@article{RN201,
   author = {Rossmeisl, J. H., Jr. and Garcia, P. A. and Roberston, J. L. and Ellis, T. L. and Davalos, R. V.},
   title = {Pathology of non-thermal irreversible electroporation (N-TIRE)-induced ablation of the canine brain},
   journal = {J Vet Sci},
   volume = {14},
   number = {4},
   pages = {433-40},
   note = {1976-555x
Rossmeisl, John H Jr
Garcia, Paulo A
Roberston, John L
Ellis, Thomas L
Davalos, Rafael V
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Korea (South)
2013/07/04
J Vet Sci. 2013;14(4):433-40. doi: 10.4142/jvs.2013.14.4.433. Epub 2013 Jun 28.},
   abstract = {This study describes the neuropathologic features of normal canine brain ablated with non-thermal irreversible electroporation (N-TIRE). The parietal cerebral cortices of four dogs were treated with N-TIRE using a dose-escalation protocol with an additional dog receiving sham treatment. Animals were allowed to recover following N-TIRE ablation and the effects of treatment were monitored with clinical and magnetic resonance imaging examinations. Brains were subjected to histopathologic and ultrastructural assessment along with Bcl-2, caspase-3, and caspase-9 immunohistochemical staining following sacrifice 72 h post-treatment. Adverse clinical effects of N-TIRE were only observed in the dog treated at the upper energy tier. MRI and neuropathologic examinations indicated that N-TIRE ablation resulted in focal regions of severe cytoarchitectural and blood-brain-barrier disruption. Lesion size correlated to the intensity of the applied electrical field. N-TIRE-induced lesions were characterized by parenchymal necrosis and hemorrhage; however, large blood vessels were preserved. A transition zone containing parenchymal edema, perivascular inflammatory cuffs, and reactive gliosis was interspersed between the necrotic focus and normal neuropil. Apoptotic labeling indices were not different between the N-TIRE-treated and control brains. This study identified N-TIRE pulse parameters that can be used to safely create circumscribed foci of brain necrosis while selectively preserving major vascular structures.},
   keywords = {Animals
Brain/metabolism/*pathology/surgery/ultrastructure
Caspase 3/metabolism
Caspase 9/metabolism
Dogs
Electroporation/veterinary
Magnetic Resonance Imaging/methods
Microscopy, Electron, Transmission
Necrosis/metabolism/pathology
Neurosurgical Procedures/*adverse effects
central nervous system
dog
irreversible electroporation
neuropathology},
   ISSN = {1229-845X (Print)
1229-845x},
   DOI = {10.4142/jvs.2013.14.4.433},
   year = {2013},
   type = {Journal Article}
}

{"_id":"SuC8H4X6EKek57u5z","bibbaseid":"rossmeisl-garcia-roberston-ellis-davalos-pathologyofnonthermalirreversibleelectroporationntireinducedablationofthecaninebrain-2013","author_short":["Rossmeisl, J. H.","Garcia, P. A.","Roberston, J. L.","Ellis, T. L.","Davalos, R. V."],"bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["Rossmeisl"],"firstnames":["J.","H."],"suffixes":["Jr."]},{"propositions":[],"lastnames":["Garcia"],"firstnames":["P.","A."],"suffixes":[]},{"propositions":[],"lastnames":["Roberston"],"firstnames":["J.","L."],"suffixes":[]},{"propositions":[],"lastnames":["Ellis"],"firstnames":["T.","L."],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["R.","V."],"suffixes":[]}],"title":"Pathology of non-thermal irreversible electroporation (N-TIRE)-induced ablation of the canine brain","journal":"J Vet Sci","volume":"14","number":"4","pages":"433-40","note":"1976-555x Rossmeisl, John H Jr Garcia, Paulo A Roberston, John L Ellis, Thomas L Davalos, Rafael V Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Korea (South) 2013/07/04 J Vet Sci. 2013;14(4):433-40. doi: 10.4142/jvs.2013.14.4.433. Epub 2013 Jun 28.","abstract":"This study describes the neuropathologic features of normal canine brain ablated with non-thermal irreversible electroporation (N-TIRE). The parietal cerebral cortices of four dogs were treated with N-TIRE using a dose-escalation protocol with an additional dog receiving sham treatment. Animals were allowed to recover following N-TIRE ablation and the effects of treatment were monitored with clinical and magnetic resonance imaging examinations. Brains were subjected to histopathologic and ultrastructural assessment along with Bcl-2, caspase-3, and caspase-9 immunohistochemical staining following sacrifice 72 h post-treatment. Adverse clinical effects of N-TIRE were only observed in the dog treated at the upper energy tier. MRI and neuropathologic examinations indicated that N-TIRE ablation resulted in focal regions of severe cytoarchitectural and blood-brain-barrier disruption. Lesion size correlated to the intensity of the applied electrical field. N-TIRE-induced lesions were characterized by parenchymal necrosis and hemorrhage; however, large blood vessels were preserved. A transition zone containing parenchymal edema, perivascular inflammatory cuffs, and reactive gliosis was interspersed