@article{
 title = {The transcriptional regulator NtrC controls glucose-6-phosphate dehydrogenase expression and polyhydroxybutyrate synthesis through NADPH availability in Herbaspirillum seropedicae},
 type = {article},
 year = {2017},
 identifiers = {[object Object]},
 keywords = {Qualis2013-2016 A1},
 volume = {7},
 websites = {http://www.nature.com/articles/s41598-017-12649-0},
 month = {12},
 id = {945e2933-1e37-3107-b7d4-a37946a1d334},
 created = {2019-02-04T15:29:28.825Z},
 file_attached = {false},
 profile_id = {81a97cb0-31b8-3385-bd0e-efcfffedaada},
 group_id = {b954b4a7-36ce-39f9-9501-2e3795a979e9},
 last_modified = {2019-02-04T15:29:28.825Z},
 read = {false},
 starred = {false},
 authored = {false},
 confirmed = {true},
 hidden = {false},
 citation_key = {Sacomboio2017},
 source_type = {article},
 language = {en},
 notes = {CAPES qualis 2013-2016 CB I: A1},
 private_publication = {false},
 abstract = {The NTR system is the major regulator of nitrogen metabolism in Bacteria. Despite its broad and well-known role in the assimilation, biosynthesis and recycling of nitrogenous molecules, little is known about its role in carbon metabolism. In this work, we present a new facet of the NTR system in the control of NADPH concentration and the biosynthesis of molecules dependent on reduced coenzyme in Herbaspirillum seropedicae SmR1. We demonstrated that a ntrC mutant strain accumulated high levels of polyhydroxybutyrate (PHB), reaching levels up to 2-fold higher than the parental strain. In the absence of NtrC, the activity of glucose-6-phosphate dehydrogenase (encoded by zwf) increased by 2.8-fold, consequently leading to a 2.1-fold increase in the NADPH/NADP+ ratio. A GFP fusion showed that expression of zwf is likewise controlled by NtrC. The increase in NADPH availability stimulated the production of polyhydroxybutyrate regardless the C/N ratio in the medium. The mutant ntrC was more resistant to H2O2 exposure and controlled the propagation of ROS when facing the oxidative condition, a phenotype associated with the increase in PHB content.},
 bibtype = {article},
 author = {Sacomboio, Euclides Nenga Manuel and Kim, Edson Yu Sin and Correa, Henrique Leonardo Ruchaud and Bonato, Paloma and Pedrosa, Fabio De Oliveira and De Souza, Emanuel Maltempi and Chubatsu, Leda Satie and Müller-Santos, Marcelo},
 journal = {Scientific Reports},
 number = {1}
}

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The mutant ntrC was more resistant to H2O2 exposure and controlled the propagation of ROS when facing the oxidative condition, a phenotype associated with the increase in PHB content.","bibtype":"article","author":"Sacomboio, Euclides Nenga Manuel and Kim, Edson Yu Sin and Correa, Henrique Leonardo Ruchaud and Bonato, Paloma and Pedrosa, Fabio De Oliveira and De Souza, Emanuel Maltempi and Chubatsu, Leda Satie and Müller-Santos, Marcelo","journal":"Scientific Reports","number":"1","bibtex":"@article{\n title = {The transcriptional regulator NtrC controls glucose-6-phosphate dehydrogenase expression and polyhydroxybutyrate synthesis through NADPH availability in Herbaspirillum seropedicae},\n type = {article},\n year = {2017},\n identifiers = {[object Object]},\n keywords = {Qualis2013-2016 A1},\n volume = {7},\n websites = {http://www.nature.com/articles/s41598-017-12649-0},\n month = {12},\n id = {945e2933-1e37-3107-b7d4-a37946a1d334},\n created = {2019-02-04T15:29:28.825Z},\n file_attached = {false},\n profile_id = {81a97cb0-31b8-3385-bd0e-efcfffedaada},\n group_id = {b954b4a7-36ce-39f9-9501-2e3795a979e9},\n last_modified = {2019-02-04T15:29:28.825Z},\n read = {false},\n starred = {false},\n authored = {false},\n confirmed = {true},\n hidden = {false},\n citation_key = {Sacomboio2017},\n source_type = {article},\n language = {en},\n notes = {CAPES qualis 2013-2016 CB I: A1},\n private_publication = {false},\n abstract = {The NTR system is the major regulator of nitrogen metabolism in Bacteria. 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