@misc{ricci_f._physiological_2017,
	title = {Physiological, biochemical, and biophysical characterization of the lung-lavaged spontaneously-breathing rabbit as a model for respiratory distress syndrome},
	url = {http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0169190&type=printable},
	abstract = {Nasal continuous positive airway pressure (nCPAP) is a widely accepted technique of noninvasive respiratory support in spontaneously-breathing premature infants with respiratory distress syndrome (RDS). Surfactant administration techniques compatible with nCPAP ventilation strategy are actively investigated. Our aim is to set up and validate a respiratory distress animal model that can be managed on nCPAP suitable for surfactant administration techniques studies. Surfactant depletion was induced by bronchoalveolar lavages (BALs) on 18 adult rabbits. Full depletion was assessed by surfactant component analysis on the BALs samples. Animals were randomized into two groups: Control group (nCPAP only) and InSurE group, consisting of a bolus of surfactant (Poractant alfa, 200 mg/kg) followed by nCPAP. Arterial blood gases were monitored until animal sacrifice, 3 hours post treatment. Lung mechanics were evaluated just before and after BALs, at the time of treatment, and at the end of the procedure. Surfactant phospholipids and protein analysis as well as surface tension measurements on sequential BALs confirmed the efficacy of the surfactant depletion procedure. The InSurE group showed a significant improvement of blood oxygenation and lung mechanics. On the contrary, no signs of recovery were appreciated in animals treated with just nCPAP. The surfactant-depleted adult rabbit RDS model proved to be a valuable and efficient preclinical tool for mimicking the clinical scenario of preterm infants affected by mild/moderate RDS who spontaneously breathe and do not require mechanical ventilation. This population is of particular interest as potential target for the non-invasive administration of surfactant. Copyright © 2017 Ricci et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},
	journal = {PLoS ONE},
	author = {{Ricci F.} and {Catozzi C.} and {Murgia X.} and {Rosa B.} and {Amidani D.} and {Lorenzini L.} and {Bianco F.} and {Rivetti C.} and {Catinella S.} and {Villetti G.} and {Civelli M.} and {Pioselli B.} and {Dani C.} and {Salomone F.}},
	year = {2017},
	keywords = {*artificial ventilation, *disease model, *lung lavage, *physical model, *positive end expiratory pressure, *rabbit, *respiratory distress syndrome, *respiratory distress syndrome/dt [Drug Therapy], *respiratory distress syndrome/th [Therapy], adult, airway pressure, analysis of variance, animal experiment, animal model, arterial blood, article, blood gas, blood gas analysis, blood oxygenation, control group, controlled study, disease severity, endogenous compound, gas exchange, infant, liquid chromatography, lung mechanics, mass spectrometry, nonhuman, outcome assessment, phospholipid, poractant, poractant/dt [Drug Therapy], positive end expiratory pressure, preclinical study, prematurity, protein analysis, qualitative analysis, quantitative analysis, respiratory distress syndrome/dt [Drug Therapy], respiratory failure, surface tension, surfactant, surfactant protein B/ec [Endogenous Compound], surfactant protein C/ec [Endogenous Compound]}
}

{"_id":"s37HkhGvDf9M8vmyC","bibbaseid":"riccif-catozzic-murgiax-rosab-amidanid-lorenzinil-biancof-rivettic-etal-physiologicalbiochemicalandbiophysicalcharacterizationofthelunglavagedspontaneouslybreathingrabbitasamodelforrespiratorydistresssyndrome-2017","downloads":0,"creationDate":"2017-05-12T20:20:57.871Z","title":"Physiological, biochemical, and biophysical characterization of the lung-lavaged spontaneously-breathing rabbit as a model for respiratory distress syndrome","author_short":["Ricci F.","Catozzi C.","Murgia X.","Rosa B.","Amidani D.","Lorenzini L.","Bianco F.","Rivetti C.","Catinella S.","Villetti G.","Civelli M.","Pioselli B.","Dani C.","Salomone F."],"year":2017,"bibtype":"misc","biburl":"http://bibbase.org/zotero/gxu917","bibdata":{"bibtype":"misc","type":"misc","title":"Physiological, biochemical, and biophysical characterization of the lung-lavaged spontaneously-breathing rabbit as a model for respiratory distress syndrome","url":"http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0169190&type=printable","abstract":"Nasal continuous positive airway pressure (nCPAP) is a widely accepted technique of noninvasive respiratory support in spontaneously-breathing premature infants with respiratory distress syndrome (RDS). Surfactant administration techniques compatible with nCPAP ventilation strategy are actively investigated. Our aim is to set up and validate a respiratory distress animal model that can be managed on nCPAP suitable for surfactant administration