DNA polymerase-associated and autonomous 3′→5′ exonucleases in invertebrates, unicellulars, and bacteria

DNA polymerase-associated and autonomous 3′→5′ exonucleases in invertebrates, unicellulars, and bacteria. Ronzhina, N., Belyakova, N., Kravetskaya, T., & Krutyakov, V. Journal of Evolutionary Biochemistry and Physiology, 38(2):161-168, 2002. cited By 0

Paper doi abstract bibtex

Recently we have revealed a high content of autonomous 3′→ 5′ exonucleases (AE), i.e., those not bound covalently with DNA polymerases, in cells of vertebrates, from fish to human [1]. In the present work, using gel filtration method, cell-free extracts were studied from 15 objects located at different positions on the phylogenetic tree, such as archaebacteria, eubacteria, fungi, infusorians, coelenterates, annelids, and arthropods. It is shown that enzymatic activity of AE accounts for from 30 to 88% of the total 3′→5′ exonuclease activity of the extracts. A part of AE is revealed in zone of high-molecular DNA polymerases and can be separated by change of the chromatography conditions. It indicates a probable formation of complexes of AE with DNA polymerases. The high AE activity in cells of different organisms, from archae- and eubacteria to human, allows suggesting these enzymes to play a significant role in correction of polymerase errors in the processes of DNA replication and reparation, as well as in postreplicative correction of heteroduplexes in DNA.

@ARTICLE{Ronzhina2002161,
author={Ronzhina, N.L. and Belyakova, N.V. and Kravetskaya, T.P. and Krutyakov, V.M.},
title={DNA polymerase-associated and autonomous 3′→5′ exonucleases in invertebrates, unicellulars, and bacteria},
journal={Journal of Evolutionary Biochemistry and Physiology},
year={2002},
volume={38},
number={2},
pages={161-168},
doi={10.1023/A:1016502303352},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-9644274328&doi=10.1023%2fA%3a1016502303352&partnerID=40&md5=f8c1eb010fc4d101bb3e022d11d36d01},
affiliation={Konstantinov St. Petersburg Inst. N., Russian Academy of Science, Gatchina, Russia, Russian Federation},
abstract={Recently we have revealed a high content of autonomous 3′→ 5′ exonucleases (AE), i.e., those not bound covalently with DNA polymerases, in cells of vertebrates, from fish to human [1]. In the present work, using gel filtration method, cell-free extracts were studied from 15 objects located at different positions on the phylogenetic tree, such as archaebacteria, eubacteria, fungi, infusorians, coelenterates, annelids, and arthropods. It is shown that enzymatic activity of AE accounts for from 30 to 88% of the total 3′→5′ exonuclease activity of the extracts. A part of AE is revealed in zone of high-molecular DNA polymerases and can be separated by change of the chromatography conditions. It indicates a probable formation of complexes of AE with DNA polymerases. The high AE activity in cells of different organisms, from archae- and eubacteria to human, allows suggesting these enzymes to play a significant role in correction of polymerase errors in the processes of DNA replication and reparation, as well as in postreplicative correction of heteroduplexes in DNA.},
correspondence_address1={Konstantinov St. Petersburg Inst. N., Russian Academy of Science, Gatchina, RussiaRussian Federation},
issn={00220930},
coden={JEBPA},
language={English},
abbrev_source_title={J. Evol. Biochem. Physiol.},
document_type={Article},
source={Scopus},
}

