@article{
 title = {Capital Breeding in a Diapausing Copepod: A Transcriptomics Analysis},
 type = {article},
 year = {2020},
 keywords = {Energy resources,Gene Expression,Neocalanus flemingeri,Oogenesis,RNA-Seq},
 pages = {56},
 volume = {7},
 websites = {https://www.frontiersin.org/article/10.3389/fmars.2020.00056/full},
 month = {3},
 publisher = {Frontiers},
 day = {6},
 id = {6bfc10a6-4435-3ef4-9aae-951c94ee2a93},
 created = {2020-04-29T21:57:54.675Z},
 accessed = {2020-03-06},
 file_attached = {false},
 profile_id = {5db6d3e7-562f-3ec2-a249-16ecf1e747e4},
 group_id = {bbf8f605-d7c8-3b07-85eb-dbce5212cc00},
 last_modified = {2020-04-29T21:57:54.732Z},
 read = {false},
 starred = {false},
 authored = {false},
 confirmed = {false},
 hidden = {false},
 private_publication = {false},
 abstract = {Capital breeders must balance the energetic requirements of cellular function and the cost of reproduction in organisms. The subarctic copepod Neocalanus flemingeri, a small planktonic crustacean, depends on a short annual phytoplankton bloom to acquire the energy needed to support a non-feeding female adult that enters a period of diapause prior to spawning five to seven months later. After emergence from diapause, the reproductive program takes two-months starting with germline development. The relationships between energy utilization, cellular maintenance and oogenesis were investigated through gene expression profiling. The sequential up- and down-regulation of genes involved in cellular maintenance and metabolic pathways coincided with four developmental groups: diapause, emergence from diapause and early oogenesis, mid to late oogenesis, and finally spawning and end-of-life. Up-regulation of genes involved in cellular homeostasis, glycolysis and lipid catabolism as well as germline development characterized the transition from diapause to post-diapause. The down-regulation of genes involved in cellular homeostasis coincided with the up-regulation of genes related to mid-late oogenesis and protein degradation. As females started to spawn, genes involved in protein ubiquitination and programmed cell death became up-regulated. Energy allocation and utilization is highly dynamic in N. flemingeri during the non-feeding period and it is linked to the provisioning and maturation of eggs through lipid catabolism and autophagy. The data suggest the presence of a mechanism that regulates fecundity to match the availability of stored resources. Such a mechanism would minimize reproductive failure when resources and contribute to the resilience of the species.},
 bibtype = {article},
 author = {Roncalli, Vittoria and Cieslak, Matthew C. and Hopcroft, Russell R. and Lenz, Petra H.},
 doi = {10.3389/fmars.2020.00056},
 journal = {Frontiers in Marine Science}
}

{"_id":"vZtomChHDkmPPKE4x","bibbaseid":"roncalli-cieslak-hopcroft-lenz-capitalbreedinginadiapausingcopepodatranscriptomicsanalysis-2020","authorIDs":[],"author_short":["Roncalli, V.","Cieslak, M., C.","Hopcroft, R., R.","Lenz, P., H."],"bibdata":{"title":"Capital Breeding in a Diapausing Copepod: A Transcriptomics Analysis","type":"article","year":"2020","keywords":"Energy resources,Gene Expression,Neocalanus flemingeri,Oogenesis,RNA-Seq","pages":"56","volume":"7","websites":"https://www.frontiersin.org/article/10.3389/fmars.2020.00056/full","month":"3","publisher":"Frontiers","day":"6","id":"6bfc10a6-4435-3ef4-9aae-951c94ee2a93","created":"2020-04-29T21:57:54.675Z","accessed":"2020-03-06","file_attached":false,"profile_id":"5db6d3e7-562f-3ec2-a249-16ecf1e747e4","group_id":"bbf8f605-d7c8-3b07-85eb-dbce5212cc00","last_modified":"2020-04-29T21:57:54.732Z","read":false,"starred":false,"authored":false,"confirmed":false,"hidden":false,"private_publication":false,"abstract":"Capital breeders must balance the energetic requirements of cellular function and the cost of reproduction in organisms. The subarctic copepod Neocalanus flemingeri, a small planktonic crustacean, depends on a short annual phytoplankton bloom to acquire the energy needed to support a non-feeding female adult that enters a period of diapause prior to spawning five to seven months later. After emergence from diapause, the reproductive program takes two-months starting with germline development. The relationships between energy utilization, cellular maintenance and oogenesis were investigated through gene expression profiling. The sequential up- and down-regulation of genes involved in cellular maintenance and metabolic pathways coincided with four developmental groups: diapause, emergence from diapause and early oogenesis, mid to late oogenesis, and finally spawning and end-of-life. Up-regulation of genes involved in cellular homeostasis, glycolysis and lipid catabolism as well as germline development characterized the transition