High-throughput paraffin section in situ hybridization and dual immunohistochemistry on mouse tissues. Rumballe, B, Georgas, K, & Little, M. CSH Protocols, July, 2008.
doi  abstract   bibtex   
Section in situ hybridization (SISH) is a high-resolution tool used to analyze gene expression patterns. This protocol utilizes the Tecan Freedom EVO150 platform to perform high-throughput SISH on paraffin sections to detect mRNA with a digoxigenin (DIG)-labeled probe. The slide is mounted and imaged before performing immunohistochemistry (IHC) on the same section. The dual reaction enables a marker of protein expression to be localized on the same section as the mRNA and facilitates more accurate annotation of the gene expression.
@article{rumballe_high-throughput_2008,
	title = {High-throughput paraffin section in situ hybridization and dual immunohistochemistry on mouse tissues},
	volume = {2008},
	doi = {10.1101/pdb.prot5030},
	abstract = {Section in situ hybridization (SISH) is a high-resolution tool used to analyze gene expression patterns. This protocol utilizes the Tecan Freedom EVO150 platform to perform high-throughput SISH on paraffin sections to detect mRNA with a digoxigenin (DIG)-labeled probe. The slide is mounted and imaged before performing immunohistochemistry (IHC) on the same section. The dual reaction enables a marker of protein expression to be localized on the same section as the mRNA and facilitates more accurate annotation of the gene expression.},
	journal = {CSH Protocols},
	author = {Rumballe, B and Georgas, K and Little, MH},
	month = jul,
	year = {2008},
}
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