ventilation strategy are actively investigated. The aim of this study is therefore to set up and validate an animal model of respiratory distress that can be managed on nCPAP for the evaluation of surfactant administration techniques study. Methods Surfactant depletion was induced by bronchoalveolar lavage (BAL) on 18 adult rabbits. Full depletion was assessed by surfactant component analysis on the BAL samples. Animals were randomized into two groups: no treatment (nCPAP only group) and InSurE Curosurf(200mg/kg; Chiesi Farmaceutici S.p.A., Parma, Italy) treatment. Arterial blood gases were monitored until animal sacrifice, 3 hours post treatment. Lung mechanics were evaluated before BAL, before treatment and before sacrifice. Results Surfactant phospholipids and protein analysis on sequential BALs confirmed the efficacy of the surfactant depletion procedure. The InSurE treatment group showed significant blood oxygenation and lung mechanics improvements. On the contrary, no signs of recovery were observed in the nCPAP only group. Conclusions The surfactant-depleted adult rabbit respiratory distress model proved to be a valuable and efficient preclinical tool for mimicking the clinical scenario of preterm infants affected by mild/moderate RDS who spontaneously breathe and do not require mechanical ventilation. This population might be of particular interest as potential target for the non-invasive administration of surfactants. (Figure Presented).
@misc{ricci_f._setting-up_2016,
title = {Setting-up and validation of a respiratory distress adult rabbit model managed in nasal continuous positive airway pressure ventilation},
abstract = {Background and aims Nasal continuous positive airway pressure (nCPAP) is a widely accepted technique of non-invasive respiratory support in spontaneously-breathing premature infants with respiratory distress syndrome (RDS) due to lack of lung surfactant. Surfactant administration technique compatible with nCPAP ventilation strategy are actively investigated. The aim of this study is therefore to set up and validate an animal model of respiratory distress that can be managed on nCPAP for the evaluation of surfactant administration techniques study. Methods Surfactant depletion was induced by bronchoalveolar lavage (BAL) on 18 adult rabbits. Full depletion was assessed by surfactant component analysis on the BAL samples. Animals were randomized into two groups: no treatment (nCPAP only group) and InSurE Curosurf(200mg/kg; Chiesi Farmaceutici S.p.A., Parma, Italy) treatment. Arterial blood gases were monitored until animal sacrifice, 3 hours post treatment. Lung mechanics were evaluated before BAL, before treatment and before sacrifice. Results Surfactant phospholipids and protein analysis on sequential BALs confirmed the efficacy of the surfactant depletion procedure. The InSurE treatment group showed significant blood oxygenation and lung mechanics improvements. On the contrary, no signs of recovery were observed in the nCPAP only group. Conclusions The surfactant-depleted adult rabbit respiratory distress model proved to be a valuable and efficient preclinical tool for mimicking the clinical scenario of preterm infants affected by mild/moderate RDS who spontaneously breathe and do not require mechanical ventilation. This population might be of particular interest as potential target for the non-invasive administration of surfactants. (Figure Presented).},
journal = {European Journal of Pediatrics},
author = {{Ricci F.} and {Catozzi C.} and {Murgia X.} and {Lorenzin L.} and {Amidani D.} and {Rosa B.} and {Bianco F.} and {Pioselli B.} and {Dani C.} and {Salomone F.}},
year = {2016},
keywords = {*positive end expiratory pressure, *rabbit model, *respiratory distress syndrome, *validation process, Italy, adult, animal experiment, animal model, arterial blood, blood gas, blood oxygenation, controlled study, disease model, endogenous compound, infant, lavage fluid, lung lavage, lung mechanics, nonhuman, phospholipid, preclinical study, prematurity, protein analysis, surfactant}
}
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{"_id":"AGqdZub63Cs54eR4S","bibbaseid":"riccif-catozzic-murgiax-lorenzinl-amidanid-rosab-biancof-piosellib-etal-settingupandvalidationofarespiratorydistressadultrabbitmodelmanagedinnasalcontinuouspositiveairwaypressureventilation-2016","downloads":0,"creationDate":"2017-05-12T20:20:57.516Z","title":"Setting-up and validation of a respiratory distress adult rabbit model managed in nasal continuous positive airway pressure ventilation","author_short":["Ricci F.","Catozzi C.","Murgia X.","Lorenzin L.","Amidani D.","Rosa B.","Bianco F.","Pioselli B.","Dani C.","Salomone F."],"year":2016,"bibtype":"misc","biburl":"http://bibbase.org/zotero/gxu917","bibdata":{"bibtype":"misc","type":"misc","title":"Setting-up and validation of a respiratory distress adult rabbit model managed in nasal continuous positive airway pressure ventilation","abstract":"Background and aims Nasal continuous positive airway pressure (nCPAP) is a widely accepted technique of non-invasive respiratory support in spontaneously-breathing premature infants with respiratory distress syndrome (RDS) due to lack of lung surfactant. Surfactant administration technique compatible with nCPAP ventilation strategy are actively investigated. The aim of this study is therefore to set up and validate an animal model of respiratory distress that can be managed on nCPAP for the evaluation of surfactant administration techniques study. Methods Surfactant depletion was induced by bronchoalveolar lavage (BAL) on 18 adult rabbits. Full depletion was assessed by surfactant component analysis on the BAL samples. Animals were randomized into two groups: no treatment (nCPAP only group) and InSurE Curosurf(200mg/kg; Chiesi Farmaceutici S.p.A., Parma, Italy) treatment. Arterial blood gases were monitored until animal sacrifice, 3 hours post treatment. Lung mechanics were evaluated before BAL, before treatment and before sacrifice. Results Surfactant phospholipids and protein analysis on sequential BALs confirmed the efficacy of the surfactant depletion procedure. The InSurE treatment group showed significant blood oxygenation and lung mechanics improvements. On the contrary, no signs of recovery were observed in the nCPAP only group. Conclusions The surfactant-depleted adult rabbit respiratory distress model proved to be a valuable and efficient preclinical tool for mimicking the clinical scenario of preterm infants affected by mild/moderate RDS who spontaneously breathe and do not require mechanical ventilation. This population might be of particular interest as potential target for the non-invasive administration of surfactants. (Figure Presented).","journal":"European Journal of Pediatrics","author":[{"firstnames":[],"propositions":[],"lastnames":["Ricci F."