Organization of core spliceosomal components U5 snRNA loop I and U4/U6 Di-snRNP within U4/U6.U5 Tri-snRNP as revealed by electron cryomicroscopy. Sander, B., Golas, M., M., Makarov, E., M., Brahms, H., Kastner, B., Lührmann, R., & Stark, H. Molecular cell, 24(2):267-78, 10, 2006. ![Organization of core spliceosomal components U5 snRNA loop I and U4/U6 Di-snRNP within U4/U6.U5 Tri-snRNP as revealed by electron cryomicroscopy. [pdf]](https://bibbase.org/img/filetypes/pdf.svg "pdf")
Paper ![Organization of core spliceosomal components U5 snRNA loop I and U4/U6 Di-snRNP within U4/U6.U5 Tri-snRNP as revealed by electron cryomicroscopy. [link]](https://bibbase.org/img/filetypes/link.svg "link")
Website abstract bibtex In eukaryotes, pre-mRNA exons are interrupted by large noncoding introns. Alternative selection of exons and nucleotide-exact removal of introns are performed by the spliceosome, a highly dynamic macromolecular machine. U4/U6.U5 tri-snRNP is the largest and most conserved building block of the spliceosome. By 3D electron cryomicroscopy and labeling, the exon-aligning U5 snRNA loop I is localized at the center of the tetrahedrally shaped tri-snRNP reconstructed to approximately 2.1 nm resolution in vitrified ice. Independent 3D reconstructions of its subunits, U4/U6 and U5 snRNPs, show how U4/U6 and U5 combine to form tri-snRNP and, together with labeling experiments, indicate a close proximity of the spliceosomal core components U5 snRNA loop I and U4/U6 at the center of tri-snRNP. We suggest that this central tri-snRNP region may be the site to which the prespliceosomal U2 snRNA has to approach closely during formation of the catalytic core of the spliceosome.
@article{
title = {Organization of core spliceosomal components U5 snRNA loop I and U4/U6 Di-snRNP within U4/U6.U5 Tri-snRNP as revealed by electron cryomicroscopy.},
type = {article},
year = {2006},
identifiers = {[object Object]},
keywords = {Base Sequence,Catalytic Domain,Cryoelectron Microscopy,Cryoelectron Microscopy: methods,Exons,Hela Cells,Humans,Imaging, Three-Dimensional,Models, Molecular,Molecular Sequence Data,Nucleic Acid Conformation,Protein Conformation,RNA, Small Nuclear,RNA, Small Nuclear: chemistry,Spliceosomes,Spliceosomes: chemistry,Spliceosomes: ultrastructure},
pages = {267-78},
volume = {24},
websites = {http://www.ncbi.nlm.nih.gov/pubmed/17052460},
month = {10},
day = {20},
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abstract = {In eukaryotes, pre-mRNA exons are interrupted by large noncoding introns. Alternative selection of exons and nucleotide-exact removal of introns are performed by the spliceosome, a highly dynamic macromolecular machine. U4/U6.U5 tri-snRNP is the largest and most conserved building block of the spliceosome. By 3D electron cryomicroscopy and labeling, the exon-aligning U5 snRNA loop I is localized at the center of the tetrahedrally shaped tri-snRNP reconstructed to approximately 2.1 nm resolution in vitrified ice. Independent 3D reconstructions of its subunits, U4/U6 and U5 snRNPs, show how U4/U6 and U5 combine to form tri-snRNP and, together with labeling experiments, indicate a close proximity of the spliceosomal core components U5 snRNA loop I and U4/U6 at the center of tri-snRNP. We suggest that this central tri-snRNP region may be the site to which the prespliceosomal U2 snRNA has to approach closely during formation of the catalytic core of the spliceosome.},
bibtype = {article},
author = {Sander, Bjoern and Golas, Monika M and Makarov, Evgeny M and Brahms, Hero and Kastner, Berthold and Lührmann, Reinhard and Stark, Holger},
journal = {Molecular cell},
number = {2}
}