Mapping of magnesium and of different protein fragments in sea urchin teeth via secondary ion mass spectroscopy. Robach, J., S.; Stock, S., R.; and Veis, a. Journal of structural biology, 155(1):87-95, 7, 2006.
Mapping of magnesium and of different protein fragments in sea urchin teeth via secondary ion mass spectroscopy. [pdf]Paper  Mapping of magnesium and of different protein fragments in sea urchin teeth via secondary ion mass spectroscopy. [link]Website  abstract   bibtex   
Mature portions of sea urchin are comprised of a complex array of reinforcing elements yet are single crystals of high and very high Mg calcite. How a relatively poor structural material (calcite) can produce mechanically competent structures is of great interest. In teeth of the sea urchin Lytechinus variegatus, we recorded high-resolution secondary ion mass spectrometry (SIMS) maps of Mg, Ca ,and specific amino acid fragments of mineral-related proteins including aspartic acid (Asp). SIMS revealed strong colocalization of Asp residues with very high Mg. Demineralized specimens showed serine localization on membranes between crystal elements and reduced Mg and aspartic acid signals, further emphasizing colocalization of very high Mg with ready soluble Asp-rich protein(s). The association of Asp with nonequilibrium, very high magnesium calcite provides insight to the makeup of the macromolecules involved in the growth of two different composition calcites and the fundamental process of biomineralization.
@article{
 title = {Mapping of magnesium and of different protein fragments in sea urchin teeth via secondary ion mass spectroscopy.},
 type = {article},
 year = {2006},
 identifiers = {[object Object]},
 keywords = {Animals,Calcium,Calcium: chemistry,Lytechinus,Lytechinus: anatomy & histology,Magnesium,Magnesium: chemistry,Models, Biological,Osmolar Concentration,Peptide Mapping,Protein Binding,Sea Urchins,Sea Urchins: anatomy & histology,Spectrometry, Mass, Secondary Ion,Spectrometry, Mass, Secondary Ion: methods,Tooth,Tooth: chemistry},
 pages = {87-95},
 volume = {155},
 websites = {http://www.ncbi.nlm.nih.gov/pubmed/16675267},
 month = {7},
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 abstract = {Mature portions of sea urchin are comprised of a complex array of reinforcing elements yet are single crystals of high and very high Mg calcite. How a relatively poor structural material (calcite) can produce mechanically competent structures is of great interest. In teeth of the sea urchin Lytechinus variegatus, we recorded high-resolution secondary ion mass spectrometry (SIMS) maps of Mg, Ca ,and specific amino acid fragments of mineral-related proteins including aspartic acid (Asp). SIMS revealed strong colocalization of Asp residues with very high Mg. Demineralized specimens showed serine localization on membranes between crystal elements and reduced Mg and aspartic acid signals, further emphasizing colocalization of very high Mg with ready soluble Asp-rich protein(s). The association of Asp with nonequilibrium, very high magnesium calcite provides insight to the makeup of the macromolecules involved in the growth of two different composition calcites and the fundamental process of biomineralization.},
 bibtype = {article},
 author = {Robach, J S and Stock, S R and Veis, a},
 journal = {Journal of structural biology},
 number = {1}
}
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