@article{
 title = {A new type of perinuclear anti-neutrophil cytoplasmic antibody (p-ANCA) in active ulcerative colitis but not in Crohn's disease},
 type = {article},
 year = {1990},
 identifiers = {[object Object]},
 keywords = {Adult,Antibodies, Antineutrophil Cytoplasmic,Antibody Specificity,Autoantibodies/*blood,Colitis, Ulcerative/diagnosis/*immunology,Crohn Disease/diagnosis/*immunology,Cytoplasm/immunology,Diagnosis, Differential,Female,Fluorescent Antibody Technique,Humans,Male,Neutrophils/immunology},
 pages = {406-413},
 volume = {181},
 websites = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2099908},
 edition = {1990/11/01},
 id = {83647c23-42ef-3bd7-8047-f51ef5bda066},
 created = {2017-06-19T13:43:59.446Z},
 file_attached = {false},
 profile_id = {de68dde1-2ff3-3a4e-a214-ef424d0c7646},
 group_id = {b2078731-0913-33b9-8902-a53629a24e83},
 last_modified = {2017-06-19T13:43:59.634Z},
 read = {false},
 starred = {false},
 authored = {false},
 confirmed = {true},
 hidden = {false},
 source_type = {Journal Article},
 language = {eng},
 notes = {<m:note>Rump, J A<m:linebreak/>Scholmerich, J<m:linebreak/>Gross, V<m:linebreak/>Roth, M<m:linebreak/>Helfesrieder, R<m:linebreak/>Rautmann, A<m:linebreak/>Ludemann, J<m:linebreak/>Gross, W L<m:linebreak/>Peter, H H<m:linebreak/>Comparative Study<m:linebreak/>Research Support, Non-U.S. Gov't<m:linebreak/>Germany<m:linebreak/>Immunobiology<m:linebreak/>Immunobiology. 1990 Nov;181(4-5):406-13.</m:note>},
 abstract = {Sera of 64 patients with chronic inflammatory bowel disease (IBD) were screened for antibodies against neutrophil cytoplasmic antigens (ANCA) using an indirect immunofluorescence technique on ethanol-fixed human neutrophil granulocytes. 20 of 34 sera (59%) from patients with ulcerative colitis (UC) produced a fine-granular and perinuclear ANCA staining pattern (p-ANCA) clearly different from the typical diffuse and granular cytoplasmic ANCA fluorescence (c-ANCA, synonym ACPA) seen in active Wegener's granulomatosis (WG). The majority of the 20 p-ANCA positive UC patients had a high inflammatory disease activity. Among the 14 p-ANCA negative UC patients nine were without steroids; five of them had active disease, two were inactive and two had previously undergone colectomy. The remaining five patients still had active disease but received steroids for more than 4 weeks. Only 3 of the 30 sera from patients with Crohn's disease (CD) showed positive p-ANCA reactions. To narrow the specificity of the p-ANCA reaction all 64 sera were tested by ELISA for antibodies against anti-proteinase-3 (WG specific) and on HEp-2 cells for antinuclear (ANA) and anticytoplasmic antibodies. Ten p-ANCA positive UC sera were also tested in a myeloperoxidase ELISA. Only one UC serum reacted positively in the proteinase-3-ELISA and another one produced a weakly positive anti-nucleolar ANA fluorescence on HEp-2 cells. None of the tested sera reacted with myeloperoxidase suggesting that the p-ANCA staining pattern of granulocytes is not restricted to anti-myeloperoxidase antibodies as reported in the literature.(ABSTRACT TRUNCATED AT 250 WORDS)},
 bibtype = {article},
 author = {Rump, J A and Scholmerich, J and Gross, V and Roth, M and Helfesrieder, R and Rautmann, A and Ludemann, J and Gross, W L and Peter, H H},
 journal = {Immunobiology},
 number = {4-5}
}

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The majority of the 20 p-ANCA positive UC patients had a high inflammatory disease activity. Among the 14 p-ANCA negative UC patients nine were without steroids; five of them had active disease, two were inactive and two had previously undergone colectomy. The remaining five patients still had active disease but received steroids for more than 4 weeks. Only 3 of the 30 sera from patients with Crohn's disease (CD) showed positive p-ANCA reactions. To narrow the specificity of the p-ANCA reaction all 64 sera were tested by ELISA for antibodies against anti-proteinase-3 (WG specific) and on HEp-2 cells for antinuclear (ANA) and anticytoplasmic antibodies. Ten p-ANCA positive UC sera were also tested in a myeloperoxidase ELISA. Only one UC serum reacted positively in the proteinase-3-ELISA and another one produced a weakly positive anti-nucleolar ANA fluorescence on HEp-2 cells. 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