@article{RN132,
   author = {Salahi, A. and Varhue, W. B. and Farmehini, V. and Hyler, A. R. and Schmelz, E. M. and Davalos, R. V. and Swami, N. S.},
   title = {Self-aligned microfluidic contactless dielectrophoresis device fabricated by single-layer imprinting on cyclic olefin copolymer},
   journal = {Anal Bioanal Chem},
   volume = {412},
   number = {16},
   pages = {3881-3889},
   note = {1618-2650
Salahi, Armita
Varhue, Walter B
Farmehini, Vahid
Hyler, Alexandra R
Schmelz, Eva M
Davalos, Rafael V
Swami, Nathan S
FA2386-18-1-4100/Air Force Office of Scientific Research/
R21 AI130902/AI/NIAID NIH HHS/United States
UL1 TR003015/TR/NCATS NIH HHS/United States
Research Contract/CytoRecovery, Inc./
VBHRC/Virginia Catalyst/
UL1TR003015/TR/NCATS NIH HHS/United States
Journal Article
Germany
2020/05/07
Anal Bioanal Chem. 2020 Jun;412(16):3881-3889. doi: 10.1007/s00216-020-02667-9. Epub 2020 May 5.},
   abstract = {The trapping and deflection of biological cells by dielectrophoresis (DEP) at field non-uniformities in a microfluidic device is often conducted in a contactless dielectrophoresis (cDEP) mode, wherein the electrode channel is in a different layer than the sample channel, so that field penetration through the interceding barrier causes DEP above critical cut-off frequencies. In this manner, through physical separation of the electrode and sample channels, it is possible to spatially modulate electric fields with no electrode-induced damage to biological cells in the sample channel. However, since this device requires interlayer alignment of the electrode to sample channel and needs to maintain a thin interceding barrier (~ 15 μm) over the entire length over which DEP is needed (~ 1 cm), variations in alignment and microstructure fidelity cause wide variations in cDEP trapping level and frequency response across devices. We present a strategy to eliminate interlayer alignment by fabricating self-aligned electrode and sample channels, simultaneously with the interceding barrier layer (14-μm width and 50-μm depth), using a single-layer imprint and bond process on cyclic olefin copolymer. Specifically, by designing support structures, we preserve fidelity of the high aspect ratio insulating posts in the sample channel and the interceding barrier between the sample and electrode channels over the entire device footprint (~ 1 cm). The device operation is validated based on impedance measurements to quantify field penetration through the interceding barrier and by DEP trapping measurements. The presented fabrication strategy can eventually improve cDEP device manufacturing protocols to enable more reproducible DEP performance. Graphical abstract.},
   keywords = {Alkenes/*chemistry
Electrophoresis/*instrumentation
Equipment Design
*Lab-On-A-Chip Devices
Polymers/*chemistry
Dielectrophoresis
Imprint lithography
Microfabrication
Microfluidics
Polymers},
   ISSN = {1618-2642 (Print)
1618-2642},
   DOI = {10.1007/s00216-020-02667-9},
   year = {2020},
   type = {Journal Article}
}

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S."],"bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["Salahi"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Varhue"],"firstnames":["W.","B."],"suffixes":[]},{"propositions":[],"lastnames":["Farmehini"],"firstnames":["V."],"suffixes":[]},{"propositions":[],"lastnames":["Hyler"],"firstnames":["A.","R."],"suffixes":[]},{"propositions":[],"lastnames":["Schmelz"],"firstnames":["E.","M."],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["R.","V."],"suffixes":[]},{"propositions":[],"lastnames":["Swami"],"firstnames":["N.","S."],"suffixes":[]}],"title":"Self-aligned microfluidic contactless dielectrophoresis device fabricated by single-layer imprinting on cyclic olefin copolymer","journal":"Anal Bioanal Chem","volume":"412","number":"16","pages":"3881-3889","note":"1618-2650 Salahi, Armita Varhue, Walter B Farmehini, Vahid Hyler, Alexandra R Schmelz, Eva M Davalos, Rafael V Swami, Nathan S FA2386-18-1-4100/Air Force Office of Scientific Research/ R21 AI130902/AI/NIAID NIH HHS/United States UL1 TR003015/TR/NCATS NIH HHS/United States Research Contract/CytoRecovery, Inc./ VBHRC/Virginia Catalyst/ UL1TR003015/TR/NCATS NIH HHS/United States Journal Article Germany 2020/05/07 Anal Bioanal Chem. 2020 Jun;412(16):3881-3889. doi: 10.1007/s00216-020-02667-9. Epub 2020 May 5.","abstract":"The trapping and deflection of biological cells by dielectrophoresis (DEP) at field non-uniformities in a microfluidic device is often conducted in a contactless dielectrophoresis (cDEP) mode, wherein the electrode channel is in a different layer than the sample channel, so that field penetration through the interceding barrier causes DEP above critical cut-off frequencies. In this manner, through physical separation of the electrode and sample channels, it is possible to spatially modulate electric fields with no electrode-induced damage to biological cells in the sample channel. However, since this device requires interlayer alignment of the electrode to sample channel and needs to maintain a thin interceding barrier (  15 μm) over the entire length over which DEP is needed (  1 cm), variations in alignment and microstructure fidelity cause wide variations in cDEP trapping level and frequency response across devices. We present a strategy to eliminate interlayer alignment by fabricating self-aligned electrode and sample