Water-in-PDMS emulsion templating of highly interconnected porous architectures for 3D cell culture. Riesco, R., Boyer, L., Blosse, S., Lefebvre, P. M, Assemat, P., Leichlé, T., Accardo, A., & Malaquin, L. ACS applied materials & interfaces, jul, 2019. ![Water-in-PDMS emulsion templating of highly interconnected porous architectures for 3D cell culture. [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex The development of advanced techniques of fabrication of 3D microenvironments for the study of cell growth and proliferation has become one of the major motivations of material scientists and bioengineers in the past decade. Here, we present a novel residue-less 3D structuration technique of polydimethylsiloxane (PDMS) by water-in-PDMS emulsion casting and subsequent curing process in temperature-pressure controlled environment. Scanning electron microscopy (SEM) and X-ray micro-computed tomography (µCT) allowed us to investigate the impact of those parameters on the microarchitecture of the porous structure. We demonstrated that the optimized emulsion casting process gives rise to large scale and highly interconnected network with pore size ranging from 500 um to 1.5 mm that turned out to be nicely adapted to 3D cell culture. Experimental cell culture validations were performed using SaOS-2 (Osteosarcoma) cell lines. Epifluorescence and deep penetration imaging techniques as two-photon confocal microscopy unveiled information about cell morphology and confirmed a homogeneous cell proliferation and spatial distribution in the 3D porous structure within an available volume larger than 1 cm3. These results open alternatives scenarios for the fabrication and integration of porous scaffolds for the development of 3D cell culture platforms.
@article{Riesco2019,
abstract = {The development of advanced techniques of fabrication of 3D microenvironments for the study of cell growth and proliferation has become one of the major motivations of material scientists and bioengineers in the past decade. Here, we present a novel residue-less 3D structuration technique of polydimethylsiloxane (PDMS) by water-in-PDMS emulsion casting and subsequent curing process in temperature-pressure controlled environment. Scanning electron microscopy (SEM) and X-ray micro-computed tomography (µCT) allowed us to investigate the impact of those parameters on the microarchitecture of the porous structure. We demonstrated that the optimized emulsion casting process gives rise to large scale and highly interconnected network with pore size ranging from 500 um to 1.5 mm that turned out to be nicely adapted to 3D cell culture. Experimental cell culture validations were performed using SaOS-2 (Osteosarcoma) cell lines. Epifluorescence and deep penetration imaging techniques as two-photon confocal microscopy unveiled information about cell morphology and confirmed a homogeneous cell proliferation and spatial distribution in the 3D porous structure within an available volume larger than 1 cm3. These results open alternatives scenarios for the fabrication and integration of porous scaffolds for the development of 3D cell culture platforms.},
author = {Riesco, Roberto and Boyer, Louisa and Blosse, Sarah and Lefebvre, Pauline M and Assemat, Pauline and Leichl{\'{e}}, Thierry and Accardo, Angelo and Malaquin, Laurent},
doi = {10.1021/acsami.9b07564},
issn = {1944-8252},
journal = {ACS applied materials {\&} interfaces},
month = {jul},
pmid = {31334634},
title = {{Water-in-PDMS emulsion templating of highly interconnected porous architectures for 3D cell culture.}},
url = {http://www.ncbi.nlm.nih.gov/pubmed/31334634},
year = {2019}
}
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Scanning electron microscopy (SEM) and X-ray micro-computed tomography (µCT) allowed us to investigate the impact of those parameters on the microarchitecture of the porous structure. We demonstrated that the optimized emulsion casting process gives rise to large scale and highly interconnected network with pore size ranging from 500 um to 1.5 mm that turned out to be nicely adapted to 3D cell culture. Experimental cell culture validations were performed using SaOS-2 (Osteosarcoma) cell lines. Epifluorescence and deep penetration imaging techniques as two-photon confocal microscopy unveiled information about cell morphology and confirmed a homogeneous cell proliferation and spatial distribution in the 3D porous structure within an available volume larger than 1 cm3. 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