Isolation of intact vacuoles from Arabidopsis rosette leaf

Isolation of intact vacuoles from Arabidopsis rosette leaf–derived protoplasts. Robert, S., Zouhar, J., Carter, C., & Raikhel, N. Nature Protocols, 2(2):259–262, February, 2007. Number: 2 Publisher: Nature Publishing Group

Paper doi abstract bibtex

Vacuoles are very prominent compartments within plant cells, and understanding of their function relies on knowledge of their content. Here, we present a simple vacuole purification protocol that was successfully used for large-scale isolation of vacuoles, free of significant contamination from other endomembrane compartments. This method is based on osmotic and thermal disruption of mesophyl-derived Arabidopsis protoplasts, followed by a density gradient fractionation of the cellular content. The whole procedure, including protoplast isolation, takes approximately 6 h.

@article{robert_isolation_2007,
	title = {Isolation of intact vacuoles from {Arabidopsis} rosette leaf–derived protoplasts},
	volume = {2},
	copyright = {2007 Nature Publishing Group},
	issn = {1750-2799},
	url = {https://www.nature.com/articles/nprot.2007.26},
	doi = {10/c58qb9},
	abstract = {Vacuoles are very prominent compartments within plant cells, and understanding of their function relies on knowledge of their content. Here, we present a simple vacuole purification protocol that was successfully used for large-scale isolation of vacuoles, free of significant contamination from other endomembrane compartments. This method is based on osmotic and thermal disruption of mesophyl-derived Arabidopsis protoplasts, followed by a density gradient fractionation of the cellular content. The whole procedure, including protoplast isolation, takes approximately 6 h.},
	language = {en},
	number = {2},
	urldate = {2021-06-10},
	journal = {Nature Protocols},
	author = {Robert, Stéphanie and Zouhar, Jan and Carter, Clay and Raikhel, Natasha},
	month = feb,
	year = {2007},
	note = {Number: 2
Publisher: Nature Publishing Group},
	pages = {259--262},
}

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