Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Rust, M. J., Bates, M., & Zhuang, X. Nature Methods, 3(10):793--796, October, 2006. Paper doi abstract bibtex We have developed a high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores. In each imaging cycle, only a fraction of the fluorophores were turned on, allowing their positions to be determined with nanometer accuracy. The fluorophore positions obtained from a series of imaging cycles were used to reconstruct the overall image. We demonstrated an imaging resolution of 20 nm. This technique can, in principle, reach molecular-scale resolution.
@article{rust_sub-diffraction-limit_2006,
title = {Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy ({STORM})},
volume = {3},
copyright = {© 2006 Nature Publishing Group},
issn = {1548-7091},
url = {http://www.nature.com/nmeth/journal/v3/n10/full/nmeth929.html},
doi = {10.1038/nmeth929},
abstract = {We have developed a high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores. In each imaging cycle, only a fraction of the fluorophores were turned on, allowing their positions to be determined with nanometer accuracy. The fluorophore positions obtained from a series of imaging cycles were used to reconstruct the overall image. We demonstrated an imaging resolution of 20 nm. This technique can, in principle, reach molecular-scale resolution.},
language = {en},
number = {10},
urldate = {2015-12-14TZ},
journal = {Nature Methods},
author = {Rust, Michael J. and Bates, Mark and Zhuang, Xiaowei},
month = oct,
year = {2006},
pages = {793--796}
}
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