Mechanical injury of bovine cartilage explants induces depth-dependent, transient changes in MAP kinase activity associated with apoptosis. Rosenzweig, D. H., Djap, M. J., Ou, S. J., & Quinn, T. M. Osteoarthritis and Cartilage, 20(12):1591–1602, 2012. doi abstract bibtex Objective: To characterize mitogen activated protein (MAP) kinase activity and chondrocyte apoptosis in an in vitro model of cartilage mechanical injury as a function of tissue depth and time post-injury. Design: Mechanically injured osteochondral explants were assessed for cell viability, MAP kinase and caspase-3 activity over 15 days using immunofluorescence microscopy and Western blot. Zonal distributions of cell viability and apoptosis were quantified in the presence of specific mitogen activated protein kinase inhibitors. Results: Viability rapidly decreased post-injury, most significantly in the superficial zone, with some involvement of the middle and deep zones, which correlated with increased caspase-3 activity. Transient and significant increases in extracellular-regulated protein kinase (ERK) activity were observed in middle and deep zones at 1 and 6 days post-injury, while c-Jun-amino terminal protein kinase activity increased in the deep zone at 1 and 6 days compared to uninjured controls. Changes in p38 activity were particularly pronounced, with significant increases in all three zones 30 min post-injury, but only in the middle and deep zones after 1 and 6 days. Inhibition of ERK and p38 increased chondrocyte viability which correlated with decreased apoptosis. Conclusions: Spatiotemporal patterns of MAP kinase signalling in cartilage after mechanical injury strongly correlate with changes in cell viability and chondrocyte apoptosis. Importantly, these signals may be pro-survival or pro-apoptotic depending on zonal location and time post-injury. These data yield mechanistic insights which may improve the diagnosis and treatment of cartilage injuries. (C) 2012 Osteoarthritis Research Society International.
@article{Rosenzweig20121591,
abstract = {Objective: To characterize mitogen activated protein (MAP) kinase activity and chondrocyte apoptosis in an in vitro model of cartilage mechanical injury as a function of tissue depth and time post-injury. Design: Mechanically injured osteochondral explants were assessed for cell viability, MAP kinase and caspase-3 activity over 15 days using immunofluorescence microscopy and Western blot. Zonal distributions of cell viability and apoptosis were quantified in the presence of specific mitogen activated protein kinase inhibitors. Results: Viability rapidly decreased post-injury, most significantly in the superficial zone, with some involvement of the middle and deep zones, which correlated with increased caspase-3 activity. Transient and significant increases in extracellular-regulated protein kinase (ERK) activity were observed in middle and deep zones at 1 and 6 days post-injury, while c-Jun-amino terminal protein kinase activity increased in the deep zone at 1 and 6 days compared to uninjured controls. Changes in p38 activity were particularly pronounced, with significant increases in all three zones 30 min post-injury, but only in the middle and deep zones after 1 and 6 days. Inhibition of ERK and p38 increased chondrocyte viability which correlated with decreased apoptosis. Conclusions: Spatiotemporal patterns of MAP kinase signalling in cartilage after mechanical injury strongly correlate with changes in cell viability and chondrocyte apoptosis. Importantly, these signals may be pro-survival or pro-apoptotic depending on zonal location and time post-injury. These data yield mechanistic insights which may improve the diagnosis and treatment of cartilage injuries. (C) 2012 Osteoarthritis Research Society International.},
annote = {cited By 19},
author = {Rosenzweig, D. H. and Djap, M. J. and Ou, S. J. and Quinn, T. M.},
doi = {10.1016/j.joca.2012.08.012},
issn = {10634584},
journal = {Osteoarthritis and Cartilage},
keywords = {Chondrocyte,Compression,Gene expression,Injury,MAP kinase,Signalling},
number = {12},
pages = {1591--1602},
pmid = {22935788},
title = {{Mechanical injury of bovine cartilage explants induces depth-dependent, transient changes in MAP kinase activity associated with apoptosis}},
volume = {20},
year = {2012}
}
Downloads: 0
{"_id":"3AKDSWczDpWtaZZhk","bibbaseid":"rosenzweig-djap-ou-quinn-mechanicalinjuryofbovinecartilageexplantsinducesdepthdependenttransientchangesinmapkinaseactivityassociatedwithapoptosis-2012","authorIDs":["7AZ96jedDgXtbS2gZ","Aatxv2tJWMncBRk2u","GCSP5Bv7HRSfvTgtF","HjeQr6ipAQDCTb34Y","JXLnXyXovDQzK8jhE","QZJeRuRbo2f3Tktid","QftFYMfuBhvSrqkqw","RE87xEGJag3ckDR39","YmjqcmFRu2TrTQikj","soZgPGDyH8ywPF7Wh","yLsyWNQShJByQdMwZ","yYsb2kRc5GjCKdqk3"],"author_short":["Rosenzweig, D. H.","Djap, M. J.","Ou, S. J.","Quinn, T. M."],"bibdata":{"bibtype":"article","type":"article","abstract":"Objective: To characterize mitogen activated protein (MAP) kinase activity and chondrocyte apoptosis in an in vitro model of cartilage mechanical injury as a function of tissue depth and time post-injury. Design: Mechanically injured osteochondral explants were assessed for cell viability, MAP kinase and caspase-3 activity over 15 days using immunofluorescence microscopy and Western blot. Zonal distributions of cell viability and apoptosis were quantified in the presence of specific mitogen activated protein kinase inhibitors. Results: Viability rapidly decreased