between the necrotic focus and normal neuropil. Apoptotic labeling indices were not different between the N-TIRE-treated and control brains. This study identified N-TIRE pulse parameters that can be used to safely create circumscribed foci of brain necrosis while selectively preserving major vascular structures.","keywords":"Animals Brain/metabolism/*pathology/surgery/ultrastructure Caspase 3/metabolism Caspase 9/metabolism Dogs Electroporation/veterinary Magnetic Resonance Imaging/methods Microscopy, Electron, Transmission Necrosis/metabolism/pathology Neurosurgical Procedures/*adverse effects central nervous system dog irreversible electroporation neuropathology","issn":"1229-845X (Print) 1229-845x","doi":"10.4142/jvs.2013.14.4.433","year":"2013","bibtex":"@article{RN201,\n author = {Rossmeisl, J. H., Jr. and Garcia, P. A. and Roberston, J. L. and Ellis, T. L. and Davalos, R. V.},\n title = {Pathology of non-thermal irreversible electroporation (N-TIRE)-induced ablation of the canine brain},\n journal = {J Vet Sci},\n volume = {14},\n number = {4},\n pages = {433-40},\n note = {1976-555x\nRossmeisl, John H Jr\nGarcia, Paulo A\nRoberston, John L\nEllis, Thomas L\nDavalos, Rafael V\nJournal Article\nResearch Support, Non-U.S. Gov't\nResearch Support, U.S. Gov't, Non-P.H.S.\nKorea (South)\n2013/07/04\nJ Vet Sci. 2013;14(4):433-40. doi: 10.4142/jvs.2013.14.4.433. Epub 2013 Jun 28.},\n abstract = {This study describes the neuropathologic features of normal canine brain ablated with non-thermal irreversible electroporation (N-TIRE). The parietal cerebral cortices of four dogs were treated with N-TIRE using a dose-escalation protocol with an additional dog receiving sham treatment. Animals were allowed to recover following N-TIRE ablation and the effects of treatment were monitored with clinical and magnetic resonance imaging examinations. Brains were subjected to histopathologic and ultrastructural assessment along with Bcl-2, caspase-3, and caspase-9 immunohistochemical staining following sacrifice 72 h post-treatment. Adverse clinical effects of N-TIRE were only observed in the dog treated at the upper energy tier. MRI and neuropathologic examinations indicated that N-TIRE ablation resulted in focal regions of severe cytoarchitectural and blood-brain-barrier disruption. Lesion size correlated to the intensity of the applied electrical field. N-TIRE-induced lesions were characterized by parenchymal necrosis and hemorrhage; however, large blood vessels were preserved. A transition zone containing parenchymal edema, perivascular inflammatory cuffs, and reactive gliosis was interspersed between the necrotic focus and normal neuropil. Apoptotic labeling indices were not different between the N-TIRE-treated and control brains. This study identified N-TIRE pulse parameters that can be used to safely create circumscribed foci of brain necrosis while selectively preserving major vascular structures.},\n keywords = {Animals\nBrain/metabolism/*pathology/surgery/ultrastructure\nCaspase 3/metabolism\nCaspase 9/metabolism\nDogs\nElectroporation/veterinary\nMagnetic Resonance Imaging/methods\nMicroscopy, Electron, Transmission\nNecrosis/metabolism/pathology\nNeurosurgical Procedures/*adverse effects\ncentral nervous system\ndog\nirreversible electroporation\nneuropathology},\n ISSN = {1229-845X (Print)\n1229-845x},\n DOI = {10.4142/jvs.2013.14.4.433},\n year = {2013},\n type = {Journal Article}\n}\n\n","author_short":["Rossmeisl, J. H.","Garcia, P. A.","Roberston, J. L.","Ellis, T. L.","Davalos, R. V."],"key":"RN201","id":"RN201","bibbaseid":"rossmeisl-garcia-roberston-ellis-davalos-pathologyofnonthermalirreversibleelectroporationntireinducedablationofthecaninebrain-2013","role":"author","urls":{},"keyword":["Animals Brain/metabolism/*pathology/surgery/ultrastructure Caspase 3/metabolism Caspase 9/metabolism Dogs Electroporation/veterinary Magnetic Resonance Imaging/methods Microscopy","Electron","Transmission Necrosis/metabolism/pathology Neurosurgical Procedures/*adverse effects central nervous system dog irreversible electroporation neuropathology"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/network/files/bdNBTZRXTsoHCgpbh","dataSources":["D4zENc4BfFNBwSYYJ","ZPLjameRikygaiM9B","3XfNmZkLe6o8CvECW","fJQsxtBoqymHQG6tL","LzxgEApraxMPkLTMn","Z2THpXfLYEJf3CB8p"],"keywords":["animals brain/metabolism/*pathology/surgery/ultrastructure caspase 3/metabolism caspase 9/metabolism dogs electroporation/veterinary magnetic resonance imaging/methods microscopy","electron","transmission necrosis/metabolism/pathology neurosurgical procedures/*adverse effects central nervous system dog irreversible electroporation neuropathology"],"search_terms":["pathology","non","thermal","irreversible","electroporation","tire","induced","ablation","canine","brain","rossmeisl","garcia","roberston","ellis","davalos"],"title":"Pathology of non-thermal irreversible electroporation (N-TIRE)-induced ablation of the canine brain","year":2013}