Isolation of rare cancer cells from blood cells using dielectrophoresis. Salmanzadeh, A., Sano, M. B., Shafiee, H., Stremler, M. A., & Davalos, R. V. Annu Int Conf IEEE Eng Med Biol Soc, 2012:590-3, 2012. 2694-0604 Salmanzadeh, Alireza Sano, Michael B Shafiee, Hadi Stremler, Mark A Davalos, Rafael V Evaluation Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. United States 2013/02/01 Annu Int Conf IEEE Eng Med Biol Soc. 2012;2012:590-3. doi: 10.1109/EMBC.2012.6346000.
doi  abstract   bibtex   
In this study, we investigate the application of contactless dielectrophoresis (cDEP) for isolating cancer cells from blood cells. Devices with throughput of 0.2 mL/hr (equivalent to sorting 3×10(6) cells per minute) were used to trap breast cancer cells while allowing blood cells through. We have shown that this technique is able to isolate cancer cells in concentration as low as 1 cancer cell per 10(6) hematologic cells (equivalent to 1000 cancer cells in 1 mL of blood). We achieved 96% trapping of the cancer cells at 600 kHz and 300 V(RMS).
@article{RN205,
   author = {Salmanzadeh, A. and Sano, M. B. and Shafiee, H. and Stremler, M. A. and Davalos, R. V.},
   title = {Isolation of rare cancer cells from blood cells using dielectrophoresis},
   journal = {Annu Int Conf IEEE Eng Med Biol Soc},
   volume = {2012},
   pages = {590-3},
   note = {2694-0604
Salmanzadeh, Alireza
Sano, Michael B
Shafiee, Hadi
Stremler, Mark A
Davalos, Rafael V
Evaluation Study
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.
United States
2013/02/01
Annu Int Conf IEEE Eng Med Biol Soc. 2012;2012:590-3. doi: 10.1109/EMBC.2012.6346000.},
   abstract = {In this study, we investigate the application of contactless dielectrophoresis (cDEP) for isolating cancer cells from blood cells. Devices with throughput of 0.2 mL/hr (equivalent to sorting 3×10(6) cells per minute) were used to trap breast cancer cells while allowing blood cells through. We have shown that this technique is able to isolate cancer cells in concentration as low as 1 cancer cell per 10(6) hematologic cells (equivalent to 1000 cancer cells in 1 mL of blood). We achieved 96% trapping of the cancer cells at 600 kHz and 300 V(RMS).},
   keywords = {Blood Cells/pathology
Breast Neoplasms/blood/pathology
Cell Line, Tumor
Cell Separation/instrumentation/*methods
Electrodes
Electrophoresis/instrumentation/*methods
Equipment Design
Female
Humans
Microfluidic Analytical Techniques/instrumentation/methods
Neoplastic Cells, Circulating/*pathology},
   ISSN = {2375-7477},
   DOI = {10.1109/embc.2012.6346000},
   year = {2012},
   type = {Journal Article}
}

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