post-injury, most significantly in the superficial zone, with some involvement of the middle and deep zones, which correlated with increased caspase-3 activity. Transient and significant increases in extracellular-regulated protein kinase (ERK) activity were observed in middle and deep zones at 1 and 6 days post-injury, while c-Jun-amino terminal protein kinase activity increased in the deep zone at 1 and 6 days compared to uninjured controls. Changes in p38 activity were particularly pronounced, with significant increases in all three zones 30 min post-injury, but only in the middle and deep zones after 1 and 6 days. Inhibition of ERK and p38 increased chondrocyte viability which correlated with decreased apoptosis. Conclusions: Spatiotemporal patterns of MAP kinase signalling in cartilage after mechanical injury strongly correlate with changes in cell viability and chondrocyte apoptosis. Importantly, these signals may be pro-survival or pro-apoptotic depending on zonal location and time post-injury. These data yield mechanistic insights which may improve the diagnosis and treatment of cartilage injuries. (C) 2012 Osteoarthritis Research Society International.","annote":"cited By 19","author":[{"propositions":[],"lastnames":["Rosenzweig"],"firstnames":["D.","H."],"suffixes":[]},{"propositions":[],"lastnames":["Djap"],"firstnames":["M.","J."],"suffixes":[]},{"propositions":[],"lastnames":["Ou"],"firstnames":["S.","J."],"suffixes":[]},{"propositions":[],"lastnames":["Quinn"],"firstnames":["T.","M."],"suffixes":[]}],"doi":"10.1016/j.joca.2012.08.012","issn":"10634584","journal":"Osteoarthritis and Cartilage","keywords":"Chondrocyte,Compression,Gene expression,Injury,MAP kinase,Signalling","number":"12","pages":"1591–1602","pmid":"22935788","title":"Mechanical injury of bovine cartilage explants induces depth-dependent, transient changes in MAP kinase activity associated with apoptosis","volume":"20","year":"2012","bibtex":"@article{Rosenzweig20121591,\n abstract = {Objective: To characterize mitogen activated protein (MAP) kinase activity and chondrocyte apoptosis in an in vitro model of cartilage mechanical injury as a function of tissue depth and time post-injury. Design: Mechanically injured osteochondral explants were assessed for cell viability, MAP kinase and caspase-3 activity over 15 days using immunofluorescence microscopy and Western blot. Zonal distributions of cell viability and apoptosis were quantified in the presence of specific mitogen activated protein kinase inhibitors. Results: Viability rapidly decreased post-injury, most significantly in the superficial zone, with some involvement of the middle and deep zones, which correlated with increased caspase-3 activity. Transient and significant increases in extracellular-regulated protein kinase (ERK) activity were observed in middle and deep zones at 1 and 6 days post-injury, while c-Jun-amino terminal protein kinase activity increased in the deep zone at 1 and 6 days compared to uninjured controls. Changes in p38 activity were particularly pronounced, with significant increases in all three zones 30 min post-injury, but only in the middle and deep zones after 1 and 6 days. Inhibition of ERK and p38 increased chondrocyte viability which correlated with decreased apoptosis. Conclusions: Spatiotemporal patterns of MAP kinase signalling in cartilage after mechanical injury strongly correlate with changes in cell viability and chondrocyte apoptosis. Importantly, these signals may be pro-survival or pro-apoptotic depending on zonal location and time post-injury. These data yield mechanistic insights which may improve the diagnosis and treatment of cartilage injuries. (C) 2012 Osteoarthritis Research Society International.},\n annote = {cited By 19},\n author = {Rosenzweig, D. H. and Djap, M. J. and Ou, S. J. and Quinn, T. M.},\n doi = {10.1016/j.joca.2012.08.012},\n issn = {10634584},\n journal = {Osteoarthritis and Cartilage},\n keywords = {Chondrocyte,Compression,Gene expression,Injury,MAP kinase,Signalling},\n number = {12},\n pages = {1591--1602},\n pmid = {22935788},\n title = {{Mechanical injury of bovine cartilage explants induces depth-dependent, transient changes in MAP kinase activity associated with apoptosis}},\n volume = {20},\n year = {2012}\n }\n ","author_short":["Rosenzweig, D. H.","Djap, M. J.","Ou, S. J.","Quinn, T. M."],"key":"Rosenzweig20121591","id":"Rosenzweig20121591","bibbaseid":"rosenzweig-djap-ou-quinn-mechanicalinjuryofbovinecartilageexplantsinducesdepthdependenttransientchangesinmapkinaseactivityassociatedwithapoptosis-2012","role":"author","urls":{},"keyword":["Chondrocyte","Compression","Gene expression","Injury","MAP kinase","Signalling"],"metadata":{"authorlinks":{"rosenzweig, d":"https://rosenzweiglab.com/publication/"}},"downloads":0},"bibtype":"article","creationDate":"2020-05-22T18:05:19.781Z","downloads":0,"keywords":["chondrocyte","compression","gene expression","injury","map kinase","signalling"],"search_terms":["mechanical","injury","bovine","cartilage","explants","induces","depth","dependent","transient","changes","map","kinase","activity","associated","apoptosis","rosenzweig","djap","ou","quinn"],"title":"Mechanical injury of bovine cartilage explants induces depth-dependent, transient changes in MAP kinase activity associated with apoptosis","year":2012,"biburl":"https://raw.githubusercontent.com/rosenzweiglab/rosenzweiglab.github.io/master/docs/references.bib","dataSources":["2AaMQS2PLCP3KCSxQ"]}