channels, simultaneously with the interceding barrier layer (14-μm width and 50-μm depth), using a single-layer imprint and bond process on cyclic olefin copolymer. Specifically, by designing support structures, we preserve fidelity of the high aspect ratio insulating posts in the sample channel and the interceding barrier between the sample and electrode channels over the entire device footprint (  1 cm). The device operation is validated based on impedance measurements to quantify field penetration through the interceding barrier and by DEP trapping measurements. The presented fabrication strategy can eventually improve cDEP device manufacturing protocols to enable more reproducible DEP performance. Graphical abstract.","keywords":"Alkenes/*chemistry Electrophoresis/*instrumentation Equipment Design *Lab-On-A-Chip Devices Polymers/*chemistry Dielectrophoresis Imprint lithography Microfabrication Microfluidics Polymers","issn":"1618-2642 (Print) 1618-2642","doi":"10.1007/s00216-020-02667-9","year":"2020","bibtex":"@article{RN132,\n author = {Salahi, A. and Varhue, W. B. and Farmehini, V. and Hyler, A. R. and Schmelz, E. M. and Davalos, R. V. and Swami, N. S.},\n title = {Self-aligned microfluidic contactless dielectrophoresis device fabricated by single-layer imprinting on cyclic olefin copolymer},\n journal = {Anal Bioanal Chem},\n volume = {412},\n number = {16},\n pages = {3881-3889},\n note = {1618-2650\nSalahi, Armita\nVarhue, Walter B\nFarmehini, Vahid\nHyler, Alexandra R\nSchmelz, Eva M\nDavalos, Rafael V\nSwami, Nathan S\nFA2386-18-1-4100/Air Force Office of Scientific Research/\nR21 AI130902/AI/NIAID NIH HHS/United States\nUL1 TR003015/TR/NCATS NIH HHS/United States\nResearch Contract/CytoRecovery, Inc./\nVBHRC/Virginia Catalyst/\nUL1TR003015/TR/NCATS NIH HHS/United States\nJournal Article\nGermany\n2020/05/07\nAnal Bioanal Chem. 2020 Jun;412(16):3881-3889. doi: 10.1007/s00216-020-02667-9. Epub 2020 May 5.},\n abstract = {The trapping and deflection of biological cells by dielectrophoresis (DEP) at field non-uniformities in a microfluidic device is often conducted in a contactless dielectrophoresis (cDEP) mode, wherein the electrode channel is in a different layer than the sample channel, so that field penetration through the interceding barrier causes DEP above critical cut-off frequencies. In this manner, through physical separation of the electrode and sample channels, it is possible to spatially modulate electric fields with no electrode-induced damage to biological cells in the sample channel. However, since this device requires interlayer alignment of the electrode to sample channel and needs to maintain a thin interceding barrier (~ 15 μm) over the entire length over which DEP is needed (~ 1 cm), variations in alignment and microstructure fidelity cause wide variations in cDEP trapping level and frequency response across devices. We present a strategy to eliminate interlayer alignment by fabricating self-aligned electrode and sample channels, simultaneously with the interceding barrier layer (14-μm width and 50-μm depth), using a single-layer imprint and bond process on cyclic olefin copolymer. Specifically, by designing support structures, we preserve fidelity of the high aspect ratio insulating posts in the sample channel and the interceding barrier between the sample and electrode channels over the entire device footprint (~ 1 cm). The device operation is validated based on impedance measurements to quantify field penetration through the interceding barrier and by DEP trapping measurements. The presented fabrication strategy can eventually improve cDEP device manufacturing protocols to enable more reproducible DEP performance. Graphical abstract.},\n keywords = {Alkenes/*chemistry\nElectrophoresis/*instrumentation\nEquipment Design\n*Lab-On-A-Chip Devices\nPolymers/*chemistry\nDielectrophoresis\nImprint lithography\nMicrofabrication\nMicrofluidics\nPolymers},\n ISSN = {1618-2642 (Print)\n1618-2642},\n DOI = {10.1007/s00216-020-02667-9},\n year = {2020},\n type = {Journal Article}\n}\n\n","author_short":["Salahi, A.","Varhue, W. B.","Farmehini, V.","Hyler, A. R.","Schmelz, E. M.","Davalos, R. V.","Swami, N. S."],"key":"RN132","id":"RN132","bibbaseid":"salahi-varhue-farmehini-hyler-schmelz-davalos-swami-selfalignedmicrofluidiccontactlessdielectrophoresisdevicefabricatedbysinglelayerimprintingoncyclicolefincopolymer-2020","role":"author","urls":{},"keyword":["Alkenes/*chemistry Electrophoresis/*instrumentation Equipment Design *Lab-On-A-Chip Devices Polymers/*chemistry Dielectrophoresis Imprint lithography Microfabrication Microfluidics Polymers"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/network/files/bdNBTZRXTsoHCgpbh","dataSources":["D4zENc4BfFNBwSYYJ","ZPLjameRikygaiM9B","3XfNmZkLe6o8CvECW","fJQsxtBoqymHQG6tL","LzxgEApraxMPkLTMn","Z2THpXfLYEJf3CB8p"],"keywords":["alkenes/*chemistry electrophoresis/*instrumentation equipment design *lab-on-a-chip devices polymers/*chemistry dielectrophoresis imprint lithography microfabrication microfluidics polymers"],"search_terms":["self","aligned","microfluidic","contactless","dielectrophoresis","device","fabricated","single","layer","imprinting","cyclic","olefin","copolymer","salahi","varhue","farmehini","hyler","schmelz","davalos","swami"],"title":"Self-aligned microfluidic contactless dielectrophoresis device fabricated by single-layer imprinting on cyclic olefin copolymer","year":2020}