Downloads: 0

{"_id":"vz4kAWWB3jPAoFYn8","bibbaseid":"ronzhina-belyakova-kravetskaya-krutyakov-dnapolymeraseassociatedandautonomous35exonucleasesininvertebratesunicellularsandbacteria-2002","author_short":["Ronzhina, N.","Belyakova, N.","Kravetskaya, T.","Krutyakov, V."],"bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Ronzhina"],"firstnames":["N.L."],"suffixes":[]},{"propositions":[],"lastnames":["Belyakova"],"firstnames":["N.V."],"suffixes":[]},{"propositions":[],"lastnames":["Kravetskaya"],"firstnames":["T.P."],"suffixes":[]},{"propositions":[],"lastnames":["Krutyakov"],"firstnames":["V.M."],"suffixes":[]}],"title":"DNA polymerase-associated and autonomous 3′→5′ exonucleases in invertebrates, unicellulars, and bacteria","journal":"Journal of Evolutionary Biochemistry and Physiology","year":"2002","volume":"38","number":"2","pages":"161-168","doi":"10.1023/A:1016502303352","note":"cited By 0","url":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-9644274328&doi=10.1023%2fA%3a1016502303352&partnerID=40&md5=f8c1eb010fc4d101bb3e022d11d36d01","affiliation":"Konstantinov St. Petersburg Inst. N., Russian Academy of Science, Gatchina, Russia, Russian Federation","abstract":"Recently we have revealed a high content of autonomous 3′→ 5′ exonucleases (AE), i.e., those not bound covalently with DNA polymerases, in cells of vertebrates, from fish to human [1]. In the present work, using gel filtration method, cell-free extracts were studied from 15 objects located at different positions on the phylogenetic tree, such as archaebacteria, eubacteria, fungi, infusorians, coelenterates, annelids, and arthropods. It is shown that enzymatic activity of AE accounts for from 30 to 88% of the total 3′→5′ exonuclease activity of the extracts. A part of AE is revealed in zone of high-molecular DNA polymerases and can be separated by change of the chromatography conditions. It indicates a probable formation of complexes of AE with DNA polymerases. The high AE activity in cells of different organisms, from archae- and eubacteria to human, allows suggesting these enzymes to play a significant role in correction of polymerase errors in the processes of DNA replication and reparation, as well as in postreplicative correction of heteroduplexes in DNA.","correspondence_address1":"Konstantinov St. Petersburg Inst. N., Russian Academy of Science, Gatchina, RussiaRussian Federation","issn":"00220930","coden":"JEBPA","language":"English","abbrev_source_title":"J. Evol. Biochem. Physiol.","document_type":"Article","source":"Scopus","bibtex":"@ARTICLE{Ronzhina2002161,\r\nauthor={Ronzhina, N.L. and Belyakova, N.V. and Kravetskaya, T.P. and Krutyakov, V.M.},\r\ntitle={DNA polymerase-associated and autonomous 3′→5′ exonucleases in invertebrates, unicellulars, and bacteria},\r\njournal={Journal of Evolutionary Biochemistry and Physiology},\r\nyear={2002},\r\nvolume={38},\r\nnumber={2},\r\npages={161-168},\r\ndoi={10.1023/A:1016502303352},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-9644274328&doi=10.1023%2fA%3a1016502303352&partnerID=40&md5=f8c1eb010fc4d101bb3e022d11d36d01},\r\naffiliation={Konstantinov St. Petersburg Inst. N., Russian Academy of Science, Gatchina, Russia, Russian Federation},\r\nabstract={Recently we have revealed a high content of autonomous 3′→ 5′ exonucleases (AE), i.e., those not bound covalently with DNA polymerases, in cells of vertebrates, from fish to human [1]. In the present work, using gel filtration method, cell-free extracts were studied from 15 objects located at different positions on the phylogenetic tree, such as archaebacteria, eubacteria, fungi, infusorians, coelenterates, annelids, and arthropods. It is shown that enzymatic activity of AE accounts for from 30 to 88% of the total 3′→5′ exonuclease activity of the extracts. A part of AE is revealed in zone of high-molecular DNA polymerases and can be separated by change of the chromatography conditions. It indicates a probable formation of complexes of AE with DNA polymerases. The high AE activity in cells of different organisms, from archae- and eubacteria to human, allows suggesting these enzymes to play a significant role in correction of polymerase errors in the processes of DNA replication and reparation, as well as in postreplicative correction of heteroduplexes in DNA.},\r\ncorrespondence_address1={Konstantinov St. Petersburg Inst. N., Russian Academy of Science, Gatchina, RussiaRussian Federation},\r\nissn={00220930},\r\ncoden={JEBPA},\r\nlanguage={English},\r\nabbrev_source_title={J. Evol. Biochem. Physiol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n","author_short":["Ronzhina, N.","Belyakova, N.","Kravetskaya, T.","Krutyakov, V."],"key":"Ronzhina2002161","id":"Ronzhina2002161","bibbaseid":"ronzhina-belyakova-kravetskaya-krutyakov-dnapolymeraseassociatedandautonomous35exonucleasesininvertebratesunicellularsandbacteria-2002","role":"author","urls":{"Paper":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-9644274328&doi=10.1023%2fA%3a1016502303352&partnerID=40&md5=f8c1eb010fc4d101bb3e022d11d36d01"},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bio.pnpi.nrcki.ru/wp-content/uploads/2019/12/lpro_2019_10.txt","dataSources":["eoAALb5LHAEShrPHa"],"keywords":[],"search_terms":["dna","polymerase","associated","autonomous","exonucleases","invertebrates","unicellulars","bacteria","ronzhina","belyakova","kravetskaya","krutyakov"],"title":"DNA polymerase-associated and autonomous 3′→5′ exonucleases in invertebrates, unicellulars, and bacteria","year":2002}