@article{rueckert_molecular_2010,
	title = {Molecular systematics of marine gregarines ({Apicomplexa}) from {North}-eastern {Pacific} polychaetes and nemerteans, with descriptions of three novel species: \textit{{Lecudina} phyllochaetopteri} sp. nov., \textit{{Difficilina} tubulani }sp. nov. and \textit{{Difficilina} paranemertis} sp. nov.},
	volume = {60},
	issn = {1466-5026, 1466-5034},
	shorttitle = {Molecular systematics of marine gregarines ({Apicomplexa}) from {North}-eastern {Pacific} polychaetes and nemerteans, with descriptions of three novel species},
	url = {https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.016436-0},
	doi = {10.1099/ijs.0.016436-0},
	abstract = {Most eugregarine apicomplexans infecting the intestines of marine invertebrates have been described within the family Lecudinidae and the type genus
              Lecudina
              . The diversity of these parasites is vast and poorly understood and only a tiny number of species has been characterized at the molecular phylogenetic level. DNA sequences coupled with high-resolution micrographs of trophozoites provide an efficient and precise approach for delimiting gregarine lineages from one another and also facilitate our overall understanding of gregarine biodiversity. In this study, phylogenetic analyses of small subunit (SSU) rDNA sequences from five (uncultivated) gregarines isolated from polychaetes and nemerteans in the North-eastern Pacific Ocean are presented.
              Lecudina phyllochaetopteri
              sp. nov. was isolated from the intestines of the parchment tubeworm
              Phyllochaetopterus prolifica
              (Polychaeta).
              Lecudina longissima
              and
              Lecudina polymorpha
              were both isolated from the intestines of
              Lumbrineris japonica
              (Polychaeta).
              Difficilina tubulani
              sp. nov. was isolated from the nemertean
              Tubulanus polymorpha
              and
              Difficilina paranemertis
              sp. nov. was isolated from the nemertean
              Paranemertes peregrina
              . This is the first report of molecular sequence data from gregarines that infect nemerteans. The two novel species of the genus
              Difficilina
              described in this study formed a strongly supported clade in the phylogenetic analyses. This
              Difficilina
              clade formed the sister group to a robust subclade of lecudinids consisting of
              Lecudina longissima
              ,
              Lecudina phyllochaetopteri
              sp. nov. (which lacked epicytic folds),
              Lecudina tuzetae
              , species of the genus
              Lankesteria
              and several sequences derived from previous environmental DNA surveys of marine biodiversity.},
	language = {en},
	number = {11},
	urldate = {2021-07-27},
	journal = {International Journal of Systematic and Evolutionary Microbiology},
	author = {Rueckert, Sonja and Chantangsi, Chitchai and Leander, Brian S.},
	month = nov,
	year = {2010},
	pages = {2681--2690},
}

{"_id":"zgzo26R3pyB4rYFsj","bibbaseid":"rueckert-chantangsi-leander-molecularsystematicsofmarinegregarinesapicomplexafromnortheasternpacificpolychaetesandnemerteanswithdescriptionsofthreenovelspeciesilecudinaphyllochaetopteriispnovidifficilinatubulaniispnovandidifficilinaparanemertisispnov-2010","author_short":["Rueckert, S.","Chantangsi, C.","Leander, B. S."],"bibdata":{"bibtype":"article","type":"article","title":"Molecular systematics of marine gregarines (Apicomplexa) from North-eastern Pacific polychaetes and nemerteans, with descriptions of three novel species: <i>Lecudina phyllochaetopteri</i> sp. nov., <i>Difficilina tubulani </i>sp. nov. and <i>Difficilina paranemertis</i> sp. nov.","volume":"60","issn":"1466-5026, 1466-5034","shorttitle":"Molecular systematics of marine gregarines (Apicomplexa) from North-eastern Pacific polychaetes and nemerteans, with descriptions of three novel species","url":"https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.016436-0","doi":"10.1099/ijs.0.016436-0","abstract":"Most eugregarine apicomplexans infecting the intestines of marine invertebrates have been described within the family Lecudinidae and the type genus Lecudina . The diversity of these parasites is vast and poorly understood and only a tiny number of species has been characterized at the molecular phylogenetic level. DNA sequences coupled with high-resolution micrographs of trophozoites provide an efficient and precise approach for delimiting gregarine lineages from one another and also facilitate our overall understanding of gregarine biodiversity. In this study, phylogenetic analyses of small subunit (SSU) rDNA sequences from five (uncultivated) gregarines isolated from polychaetes and nemerteans in the North-eastern Pacific Ocean are presented. Lecudina phyllochaetopteri sp. nov. was isolated from the intestines of the parchment tubeworm Phyllochaetopterus prolifica (Polychaeta). Lecudina longissima and Lecudina polymorpha were both isolated from the intestines of Lumbrineris japonica (Polychaeta). Difficilina tubulani sp. nov. was isolated from the nemertean Tubulanus