L’approche métabolomique par spectrométrie de masse : évidences, incertitudes et points critiques. Royer, A., Escourrou, A., Guitton, Y., Dervilly-Pinel, G., Antignac, J., & Bizec, B. L. abstract bibtex Metabolomics is a methodological approach emerged in the late 2 000s in line with other «- omics» : genomics, transcriptomics and proteomics. Like these other approaches, metabolomics is used in various fields and aims at measuring simultaneously all small endogenous metabolites (\textless 1 000 Da) impacted by a disturbance affecting a given biological system. Ultimately, these measures give insights in differences, often tenuous, between different states of a biological system. To achieve with confidence such a degree of sensitivity, it is necessary to master all the different steps of the approach. Metabolomics involves several disciplines (biology, chemistry, bioinformatics, and statistics) and such diversity multiplies the possibilities of confounding factors to arise and thus increases the sources of results misinterpretation. The expertise acquired over the years by researchers from the different areas involved in metabolomics can be briefly summarized in a generic workflow that from the experimental design to the biological interpretation of the data, intends at minimizing all sources of undesirable variability. All this is done in order to respond to this «simple» question : are the differences observed at the end of the process really and only linked to a biological reality ? Here, we discuss the first steps of a metabolomics study: experimental design, sample preparation, data acquisition and pre-processing, with the primary objective to provide clues for a critical assessment of the results and to provide the foundation for the successful implementation of a metabolomics study, in other words, a study meeting the quality criteria for an unbiased interpretation. Examples of illustration mainly refer to studies of mass spectrometric based untargeted metabolomics in the field of chemical food safety.
@article{royer_lapproche_2017,
title = {L’approche métabolomique par spectrométrie de masse : évidences, incertitudes et points critiques},
abstract = {Metabolomics is a methodological approach emerged in the late 2 000s in line with other «- omics» : genomics, transcriptomics and proteomics. Like these other approaches, metabolomics is used in various fields and aims at measuring simultaneously all small endogenous metabolites ({\textless} 1 000 Da) impacted by a disturbance affecting a given biological system. Ultimately, these measures give insights in differences, often tenuous, between different states of a biological system. To achieve with confidence such a degree of sensitivity, it is necessary to master all the different steps of the approach. Metabolomics involves several disciplines (biology, chemistry, bioinformatics, and statistics) and such diversity multiplies the possibilities of confounding factors to arise and thus increases the sources of results misinterpretation. The expertise acquired over the years by researchers from the different areas involved in metabolomics can be briefly summarized in a generic workflow that from the experimental design to the biological interpretation of the data, intends at minimizing all sources of undesirable variability. All this is done in order to respond to this «simple» question : are the differences observed at the end of the process really and only linked to a biological reality ? Here, we discuss the first steps of a metabolomics study: experimental design, sample preparation, data acquisition and pre-processing, with the primary objective to provide clues for a critical assessment of the results and to provide the foundation for the successful implementation of a metabolomics study, in other words, a study meeting the quality criteria for an unbiased interpretation. Examples of illustration mainly refer to studies of mass spectrometric based untargeted metabolomics in the field of chemical food safety.},
pages = {8},
author = {Royer, Anne-Lise and Escourrou, Antoine and Guitton, Yann and Dervilly-Pinel, Gaud and Antignac, Jean-Philippe and Bizec, Bruno {LE}},
date = {2017},
langid = {french},
file = {Royer et al. - 2017 - L’approche métabolomique par spectrométrie de mass.pdf:C\:\\Users\\ygu\\Documents\\PCPOR066_YGU\\YGU\\Zotero\\storage\\Q3A785G6\\Royer et al. - 2017 - L’approche métabolomique par spectrométrie de mass.pdf:application/pdf}
}
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