Isolation of prostate tumor initiating cells (TICs) through their dielectrophoretic signature. Salmanzadeh, A., Romero, L., Shafiee, H., Gallo-Villanueva, R. C., Stremler, M. A., Cramer, S. D., & Davalos, R. V. Lab Chip, 12(1):182-9, 2012. 1473-0189 Salmanzadeh, Alireza Romero, Lina Shafiee, Hadi Gallo-Villanueva, Roberto C Stremler, Mark A Cramer, Scott D Davalos, Rafael V Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2011/11/10 Lab Chip. 2012 Jan 7;12(1):182-9. doi: 10.1039/c1lc20701f. Epub 2011 Nov 9.
doi  abstract   bibtex   
In this study, the dielectrophoretic response of prostate tumor initiating cells (TICs) was investigated in a microfluidic system utilizing contactless dielectrophoresis (cDEP). The dielectrophoretic response of prostate TICs was observed to be distinctively different than that for non-TICs, enabling them to be sorted using cDEP. Culturing the sorted TICs generated spheroids, indicating that they were indeed initiating cells. This study presents the first marker-free TIC separation from non-TICs utilizing their electrical fingerprints through dielectrophoresis.
@article{RN217,
   author = {Salmanzadeh, A. and Romero, L. and Shafiee, H. and Gallo-Villanueva, R. C. and Stremler, M. A. and Cramer, S. D. and Davalos, R. V.},
   title = {Isolation of prostate tumor initiating cells (TICs) through their dielectrophoretic signature},
   journal = {Lab Chip},
   volume = {12},
   number = {1},
   pages = {182-9},
   note = {1473-0189
Salmanzadeh, Alireza
Romero, Lina
Shafiee, Hadi
Gallo-Villanueva, Roberto C
Stremler, Mark A
Cramer, Scott D
Davalos, Rafael V
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
England
2011/11/10
Lab Chip. 2012 Jan 7;12(1):182-9. doi: 10.1039/c1lc20701f. Epub 2011 Nov 9.},
   abstract = {In this study, the dielectrophoretic response of prostate tumor initiating cells (TICs) was investigated in a microfluidic system utilizing contactless dielectrophoresis (cDEP). The dielectrophoretic response of prostate TICs was observed to be distinctively different than that for non-TICs, enabling them to be sorted using cDEP. Culturing the sorted TICs generated spheroids, indicating that they were indeed initiating cells. This study presents the first marker-free TIC separation from non-TICs utilizing their electrical fingerprints through dielectrophoresis.},
   keywords = {Cell Separation/*instrumentation/methods
Cell Survival
Computer Simulation
Electrophoresis/*instrumentation
Flow Cytometry
Humans
Male
Microfluidic Analytical Techniques/*instrumentation
Neoplastic Stem Cells/*chemistry/pathology
Prostatic Neoplasms/chemistry/*pathology
Reproducibility of Results
Spheroids, Cellular/chemistry/cytology
Tumor Cells, Cultured},
   ISSN = {1473-0189},
   DOI = {10.1039/c1lc20701f},
   year = {2012},
   type = {Journal Article}
}

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