Stimulation of respiration by Pb2+ in detached leaves and mitochondria of C3 and C4 plants. Romanowska, E., Igamberdiev, A. U., Parys, E., & Gardeström, P. Physiologia Plantarum, 116(2):148–154, 2002. _eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1034/j.1399-3054.2002.1160203.x![Stimulation of respiration by Pb2+ in detached leaves and mitochondria of C3 and C4 plants [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex The exposure of detached leaves of C3 plants (pea, barley) and C4 plant (maize) to 5 mM Pb (NO3)2 for 24 h caused a reduction of their photosynthetic activity by 40–60%, whereas the respiratory rate was stimulated by 20–50%. Mitochondria isolated from Pb2+-treated pea leaves oxidized substrates (glycine, succinate, malate) at higher rates than mitochondria from control leaves. The respiratory control (RCR) and the ADP/O ratio were not affected. Pb2+ caused an increase in ATP content and the ATP/ADP ratio in pea and maize leaves. Rapid fractionation of barley protoplasts incubated at low and high CO2 conditions, indicated that the increased ATP/ADP ratio in Pb2+-treated leaves resulted mainly from the production of mitochondrial ATP. The measurements of membrane potential of mitochondria with a TPP+-sensitive electrode further showed that mitochondria isolated from Pb2+-treated leaves had at least as high membrane potential as mitochondria from control leaves. The activity of NAD-malate dehydrogenase in the protoplasts from barley leaves treated with Pb2+ was 3-fold higher than in protoplasts from control leaves. The activities of photorespiratory enzymes NADH-hydroxypyruvate reductase and glycolate oxidase as well as of NAD-malic enzyme were not affected. The presented data indicate that stimulation of respiration in leaves treated by lead is in a close relationship with activation of malate dehydrogenase and stimulation of the mitochondrial ATP production. Thus, respiration might fulfil a protective role during heavy metal exposure.
@article{romanowska_stimulation_2002,
title = {Stimulation of respiration by {Pb2}+ in detached leaves and mitochondria of {C3} and {C4} plants},
volume = {116},
issn = {1399-3054},
url = {https://onlinelibrary.wiley.com/doi/abs/10.1034/j.1399-3054.2002.1160203.x},
doi = {10/b54t5q},
abstract = {The exposure of detached leaves of C3 plants (pea, barley) and C4 plant (maize) to 5 mM Pb (NO3)2 for 24 h caused a reduction of their photosynthetic activity by 40–60\%, whereas the respiratory rate was stimulated by 20–50\%. Mitochondria isolated from Pb2+-treated pea leaves oxidized substrates (glycine, succinate, malate) at higher rates than mitochondria from control leaves. The respiratory control (RCR) and the ADP/O ratio were not affected. Pb2+ caused an increase in ATP content and the ATP/ADP ratio in pea and maize leaves. Rapid fractionation of barley protoplasts incubated at low and high CO2 conditions, indicated that the increased ATP/ADP ratio in Pb2+-treated leaves resulted mainly from the production of mitochondrial ATP. The measurements of membrane potential of mitochondria with a TPP+-sensitive electrode further showed that mitochondria isolated from Pb2+-treated leaves had at least as high membrane potential as mitochondria from control leaves. The activity of NAD-malate dehydrogenase in the protoplasts from barley leaves treated with Pb2+ was 3-fold higher than in protoplasts from control leaves. The activities of photorespiratory enzymes NADH-hydroxypyruvate reductase and glycolate oxidase as well as of NAD-malic enzyme were not affected. The presented data indicate that stimulation of respiration in leaves treated by lead is in a close relationship with activation of malate dehydrogenase and stimulation of the mitochondrial ATP production. Thus, respiration might fulfil a protective role during heavy metal exposure.},
language = {en},
number = {2},
urldate = {2021-10-19},
journal = {Physiologia Plantarum},
author = {Romanowska, Elżbieta and Igamberdiev, Abir U. and Parys, Eugeniusz and Gardeström, Per},
year = {2002},
note = {\_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1034/j.1399-3054.2002.1160203.x},
pages = {148--154},
}
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Pb2+ caused an increase in ATP content and the ATP/ADP ratio in pea and maize leaves. Rapid fractionation of barley protoplasts incubated at low and high CO2 conditions, indicated that the increased ATP/ADP ratio in Pb2+-treated leaves resulted mainly from the production of mitochondrial ATP. The measurements of membrane potential of mitochondria with a TPP+-sensitive electrode further showed that mitochondria isolated from Pb2+-treated leaves had at least as high membrane potential as mitochondria from control leaves. The activity of NAD-malate dehydrogenase in the protoplasts from barley leaves treated with Pb2+ was 3-fold higher than in protoplasts from control leaves. The activities of photorespiratory enzymes NADH-hydroxypyruvate reductase and glycolate oxidase as well as of NAD-malic enzyme were not affected. 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