@article{RN203,
   author = {Salmanzadeh, A. and Elvington, E. S. and Roberts, P. C. and Schmelz, E. M. and Davalos, R. V.},
   title = {Sphingolipid metabolites modulate dielectric characteristics of cells in a mouse ovarian cancer progression model},
   journal = {Integr Biol (Camb)},
   volume = {5},
   number = {6},
   pages = {843-52},
   note = {1757-9708
Salmanzadeh, Alireza
Elvington, Elizabeth S
Roberts, Paul C
Schmelz, Eva M
Davalos, Rafael V
R21 CA173092/CA/NCI NIH HHS/United States
R01 CA118846/CA/NCI NIH HHS/United States
1R21 CA173092-01/CA/NCI NIH HHS/United States
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
England
2013/04/24
Integr Biol (Camb). 2013 Jun;5(6):843-52. doi: 10.1039/c3ib00008g.},
   abstract = {Currently, conventional cancer treatment regimens often rely upon highly toxic chemotherapeutics or target oncogenes that are variably expressed within the heterogeneous cell population of tumors. These challenges highlight the need for novel treatment strategies that (1) are non-toxic yet able to at least partially reverse the aggressive phenotype of the disease to a benign or very slow-growing state, and (2) act on the cells independently of variably expressed biomarkers. Using a label-independent rapid microfluidic cell manipulation strategy known as contactless dielectrophoresis (cDEP), we investigated the effect of non-toxic concentrations of two bioactive sphingolipid metabolites, sphingosine (So), with potential anti-tumor properties, and sphingosine-1-phosphate (S1P), a tumor-promoting metabolite, on the intrinsic electrical properties of early and late stages of mouse ovarian surface epithelial (MOSE) cancer cells. Previously, we demonstrated that electrical properties change as cells progress from a benign early stage to late malignant stages. Here, we demonstrate an association between So treatment and a shift in the bioelectrical characteristics of late stage MOSE (MOSE-L) cells towards a profile similar to that of benign MOSE-E cells. Particularly, the specific membrane capacitance of MOSE-L cells shifted toward that of MOSE-E cells, decreasing from 23.94 ± 2.75 to 16.46 ± 0.62 mF m(-2) after So treatment, associated with a decrease in membrane protrusions. In contrast, S1P did not reverse the electrical properties of MOSE-L cells. This work is the first to indicate that treatment with non-toxic doses of So correlates with changes in the electrical properties and surface roughness of cells. It also demonstrates the potential of cDEP to be used as a new, rapid technique for drug efficacy studies, and for eventually designing more personalized treatment regimens.},
   keywords = {Animals
Carcinoma, Ovarian Epithelial
Cell Line, Tumor
Disease Progression
Electrophoresis
Female
Lysophospholipids/*metabolism
Mice
Microfluidics
*Models, Biological
Neoplasms, Glandular and Epithelial/*metabolism/*pathology
Ovarian Neoplasms/*metabolism/*pathology
Phenotype
Sphingosine/*analogs & derivatives/*metabolism},
   ISSN = {1757-9694 (Print)
1757-9694},
   DOI = {10.1039/c3ib00008g},
   year = {2013},
   type = {Journal Article}
}

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V."],"bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["Salmanzadeh"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Elvington"],"firstnames":["E.","S."],"suffixes":[]},{"propositions":[],"lastnames":["Roberts"],"firstnames":["P.","C."],"suffixes":[]},{"propositions":[],"lastnames":["Schmelz"],"firstnames":["E.","M."],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["R.","V."],"suffixes":[]}],"title":"Sphingolipid metabolites modulate dielectric characteristics of cells in a mouse ovarian cancer progression model","journal":"Integr Biol (Camb)","volume":"5","number":"6","pages":"843-52","note":"1757-9708 Salmanzadeh, Alireza Elvington, Elizabeth S Roberts, Paul C Schmelz, Eva M Davalos, Rafael V R21 CA173092/CA/NCI NIH HHS/United States R01 CA118846/CA/NCI NIH HHS/United States 1R21 CA173092-01/CA/NCI NIH HHS/United States Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2013/04/24 Integr Biol (Camb). 2013 Jun;5(6):843-52. doi: 10.1039/c3ib00008g.","abstract":"Currently, conventional cancer treatment regimens often rely upon highly toxic chemotherapeutics or target oncogenes that are variably expressed within the heterogeneous cell population of tumors. These challenges highlight the need for novel treatment strategies that (1) are non-toxic yet able to at least partially reverse the aggressive phenotype of the disease to a benign or very slow-growing state, and (2) act on the cells independently of variably expressed biomarkers. Using a label-independent rapid microfluidic cell manipulation strategy known as contactless dielectrophoresis (cDEP), we investigated the effect of non-toxic concentrations of two bioactive sphingolipid metabolites, sphingosine (So), with potential anti-tumor properties, and