techniques studies. Surfactant depletion was induced by bronchoalveolar lavages (BALs) on 18 adult rabbits. Full depletion was assessed by surfactant component analysis on the BALs samples. Animals were randomized into two groups: Control group (nCPAP only) and InSurE group, consisting of a bolus of surfactant (Poractant alfa, 200 mg/kg) followed by nCPAP. Arterial blood gases were monitored until animal sacrifice, 3 hours post treatment. Lung mechanics were evaluated just before and after BALs, at the time of treatment, and at the end of the procedure. Surfactant phospholipids and protein analysis as well as surface tension measurements on sequential BALs confirmed the efficacy of the surfactant depletion procedure. The InSurE group showed a significant improvement of blood oxygenation and lung mechanics. On the contrary, no signs of recovery were appreciated in animals treated with just nCPAP. The surfactant-depleted adult rabbit RDS model proved to be a valuable and efficient preclinical tool for mimicking the clinical scenario of preterm infants affected by mild/moderate RDS who spontaneously breathe and do not require mechanical ventilation. This population is of particular interest as potential target for the non-invasive administration of surfactant. Copyright © 2017 Ricci et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.","journal":"PLoS ONE","author":[{"firstnames":[],"propositions":[],"lastnames":["Ricci F."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Catozzi C."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Murgia X."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Rosa B."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Amidani D."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Lorenzini L."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Bianco F."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Rivetti C."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Catinella S."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Villetti G."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Civelli M."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Pioselli B."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Dani C."],"suffixes":[]},{"firstnames":[],"propositions":[],"lastnames":["Salomone F."],"suffixes":[]}],"year":"2017","keywords":"*artificial ventilation, *disease model, *lung lavage, *physical model, *positive end expiratory pressure, *rabbit, *respiratory distress syndrome, *respiratory distress syndrome/dt [Drug Therapy], *respiratory distress syndrome/th [Therapy], adult, airway pressure, analysis of variance, animal experiment, animal model, arterial blood, article, blood gas, blood gas analysis, blood oxygenation, control group, controlled study, disease severity, endogenous compound, gas exchange, infant, liquid chromatography, lung mechanics, mass spectrometry, nonhuman, outcome assessment, phospholipid, poractant, poractant/dt [Drug Therapy], positive end expiratory pressure, preclinical study, prematurity, protein analysis, qualitative analysis, quantitative analysis, respiratory distress syndrome/dt [Drug Therapy], respiratory failure, surface tension, surfactant, surfactant protein B/ec [Endogenous Compound], surfactant protein C/ec [Endogenous Compound]","bibtex":"@misc{ricci_f._physiological_2017,\n\ttitle = {Physiological, biochemical, and biophysical characterization of the lung-lavaged spontaneously-breathing rabbit as a model for respiratory distress syndrome},\n\turl = {http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0169190&type=printable},\n\tabstract = {Nasal continuous positive airway pressure (nCPAP) is a widely accepted technique of noninvasive respiratory support in spontaneously-breathing premature infants with respiratory distress syndrome (RDS). Surfactant administration techniques compatible with nCPAP ventilation strategy are actively investigated. Our aim is to set up and validate a respiratory distress animal model that can be managed on nCPAP suitable for surfactant administration techniques studies. Surfactant depletion was induced by bronchoalveolar lavages (BALs) on 18 adult rabbits. Full depletion was assessed by surfactant component analysis on the BALs samples. Animals were randomized into two groups: Control group (nCPAP only) and InSurE group, consisting of a bolus of surfactant (Poractant alfa, 200 mg/kg) followed by nCPAP. Arterial blood gases were monitored until animal sacrifice, 3 hours post treatment. Lung mechanics were evaluated just before and after BALs, at the time of treatment, and at the end of the procedure. Surfactant phospholipids and protein analysis as well as surface tension measurements on sequential BALs confirmed the efficacy of the surfactant depletion procedure. The InSurE group showed a significant improvement of blood oxygenation and lung mechanics. On the contrary, no signs of recovery were appreciated in animals treated with just nCPAP. The surfactant-depleted adult rabbit RDS model proved to be a valuable and efficient preclinical tool for mimicking the clinical scenario of preterm infants affected by mild/moderate RDS who spontaneously breathe and do not require mechanical ventilation. This population is of particular interest as potential target for the non-invasive administration of surfactant. Copyright © 2017 Ricci et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.