Nanoparticle-Based Discrimination of Single-Nucleotide Polymorphism in Long DNA Sequences. Sanromán-Iglesias, M., Lawrie, C. H., Liz-Marzán, L. M., & Grzelczak, M. Bioconjugate Chemistry, 28(4):903–906, February, 2017. 00005Paper doi abstract bibtex Circulating DNA (ctDNA) and specifically the detection cancer-associated mutations in liquid biopsies promises to revolutionize cancer detection. The main difficulty however is that the length of typical ctDNA fragments (∼150 bases) can form secondary structures potentially obscuring the mutated fragment from detection. We show that an assay based on gold nanoparticles (65 nm) stabilized with DNA (Au@DNA) can discriminate single nucleotide polymorphism in clinically relevant ssDNA sequences (70–140 bases). The preincubation step was crucial to this process, allowing sequential bridging of Au@DNA, so that single base mutation can be discriminated, down to 100 pM concentration.
@article{sanroman-iglesias_nanoparticle-based_2017,
title = {Nanoparticle-{Based} {Discrimination} of {Single}-{Nucleotide} {Polymorphism} in {Long} {DNA} {Sequences}},
volume = {28},
copyright = {1},
issn = {1043-1802},
url = {http://dx.doi.org/10.1021/acs.bioconjchem.7b00028},
doi = {10.1021/acs.bioconjchem.7b00028},
abstract = {Circulating DNA (ctDNA) and specifically the detection cancer-associated mutations in liquid biopsies promises to revolutionize cancer detection. The main difficulty however is that the length of typical ctDNA fragments (∼150 bases) can form secondary structures potentially obscuring the mutated fragment from detection. We show that an assay based on gold nanoparticles (65 nm) stabilized with DNA (Au@DNA) can discriminate single nucleotide polymorphism in clinically relevant ssDNA sequences (70–140 bases). The preincubation step was crucial to this process, allowing sequential bridging of Au@DNA, so that single base mutation can be discriminated, down to 100 pM concentration.},
number = {4},
urldate = {2017-03-01},
journal = {Bioconjugate Chemistry},
author = {Sanromán-Iglesias, María and Lawrie, Charles H. and Liz-Marzán, Luis M. and Grzelczak, Marek},
month = feb,
year = {2017},
note = {00005},
keywords = {biomaGUNE},
pages = {903--906},
}
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