@article{roullier_time_2016,
	title = {Time Dependency of Chemodiversity and Biosynthetic Pathways: An {LC}-{MS} Metabolomic Study of Marine-Sourced Penicillium},
	volume = {14},
	issn = {1660-3397},
	url = {http://www.mdpi.com/1660-3397/14/5/103},
	doi = {10.3390/md14050103},
	shorttitle = {Time Dependency of Chemodiversity and Biosynthetic Pathways},
	abstract = {This work aimed at studying metabolome variations of marine fungal strains along their growth to highlight the importance of the parameter “time” for new natural products discovery. An untargeted time-scale metabolomic study has been performed on two different marine-derived Penicillium strains. They were cultivated for 18 days and their crude extracts were analyzed by {HPLC}-{DAD}-{HRMS} (High Performance Liquid Chromatography-Diode Array Detector-High Resolution Mass Spectrometry) each day. With the example of griseofulvin biosynthesis, a pathway shared by both strains, this work provides a new approach to study biosynthetic pathway regulations, which could be applied to other metabolites and more particularly new ones. Moreover, the results of this study emphasize the interest of such an approach for the discovery of new chemical entities. In particular, at every harvesting time, previously undetected features were observed in the {LC}-{MS} (Liquid Chromatography-Mass Spectrometry) data. Therefore, harvesting times for metabolite extraction should be performed at different time points to access the hidden metabolome.},
	pages = {103},
	number = {5},
	journaltitle = {Marine Drugs},
	author = {Roullier, Catherine and Bertrand, Samuel and Blanchet, Elodie and Peigné, Mathilde and Robiou du Pont, Thibaut and Guitton, Yann and Pouchus, Yves and Grovel, Olivier},
	urldate = {2019-03-29},
	date = {2016-05-21},
	langid = {english},
	file = {Roullier et al. - 2016 - Time Dependency of Chemodiversity and Biosynthetic.pdf:C\:\\Users\\ygu\\Documents\\PCPOR066_YGU\\YGU\\Zotero\\storage\\A5XG3FD9\\Roullier et al. - 2016 - Time Dependency of Chemodiversity and Biosynthetic.pdf:application/pdf}
}

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An untargeted time-scale metabolomic study has been performed on two different marine-derived Penicillium strains. They were cultivated for 18 days and their crude extracts were analyzed by HPLC-DAD-HRMS (High Performance Liquid Chromatography-Diode Array Detector-High Resolution Mass Spectrometry) each day. With the example of griseofulvin biosynthesis, a pathway shared by both strains, this work provides a new approach to study biosynthetic pathway regulations, which could be applied to other metabolites and more particularly new ones. Moreover, the results of this study emphasize the interest of such an approach for the discovery of new chemical entities. In particular, at every harvesting time, previously undetected features were observed in the LC-MS (Liquid Chromatography-Mass Spectrometry) data. 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