Novel fixed z -direction (FiZD) kidney primordia and an organoid culture system for time-lapse confocal imaging. Saarela, U., Akram, S., U., Desgrange, A., Rak-Raszewska, A., Shan, J., Cereghini, S., Ronkainen, V., Heikkilä, J., Skovorodkin, I., & Vainio, S., J. Development, 144(6):1113-1117, 3, 2017. ![Novel fixed z -direction (FiZD) kidney primordia and an organoid culture system for time-lapse confocal imaging [link]](https://bibbase.org/img/filetypes/link.svg "link")
Website doi abstract bibtex Tissue, organ and organoid cultures provide suitable models for developmental studies, but our understanding of how the organs are assembled at the single-cell level still remains unclear. We describe here a novel fixedz-direction (FiZD) culture setup that permits high-resolution confocal imaging of organoids and embryonic tissues. In a FiZD culture a permeable membrane compresses the tissues onto a glass coverslip and the spacers adjust the thickness, enabling the tissue to grow for up to 12 days. Thus, the kidney rudiment and the organoids can adjust to the limitedz-directional space and yet advance the process of kidney morphogenesis, enabling long-term time-lapse and high-resolution confocal imaging. As the data quality achieved was sufficient for computer-assisted cell segmentation and analysis, the method can be used for studying morphogenesisex vivoat the level of the single constituent cells of a complex mammalian organogenesis model system.
@article{
title = {Novel fixed z -direction (FiZD) kidney primordia and an organoid culture system for time-lapse confocal imaging},
type = {article},
year = {2017},
keywords = {Imaging,Kidney,Organ culture,Organoid,Time-lapse},
pages = {1113-1117},
volume = {144},
websites = {http://dev.biologists.org/lookup/doi/10.1242/dev.142950},
month = {3},
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abstract = {Tissue, organ and organoid cultures provide suitable models for developmental studies, but our understanding of how the organs are assembled at the single-cell level still remains unclear. We describe here a novel fixedz-direction (FiZD) culture setup that permits high-resolution confocal imaging of organoids and embryonic tissues. In a FiZD culture a permeable membrane compresses the tissues onto a glass coverslip and the spacers adjust the thickness, enabling the tissue to grow for up to 12 days. Thus, the kidney rudiment and the organoids can adjust to the limitedz-directional space and yet advance the process of kidney morphogenesis, enabling long-term time-lapse and high-resolution confocal imaging. As the data quality achieved was sufficient for computer-assisted cell segmentation and analysis, the method can be used for studying morphogenesisex vivoat the level of the single constituent cells of a complex mammalian organogenesis model system.},
bibtype = {article},
author = {Saarela, Ulla and Akram, Saad Ullah and Desgrange, Audrey and Rak-Raszewska, Aleksandra and Shan, Jingdong and Cereghini, Silvia and Ronkainen, Veli-Pekka and Heikkilä, Janne and Skovorodkin, Ilya and Vainio, Seppo J},
doi = {10.1242/dev.142950},
journal = {Development},
number = {6}
}
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