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Catozzi C."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Murgia X."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Lorenzin L."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Amidani D."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Rosa B."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Bianco F."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Pioselli B."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Dani C."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Salomone F."],"suffixes":[]}],"year":"2016","keywords":"*positive end expiratory pressure, *rabbit model, *respiratory distress syndrome, *validation process, Italy, adult, animal experiment, animal model, arterial blood, blood gas, blood oxygenation, controlled study, disease model, endogenous compound, infant, lavage fluid, lung lavage, lung mechanics, nonhuman, phospholipid, preclinical study, prematurity, protein analysis, surfactant","bibtex":"@misc{ricci_f._setting-up_2016,\n\ttitle = {Setting-up and validation of a respiratory distress adult rabbit model managed in nasal continuous positive airway pressure ventilation},\n\tabstract = {Background and aims Nasal continuous positive airway pressure (nCPAP) is a widely accepted technique of non-invasive respiratory support in spontaneously-breathing premature infants with respiratory distress syndrome (RDS) due to lack of lung surfactant. Surfactant administration technique compatible with nCPAP ventilation strategy are actively investigated. The aim of this study is therefore to set up and validate an animal model of respiratory distress that can be managed on nCPAP for the evaluation of surfactant administration techniques study. Methods Surfactant depletion was induced by bronchoalveolar lavage (BAL) on 18 adult rabbits. Full depletion was assessed by surfactant component analysis on the BAL samples. Animals were randomized into two groups: no treatment (nCPAP only group) and InSurE Curosurf(200mg/kg; Chiesi Farmaceutici S.p.A., Parma, Italy) treatment. Arterial blood gases were monitored until animal sacrifice, 3 hours post treatment. Lung mechanics were evaluated before BAL, before treatment and before sacrifice. Results Surfactant phospholipids and protein analysis on sequential BALs confirmed the efficacy of the surfactant depletion procedure. The InSurE treatment group showed significant blood oxygenation and lung mechanics improvements. On the contrary, no signs of recovery were observed in the nCPAP only group. Conclusions The surfactant-depleted adult rabbit respiratory distress model proved to be a valuable and efficient preclinical tool for mimicking the clinical scenario of preterm infants affected by mild/moderate RDS who spontaneously breathe and do not require mechanical ventilation. This population might be of particular interest as potential target for the non-invasive administration of surfactants. (Figure Presented).},\n\tjournal = {European Journal of Pediatrics},\n\tauthor = {{Ricci F.} and {Catozzi C.} and {Murgia X.} and {Lorenzin L.} and {Amidani D.} and {Rosa B.} and {Bianco F.} and {Pioselli B.} and {Dani C.} and {Salomone F.}},\n\tyear = {2016},\n\tkeywords = {*positive end expiratory pressure, *rabbit model, *respiratory distress syndrome, *validation process, Italy, adult, animal experiment, animal model, arterial blood, blood gas, blood oxygenation, controlled study, disease model, endogenous compound, infant, lavage fluid, lung lavage, lung mechanics, nonhuman, phospholipid, preclinical study, prematurity, protein analysis, surfactant}\n}\n\n","author_short":["Ricci F.","Catozzi C.","Murgia X.","Lorenzin L.","Amidani D.","Rosa B.","Bianco F.","Pioselli B.","Dani C.","Salomone F."],"key":"ricci_f._setting-up_2016","id":"ricci_f._setting-up_2016","bibbaseid":"riccif-catozzic-murgiax-lorenzinl-amidanid-rosab-biancof-piosellib-etal-settingupandvalidationofarespiratorydistressadultrabbitmodelmanagedinnasalcontinuouspositiveairwaypressureventilation-2016","role":"author","urls":{},"keyword":["*positive end expiratory pressure","*rabbit model","*respiratory distress syndrome","*validation process","Italy","adult","animal experiment","animal model","arterial blood","blood gas","blood oxygenation","controlled study","disease model","endogenous compound","infant","lavage fluid","lung lavage","lung mechanics","nonhuman","phospholipid","preclinical study","prematurity","protein analysis","surfactant"],"downloads":0,"html":""},"search_terms":["setting","validation","respiratory","distress","adult","rabbit","model","managed","nasal","continuous","positive","airway","pressure","ventilation","ricci f.","catozzi c.","murgia x.","lorenzin l.","amidani d.","rosa b.","bianco f.","pioselli b.","dani c.","salomone f."],"keywords":["*positive end expiratory pressure","*rabbit model","*respiratory distress syndrome","*validation process","italy","adult","animal experiment","animal model","arterial blood","blood gas","blood oxygenation","controlled study","disease model","endogenous compound","infant","lavage fluid","lung lavage","lung mechanics","nonhuman","phospholipid","preclinical study","prematurity","protein analysis","surfactant"],"authorIDs":[],"dataSources":["9LqPNaySNpNxYBFzj"]}