from diapause to post-diapause. The down-regulation of genes involved in cellular homeostasis coincided with the up-regulation of genes related to mid-late oogenesis and protein degradation. As females started to spawn, genes involved in protein ubiquitination and programmed cell death became up-regulated. Energy allocation and utilization is highly dynamic in N. flemingeri during the non-feeding period and it is linked to the provisioning and maturation of eggs through lipid catabolism and autophagy. The data suggest the presence of a mechanism that regulates fecundity to match the availability of stored resources. Such a mechanism would minimize reproductive failure when resources and contribute to the resilience of the species.","bibtype":"article","author":"Roncalli, Vittoria and Cieslak, Matthew C. and Hopcroft, Russell R. and Lenz, Petra H.","doi":"10.3389/fmars.2020.00056","journal":"Frontiers in Marine Science","bibtex":"@article{\n title = {Capital Breeding in a Diapausing Copepod: A Transcriptomics Analysis},\n type = {article},\n year = {2020},\n keywords = {Energy resources,Gene Expression,Neocalanus flemingeri,Oogenesis,RNA-Seq},\n pages = {56},\n volume = {7},\n websites = {https://www.frontiersin.org/article/10.3389/fmars.2020.00056/full},\n month = {3},\n publisher = {Frontiers},\n day = {6},\n id = {6bfc10a6-4435-3ef4-9aae-951c94ee2a93},\n created = {2020-04-29T21:57:54.675Z},\n accessed = {2020-03-06},\n file_attached = {false},\n profile_id = {5db6d3e7-562f-3ec2-a249-16ecf1e747e4},\n group_id = {bbf8f605-d7c8-3b07-85eb-dbce5212cc00},\n last_modified = {2020-04-29T21:57:54.732Z},\n read = {false},\n starred = {false},\n authored = {false},\n confirmed = {false},\n hidden = {false},\n private_publication = {false},\n abstract = {Capital breeders must balance the energetic requirements of cellular function and the cost of reproduction in organisms. The subarctic copepod Neocalanus flemingeri, a small planktonic crustacean, depends on a short annual phytoplankton bloom to acquire the energy needed to support a non-feeding female adult that enters a period of diapause prior to spawning five to seven months later. After emergence from diapause, the reproductive program takes two-months starting with germline development. The relationships between energy utilization, cellular maintenance and oogenesis were investigated through gene expression profiling. The sequential up- and down-regulation of genes involved in cellular maintenance and metabolic pathways coincided with four developmental groups: diapause, emergence from diapause and early oogenesis, mid to late oogenesis, and finally spawning and end-of-life. Up-regulation of genes involved in cellular homeostasis, glycolysis and lipid catabolism as well as germline development characterized the transition from diapause to post-diapause. The down-regulation of genes involved in cellular homeostasis coincided with the up-regulation of genes related to mid-late oogenesis and protein degradation. As females started to spawn, genes involved in protein ubiquitination and programmed cell death became up-regulated. Energy allocation and utilization is highly dynamic in N. flemingeri during the non-feeding period and it is linked to the provisioning and maturation of eggs through lipid catabolism and autophagy. The data suggest the presence of a mechanism that regulates fecundity to match the availability of stored resources. Such a mechanism would minimize reproductive failure when resources and contribute to the resilience of the species.},\n bibtype = {article},\n author = {Roncalli, Vittoria and Cieslak, Matthew C. and Hopcroft, Russell R. and Lenz, Petra H.},\n doi = {10.3389/fmars.2020.00056},\n journal = {Frontiers in Marine Science}\n}","author_short":["Roncalli, V.","Cieslak, M., C.","Hopcroft, R., R.","Lenz, P., H."],"urls":{"Website":"https://www.frontiersin.org/article/10.3389/fmars.2020.00056/full"},"biburl":"https://bibbase.org/service/mendeley/5db6d3e7-562f-3ec2-a249-16ecf1e747e4","bibbaseid":"roncalli-cieslak-hopcroft-lenz-capitalbreedinginadiapausingcopepodatranscriptomicsanalysis-2020","role":"author","keyword":["Energy resources","Gene Expression","Neocalanus flemingeri","Oogenesis","RNA-Seq"],"metadata":{"authorlinks":{}},"downloads":0},"bibtype":"article","creationDate":"2020-04-29T21:11:33.707Z","downloads":0,"keywords":["energy resources","gene expression","neocalanus flemingeri","oogenesis","rna-seq"],"search_terms":["capital","breeding","diapausing","copepod","transcriptomics","analysis","roncalli","cieslak","hopcroft","lenz"],"title":"Capital Breeding in a Diapausing Copepod: A Transcriptomics Analysis","year":2020,"biburl":"https://bibbase.org/service/mendeley/5db6d3e7-562f-3ec2-a249-16ecf1e747e4","dataSources":["peQMNPSdReHoCd34f","ya2CyA73rpZseyrZ8"]}