@article{routier_investigating_2024,
	title = {Investigating the {Effect} of {Syringe} {Infiltration} on {Nicotiana} tabacum ({Tobacco})},
	volume = {5},
	issn = {2692-1952},
	url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11752493/},
	doi = {10.1021/acsagscitech.4c00170},
	abstract = {, Plant infiltration techniques, particularly agroinfiltration,
have
transformed plant science and biotechnology by enabling transient
gene expression for genetic engineering of plants or genomic studies.
Recently, the use of infiltration has expanded to introduce nanomaterials
and polymers in plants to enable nonnative functionalities. Despite
its wide use, the impact of the infiltration process per se on plant physiology needs to be better understood. This study investigates
the effect of syringe infiltration, a commonly employed technique
in plants, using a typical infiltration buffer solution. Noninvasive
and real-time monitoring methods, including high-resolution thermal
imaging and a porometer/fluorometer, were used to study the physiological
responses and stress levels of the infiltrated plants. Our results
revealed localized cell damage at the infiltration site due to syringe
compression, but the overall cell viability and tissue integrity were
largely unaffected. Thermography showed a temporary temperature increase
of the leaves and stomatal conductance alterations postinfiltration,
with leaf recovery in 3–6 days. Additionally, fluorescence
measurements indicated a 6\% decrease in maximum quantum efficiency
(Fv/Fm) and
a 34\% decrease in photosystem II (ΦPSII) quantum yield, persisting
for 5 days after infiltration, suggesting sustained photosystem efficiency
changes. Our work highlights the need to consider the effect of infiltration
when performing biological studies and aims to facilitate the optimization
of protocols commonly used in plant science and biotechnology.},
	number = {1},
	urldate = {2025-01-31},
	journal = {ACS Agricultural Science \& Technology},
	author = {Routier, Cyril and Hermida-Carrera, Carmen and Stavrinidou, Eleni},
	month = dec,
	year = {2024},
	pmid = {39850806},
	pmcid = {PMC11752493},
	pages = {28--35},
}

{"_id":"Q79oSSBqib8BnwWPy","bibbaseid":"routier-hermidacarrera-stavrinidou-investigatingtheeffectofsyringeinfiltrationonnicotianatabacumtobacco-2024","author_short":["Routier, C.","Hermida-Carrera, C.","Stavrinidou, E."],"bibdata":{"bibtype":"article","type":"article","title":"Investigating the Effect of Syringe Infiltration on Nicotiana tabacum (Tobacco)","volume":"5","issn":"2692-1952","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11752493/","doi":"10.1021/acsagscitech.4c00170","abstract":", Plant infiltration techniques, particularly agroinfiltration, have transformed plant science and biotechnology by enabling transient gene expression for genetic engineering of plants or genomic studies. Recently, the use of infiltration has expanded to introduce nanomaterials and polymers in plants to enable nonnative functionalities. Despite its wide use, the impact of the infiltration process per se on plant physiology needs to be better understood. This study investigates the effect of syringe infiltration, a commonly employed technique in plants, using a typical infiltration buffer solution. Noninvasive and real-time monitoring methods, including high-resolution thermal imaging and a porometer/fluorometer, were used to study the physiological responses and stress levels of the infiltrated plants. Our results revealed localized cell damage at the infiltration site due to syringe compression, but the overall cell viability and tissue integrity were largely unaffected. Thermography showed a temporary temperature increase of the leaves and stomatal conductance alterations postinfiltration, with leaf recovery in 3–6 days. Additionally, fluorescence measurements indicated a 6% decrease in maximum quantum efficiency (Fv/Fm) and a 34% decrease in photosystem II (ΦPSII) quantum yield, persisting for 5 days after infiltration, suggesting sustained photosystem efficiency changes. Our work highlights the need to consider the effect of infiltration when performing biological studies and aims to facilitate the optimization of protocols commonly used in plant science and biotechnology.","number":"1","urldate":"2025-01-31","journal":"ACS Agricultural Science & Technology","author":[{"propositions":[],"lastnames":["Routier"],"firstnames":["Cyril"],"suffixes":[]},{"propositions":[],"lastnames":["Hermida-Carrera"],"firstnames":["Carmen"],"suffixes":[]},{"propositions":[],"lastnames":["Stavrinidou"],"firstnames":["Eleni"],"suffixes":[]}],"month":"December","year":"2024","pmid":"39850806","pmcid":"PMC11752493","pages":"28–35","bibtex":"@article{routier_investigating_2024,\n\ttitle = {Investigating the {Effect} of {Syringe} {Infiltration} on {Nicotiana} tabacum ({Tobacco})},\n\tvolume = {5},\n\tissn = {2692-1952},\n\turl = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11752493/},\n\tdoi = {10.1021/acsagscitech.4c00170},\n\tabstract = {, Plant infiltration techniques, particularly agroinfiltration,\nhave\ntransformed plant science and biotechnology by enabling transient\ngene expression for genetic engineering of plants or genomic studies.\nRecently, the use of infiltration has expanded to introduce nanomaterials\nand polymers in plants to enable nonnative functionalities. Despite\nits wide use, the impact of the infiltration process per se on plant physiology needs to be better understood. This study investigates\nthe effect of syringe infiltration, a commonly employed technique\nin plants, using a typical infiltration buffer solution. Noninvasive\nand real-time monitoring methods, including high-resolution thermal\nimaging and a porometer/fluorometer, were used to study the physiological\nresponses and stress levels of the infiltrated plants. Our results\nrevealed localized cell damage at the infiltration site due to syringe\ncompression, but the overall cell viability and tissue integrity were\nlargely unaffected. Thermography showed a temporary temperature increase\nof the leaves and stomatal conductance alterations postinfiltration,\nwith leaf recovery in 3–6 days. Additionally, fluorescence\nmeasurements indicated a 6\\% decrease in maximum quantum efficiency\n(Fv/Fm) and\na 34\\% decrease in photosystem II (ΦPSII) quantum yield, persisting\nfor 5 days after infiltration, suggesting sustained photosystem efficiency\nchanges. Our work highlights the need to consider the effect of infiltration\nwhen performing biological studies and aims to facilitate the optimization\nof protocols commonly used in plant science and biotechnology.},\n\tnumber = {1},\n\turldate = {2025-01-31},\n\tjournal = {ACS Agricultural Science \\& Technology},\n\tauthor = {Routier, Cyril and Hermida-Carrera, Carmen and Stavrinidou, Eleni},\n\tmonth = dec,\n\tyear = {2024},\n\tpmid = {39850806},\n\tpmcid = {PMC11752493},\n\tpages = {28--35},\n}\n\n\n\n\n\n\n\n\n\n\n\n","author_short":["Routier, C.","Hermida-Carrera, C.","Stavrinidou, E."],"key":"routier_investigating_2024","id":"routier_investigating_2024","bibbaseid":"routier-hermidacarrera-stavrinidou-investigatingtheeffectofsyringeinfiltrationonnicotianatabacumtobacco-2024","role":"author","urls":{"Paper":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11752493/"},"metadata":{"authorlinks":{}},"html":""},"bibtype":"article","biburl":"https://bibbase.org/zotero/upscpub","dataSources":["9cGcv2t8pRzC92kzs"],"keywords":[],"search_terms":["investigating","effect","syringe","infiltration","nicotiana","tabacum","tobacco","routier","hermida-carrera","stavrinidou"],"title":"Investigating the Effect of Syringe Infiltration on Nicotiana tabacum (Tobacco)","year":2024}