polymorpha and Difficilina paranemertis sp. nov. was isolated from the nemertean Paranemertes peregrina . This is the first report of molecular sequence data from gregarines that infect nemerteans. The two novel species of the genus Difficilina described in this study formed a strongly supported clade in the phylogenetic analyses. This Difficilina clade formed the sister group to a robust subclade of lecudinids consisting of Lecudina longissima , Lecudina phyllochaetopteri sp. nov. (which lacked epicytic folds), Lecudina tuzetae , species of the genus Lankesteria and several sequences derived from previous environmental DNA surveys of marine biodiversity.","language":"en","number":"11","urldate":"2021-07-27","journal":"International Journal of Systematic and Evolutionary Microbiology","author":[{"propositions":[],"lastnames":["Rueckert"],"firstnames":["Sonja"],"suffixes":[]},{"propositions":[],"lastnames":["Chantangsi"],"firstnames":["Chitchai"],"suffixes":[]},{"propositions":[],"lastnames":["Leander"],"firstnames":["Brian","S."],"suffixes":[]}],"month":"November","year":"2010","pages":"2681–2690","bibtex":"@article{rueckert_molecular_2010,\n\ttitle = {Molecular systematics of marine gregarines ({Apicomplexa}) from {North}-eastern {Pacific} polychaetes and nemerteans, with descriptions of three novel species: \\textit{{Lecudina} phyllochaetopteri} sp. nov., \\textit{{Difficilina} tubulani }sp. nov. and \\textit{{Difficilina} paranemertis} sp. nov.},\n\tvolume = {60},\n\tissn = {1466-5026, 1466-5034},\n\tshorttitle = {Molecular systematics of marine gregarines ({Apicomplexa}) from {North}-eastern {Pacific} polychaetes and nemerteans, with descriptions of three novel species},\n\turl = {https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.016436-0},\n\tdoi = {10.1099/ijs.0.016436-0},\n\tabstract = {Most eugregarine apicomplexans infecting the intestines of marine invertebrates have been described within the family Lecudinidae and the type genus\n Lecudina\n . The diversity of these parasites is vast and poorly understood and only a tiny number of species has been characterized at the molecular phylogenetic level. DNA sequences coupled with high-resolution micrographs of trophozoites provide an efficient and precise approach for delimiting gregarine lineages from one another and also facilitate our overall understanding of gregarine biodiversity. In this study, phylogenetic analyses of small subunit (SSU) rDNA sequences from five (uncultivated) gregarines isolated from polychaetes and nemerteans in the North-eastern Pacific Ocean are presented.\n Lecudina phyllochaetopteri\n sp. nov. was isolated from the intestines of the parchment tubeworm\n Phyllochaetopterus prolifica\n (Polychaeta).\n Lecudina longissima\n and\n Lecudina polymorpha\n were both isolated from the intestines of\n Lumbrineris japonica\n (Polychaeta).\n Difficilina tubulani\n sp. nov. was isolated from the nemertean\n Tubulanus polymorpha\n and\n Difficilina paranemertis\n sp. nov. was isolated from the nemertean\n Paranemertes peregrina\n . This is the first report of molecular sequence data from gregarines that infect nemerteans. The two novel species of the genus\n Difficilina\n described in this study formed a strongly supported clade in the phylogenetic analyses. This\n Difficilina\n clade formed the sister group to a robust subclade of lecudinids consisting of\n Lecudina longissima\n ,\n Lecudina phyllochaetopteri\n sp. nov. (which lacked epicytic folds),\n Lecudina tuzetae\n , species of the genus\n Lankesteria\n and several sequences derived from previous environmental DNA surveys of marine biodiversity.},\n\tlanguage = {en},\n\tnumber = {11},\n\turldate = {2021-07-27},\n\tjournal = {International Journal of Systematic and Evolutionary Microbiology},\n\tauthor = {Rueckert, Sonja and Chantangsi, Chitchai and Leander, Brian S.},\n\tmonth = nov,\n\tyear = {2010},\n\tpages = {2681--2690},\n}\n\n","author_short":["Rueckert, S.","Chantangsi, C.","Leander, B. S."],"key":"rueckert_molecular_2010","id":"rueckert_molecular_2010","bibbaseid":"rueckert-chantangsi-leander-molecularsystematicsofmarinegregarinesapicomplexafromnortheasternpacificpolychaetesandnemerteanswithdescriptionsofthreenovelspeciesilecudinaphyllochaetopteriispnovidifficilinatubulaniispnovandidifficilinaparanemertisispnov-2010","role":"author","urls":{"Paper":"https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.016436-0"},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://api.zotero.org/users/3476755/collections/4R6FPLUM/items?key=ckhubDoO51X1cQZZ35NkGdxk&format=bibtex&limit=100","dataSources":["sjcnwJbp7RFrMHX8Q"],"keywords":[],"search_terms":["molecular","systematics","marine","gregarines","apicomplexa","north","eastern","pacific","polychaetes","nemerteans","descriptions","three","novel","species","lecudina","phyllochaetopteri","nov","difficilina","tubulani","nov","difficilina","paranemertis","nov","rueckert","chantangsi","leander"],"title":"Molecular systematics of marine gregarines (Apicomplexa) from North-eastern Pacific polychaetes and nemerteans, with descriptions of three novel species: <i>Lecudina phyllochaetopteri</i> sp. nov., <i>Difficilina tubulani </i>sp. nov. and <i>Difficilina paranemertis</i> sp. nov.","year":2010}