},\n\tjournal = {PLoS ONE},\n\tauthor = {{Ricci F.} and {Catozzi C.} and {Murgia X.} and {Rosa B.} and {Amidani D.} and {Lorenzini L.} and {Bianco F.} and {Rivetti C.} and {Catinella S.} and {Villetti G.} and {Civelli M.} and {Pioselli B.} and {Dani C.} and {Salomone F.}},\n\tyear = {2017},\n\tkeywords = {*artificial ventilation, *disease model, *lung lavage, *physical model, *positive end expiratory pressure, *rabbit, *respiratory distress syndrome, *respiratory distress syndrome/dt [Drug Therapy], *respiratory distress syndrome/th [Therapy], adult, airway pressure, analysis of variance, animal experiment, animal model, arterial blood, article, blood gas, blood gas analysis, blood oxygenation, control group, controlled study, disease severity, endogenous compound, gas exchange, infant, liquid chromatography, lung mechanics, mass spectrometry, nonhuman, outcome assessment, phospholipid, poractant, poractant/dt [Drug Therapy], positive end expiratory pressure, preclinical study, prematurity, protein analysis, qualitative analysis, quantitative analysis, respiratory distress syndrome/dt [Drug Therapy], respiratory failure, surface tension, surfactant, surfactant protein B/ec [Endogenous Compound], surfactant protein C/ec [Endogenous Compound]}\n}\n\n","author_short":["Ricci F.","Catozzi C.","Murgia X.","Rosa B.","Amidani D.","Lorenzini L.","Bianco F.","Rivetti C.","Catinella S.","Villetti G.","Civelli M.","Pioselli B.","Dani C.","Salomone F."],"key":"ricci_f._physiological_2017","id":"ricci_f._physiological_2017","bibbaseid":"riccif-catozzic-murgiax-rosab-amidanid-lorenzinil-biancof-rivettic-etal-physiologicalbiochemicalandbiophysicalcharacterizationofthelunglavagedspontaneouslybreathingrabbitasamodelforrespiratorydistresssyndrome-2017","role":"author","urls":{"Paper":"http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0169190&type=printable"},"keyword":["*artificial ventilation","*disease model","*lung lavage","*physical model","*positive end expiratory pressure","*rabbit","*respiratory distress syndrome","*respiratory distress syndrome/dt [Drug Therapy]","*respiratory distress syndrome/th [Therapy]","adult","airway pressure","analysis of variance","animal experiment","animal model","arterial blood","article","blood gas","blood gas analysis","blood oxygenation","control group","controlled study","disease severity","endogenous compound","gas exchange","infant","liquid chromatography","lung mechanics","mass spectrometry","nonhuman","outcome assessment","phospholipid","poractant","poractant/dt [Drug Therapy]","positive end expiratory pressure","preclinical study","prematurity","protein analysis","qualitative analysis","quantitative analysis","respiratory distress syndrome/dt [Drug Therapy]","respiratory failure","surface tension","surfactant","surfactant protein B/ec [Endogenous Compound]","surfactant protein C/ec [Endogenous Compound]"],"downloads":0,"html":""},"search_terms":["physiological","biochemical","biophysical","characterization","lung","lavaged","spontaneously","breathing","rabbit","model","respiratory","distress","syndrome","ricci f.","catozzi c.","murgia x.","rosa b.","amidani d.","lorenzini l.","bianco f.","rivetti c.","catinella s.","villetti g.","civelli m.","pioselli b.","dani c.","salomone f."],"keywords":["*artificial ventilation","*disease model","*lung lavage","*physical model","*positive end expiratory pressure","*rabbit","*respiratory distress syndrome","*respiratory distress syndrome/dt [drug therapy]","*respiratory distress syndrome/th [therapy]","adult","airway pressure","analysis of variance","animal experiment","animal model","arterial blood","article","blood gas","blood gas analysis","blood oxygenation","control group","controlled study","disease severity","endogenous compound","gas exchange","infant","liquid chromatography","lung mechanics","mass spectrometry","nonhuman","outcome assessment","phospholipid","poractant","poractant/dt [drug therapy]","positive end expiratory pressure","preclinical study","prematurity","protein analysis","qualitative analysis","quantitative analysis","respiratory distress syndrome/dt [drug therapy]","respiratory failure","surface tension","surfactant","surfactant protein b/ec [endogenous compound]","surfactant protein c/ec [endogenous compound]"],"authorIDs":[],"dataSources":["9LqPNaySNpNxYBFzj"]}