A new type of perinuclear anti-neutrophil cytoplasmic antibody (p-ANCA) in active ulcerative colitis but not in Crohn's disease. Rump, J., A., Scholmerich, J., Gross, V., Roth, M., Helfesrieder, R., Rautmann, A., Ludemann, J., Gross, W., L., & Peter, H., H. Immunobiology, 181(4-5):406-413, 1990.
Website abstract bibtex Sera of 64 patients with chronic inflammatory bowel disease (IBD) were screened for antibodies against neutrophil cytoplasmic antigens (ANCA) using an indirect immunofluorescence technique on ethanol-fixed human neutrophil granulocytes. 20 of 34 sera (59%) from patients with ulcerative colitis (UC) produced a fine-granular and perinuclear ANCA staining pattern (p-ANCA) clearly different from the typical diffuse and granular cytoplasmic ANCA fluorescence (c-ANCA, synonym ACPA) seen in active Wegener's granulomatosis (WG). The majority of the 20 p-ANCA positive UC patients had a high inflammatory disease activity. Among the 14 p-ANCA negative UC patients nine were without steroids; five of them had active disease, two were inactive and two had previously undergone colectomy. The remaining five patients still had active disease but received steroids for more than 4 weeks. Only 3 of the 30 sera from patients with Crohn's disease (CD) showed positive p-ANCA reactions. To narrow the specificity of the p-ANCA reaction all 64 sera were tested by ELISA for antibodies against anti-proteinase-3 (WG specific) and on HEp-2 cells for antinuclear (ANA) and anticytoplasmic antibodies. Ten p-ANCA positive UC sera were also tested in a myeloperoxidase ELISA. Only one UC serum reacted positively in the proteinase-3-ELISA and another one produced a weakly positive anti-nucleolar ANA fluorescence on HEp-2 cells. None of the tested sera reacted with myeloperoxidase suggesting that the p-ANCA staining pattern of granulocytes is not restricted to anti-myeloperoxidase antibodies as reported in the literature.(ABSTRACT TRUNCATED AT 250 WORDS)
@article{
title = {A new type of perinuclear anti-neutrophil cytoplasmic antibody (p-ANCA) in active ulcerative colitis but not in Crohn's disease},
type = {article},
year = {1990},
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keywords = {Adult,Antibodies, Antineutrophil Cytoplasmic,Antibody Specificity,Autoantibodies/*blood,Colitis, Ulcerative/diagnosis/*immunology,Crohn Disease/diagnosis/*immunology,Cytoplasm/immunology,Diagnosis, Differential,Female,Fluorescent Antibody Technique,Humans,Male,Neutrophils/immunology},
pages = {406-413},
volume = {181},
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edition = {1990/11/01},
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notes = {<m:note>Rump, J A<m:linebreak/>Scholmerich, J<m:linebreak/>Gross, V<m:linebreak/>Roth, M<m:linebreak/>Helfesrieder, R<m:linebreak/>Rautmann, A<m:linebreak/>Ludemann, J<m:linebreak/>Gross, W L<m:linebreak/>Peter, H H<m:linebreak/>Comparative Study<m:linebreak/>Research Support, Non-U.S. Gov't<m:linebreak/>Germany<m:linebreak/>Immunobiology<m:linebreak/>Immunobiology. 1990 Nov;181(4-5):406-13.</m:note>},
abstract = {Sera of 64 patients with chronic inflammatory bowel disease (IBD) were screened for antibodies against neutrophil cytoplasmic antigens (ANCA) using an indirect immunofluorescence technique on ethanol-fixed human neutrophil granulocytes. 20 of 34 sera (59%) from patients with ulcerative colitis (UC) produced a fine-granular and perinuclear ANCA staining pattern (p-ANCA) clearly different from the typical diffuse and granular cytoplasmic ANCA fluorescence (c-ANCA, synonym ACPA) seen in active Wegener's granulomatosis (WG). The majority of the 20 p-ANCA positive UC patients had a high inflammatory disease activity. Among the 14 p-ANCA negative UC patients nine were without steroids; five of them had active disease, two were inactive and two had previously undergone colectomy. The remaining five patients still had active disease but received steroids for more than 4 weeks. Only 3 of the 30 sera from patients with Crohn's disease (CD) showed positive p-ANCA reactions. To narrow the specificity of the p-ANCA reaction all 64 sera were tested by ELISA for antibodies against anti-proteinase-3 (WG specific) and on HEp-2 cells for antinuclear (ANA) and anticytoplasmic antibodies. Ten p-ANCA positive UC sera were also tested in a myeloperoxidase ELISA. Only one UC serum reacted positively in the proteinase-3-ELISA and another one produced a weakly positive anti-nucleolar ANA fluorescence on HEp-2 cells. None of the tested sera reacted with myeloperoxidase suggesting that the p-ANCA staining pattern of granulocytes is not restricted to anti-myeloperoxidase antibodies as reported in the literature.(ABSTRACT TRUNCATED AT 250 WORDS)},
bibtype = {article},
author = {Rump, J A and Scholmerich, J and Gross, V and Roth, M and Helfesrieder, R and Rautmann, A and Ludemann, J and Gross, W L and Peter, H H},
journal = {Immunobiology},
number = {4-5}
}
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