sphingosine-1-phosphate (S1P), a tumor-promoting metabolite, on the intrinsic electrical properties of early and late stages of mouse ovarian surface epithelial (MOSE) cancer cells. Previously, we demonstrated that electrical properties change as cells progress from a benign early stage to late malignant stages. Here, we demonstrate an association between So treatment and a shift in the bioelectrical characteristics of late stage MOSE (MOSE-L) cells towards a profile similar to that of benign MOSE-E cells. Particularly, the specific membrane capacitance of MOSE-L cells shifted toward that of MOSE-E cells, decreasing from 23.94 ± 2.75 to 16.46 ± 0.62 mF m(-2) after So treatment, associated with a decrease in membrane protrusions. In contrast, S1P did not reverse the electrical properties of MOSE-L cells. This work is the first to indicate that treatment with non-toxic doses of So correlates with changes in the electrical properties and surface roughness of cells. It also demonstrates the potential of cDEP to be used as a new, rapid technique for drug efficacy studies, and for eventually designing more personalized treatment regimens.","keywords":"Animals Carcinoma, Ovarian Epithelial Cell Line, Tumor Disease Progression Electrophoresis Female Lysophospholipids/*metabolism Mice Microfluidics *Models, Biological Neoplasms, Glandular and Epithelial/*metabolism/*pathology Ovarian Neoplasms/*metabolism/*pathology Phenotype Sphingosine/*analogs & derivatives/*metabolism","issn":"1757-9694 (Print) 1757-9694","doi":"10.1039/c3ib00008g","year":"2013","bibtex":"@article{RN203,\n author = {Salmanzadeh, A. and Elvington, E. S. and Roberts, P. C. and Schmelz, E. M. and Davalos, R. V.},\n title = {Sphingolipid metabolites modulate dielectric characteristics of cells in a mouse ovarian cancer progression model},\n journal = {Integr Biol (Camb)},\n volume = {5},\n number = {6},\n pages = {843-52},\n note = {1757-9708\nSalmanzadeh, Alireza\nElvington, Elizabeth S\nRoberts, Paul C\nSchmelz, Eva M\nDavalos, Rafael V\nR21 CA173092/CA/NCI NIH HHS/United States\nR01 CA118846/CA/NCI NIH HHS/United States\n1R21 CA173092-01/CA/NCI NIH HHS/United States\nJournal Article\nResearch Support, N.I.H., Extramural\nResearch Support, Non-U.S. Gov't\nResearch Support, U.S. Gov't, Non-P.H.S.\nEngland\n2013/04/24\nIntegr Biol (Camb). 2013 Jun;5(6):843-52. doi: 10.1039/c3ib00008g.},\n abstract = {Currently, conventional cancer treatment regimens often rely upon highly toxic chemotherapeutics or target oncogenes that are variably expressed within the heterogeneous cell population of tumors. These challenges highlight the need for novel treatment strategies that (1) are non-toxic yet able to at least partially reverse the aggressive phenotype of the disease to a benign or very slow-growing state, and (2) act on the cells independently of variably expressed biomarkers. Using a label-independent rapid microfluidic cell manipulation strategy known as contactless dielectrophoresis (cDEP), we investigated the effect of non-toxic concentrations of two bioactive sphingolipid metabolites, sphingosine (So), with potential anti-tumor properties, and sphingosine-1-phosphate (S1P), a tumor-promoting metabolite, on the intrinsic electrical properties of early and late stages of mouse ovarian surface epithelial (MOSE) cancer cells. Previously, we demonstrated that electrical properties change as cells progress from a benign early stage to late malignant stages. Here, we demonstrate an association between So treatment and a shift in the bioelectrical characteristics of late stage MOSE (MOSE-L) cells towards a profile similar to that of benign MOSE-E cells. Particularly, the specific membrane capacitance of MOSE-L cells shifted toward that of MOSE-E cells, decreasing from 23.94 ± 2.75 to 16.46 ± 0.62 mF m(-2) after So treatment, associated with a decrease in membrane protrusions. In contrast, S1P did not reverse the electrical properties of MOSE-L cells. This work is the first to indicate that treatment with non-toxic doses of So correlates with changes in the electrical properties and surface roughness of cells. It also demonstrates the potential of cDEP to be used as a new, rapid technique for drug efficacy studies, and for eventually designing more personalized treatment regimens.},\n keywords = {Animals\nCarcinoma, Ovarian Epithelial\nCell Line, Tumor\nDisease Progression\nElectrophoresis\nFemale\nLysophospholipids/*metabolism\nMice\nMicrofluidics\n*Models, Biological\nNeoplasms, Glandular and Epithelial/*metabolism/*pathology\nOvarian Neoplasms/*metabolism/*pathology\nPhenotype\nSphingosine/*analogs & derivatives/*metabolism},\n ISSN = {1757-9694 (Print)\n1757-9694},\n DOI = {10.1039/c3ib00008g},\n year = {2013},\n type = {Journal Article}\n}\n\n","author_short":["Salmanzadeh, A.","Elvington, E. S.","Roberts, P. C.","Schmelz, E. M.","Davalos, R. 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