Differential responses in vitroof rice cultivars to Italian lineages of the blast pathogenPyricularia grisea (Cooke) Sacc. 1. Oxidative burst. Rożkowicz, A., Picco, A. M., Rodolfi, M., Pinamonti, S., & Forlani, G. Journal of Plant Physiology, 160(9):1033--1040, 2003. 00000
Differential responses in vitroof rice cultivars to Italian lineages of the blast pathogenPyricularia grisea (Cooke) Sacc. 1. Oxidative burst [link]Paper  doi  abstract   bibtex   
Summary Suspension cultured cells of six rice cultivars differing in their sensitivity to blast were treated with mycelial wall hydrolysates prepared from seven isolates belonging to different Pyricularia grisea lineages. Soon after elicitor addition, rice cells produced significant amounts of superoxide anion, which was rapidly converted into diffusible peroxide. Maximal effects were achieved at 50 mg L−1 elicitor. In all cases, a 7 to 13-fold increase in the basal rate of reactive oxygen species production was found. Neither differential effects among strains nor clear relationships between lineage and the resulting oxidative burst were evident. Interestingly, a good correlation was found between basal (and elicited) levels of peroxide generation and the overall tolerance of rice cultivars to the pathogen. About two days after elicitation, cell death occurred proportional to the amount of hydrogen peroxide released. Peroxide was required to trigger loss of cell viability, but the latter was not due to a direct toxic effect, suggesting the induction of programmed cell death. Results represent the first data aimed to develop in vitro tests for pathogenicity prediction of Italian blast lineages toward rice cultivars.
@article{rozkowicz_differential_2003,
	title = {Differential responses in vitroof rice cultivars to {Italian} lineages of the blast {pathogenPyricularia} grisea ({Cooke}) {Sacc}. 1. {Oxidative} burst},
	volume = {160},
	issn = {0176-1617},
	url = {http://www.sciencedirect.com/science/article/pii/S0176161704704915},
	doi = {10.1078/0176-1617-00941},
	abstract = {Summary
Suspension cultured cells of six rice cultivars differing in their sensitivity to blast were treated with mycelial wall hydrolysates prepared from seven isolates belonging to different Pyricularia grisea lineages. Soon after elicitor addition, rice cells produced significant amounts of superoxide anion, which was rapidly converted into diffusible peroxide. Maximal effects were achieved at 50 mg L−1 elicitor. In all cases, a 7 to 13-fold increase in the basal rate of reactive oxygen species production was found. Neither differential effects among strains nor clear relationships between lineage and the resulting oxidative burst were evident. Interestingly, a good correlation was found between basal (and elicited) levels of peroxide generation and the overall tolerance of rice cultivars to the pathogen. About two days after elicitation, cell death occurred proportional to the amount of hydrogen peroxide released. Peroxide was required to trigger loss of cell viability, but the latter was not due to a direct toxic effect, suggesting the induction of programmed cell death. Results represent the first data aimed to develop in vitro tests for pathogenicity prediction of Italian blast lineages toward rice cultivars.},
	number = {9},
	urldate = {2015-04-09TZ},
	journal = {Journal of Plant Physiology},
	author = {Rożkowicz, Anna and Picco, Anna M. and Rodolfi, Marinella and Pinamonti, Silvano and Forlani, Giuseppe},
	year = {2003},
	note = {00000},
	keywords = {blast pathogen, fungal elicitor, oxidative burst, pilzbefall, reactive oxygen species, rice, Oryza sativa L},
	pages = {1033--1040}
}

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