@article{sanroman-iglesias_nanoparticle-based_2017,
	title = {Nanoparticle-{Based} {Discrimination} of {Single}-{Nucleotide} {Polymorphism} in {Long} {DNA} {Sequences}},
	volume = {28},
	copyright = {1},
	issn = {1043-1802},
	url = {http://dx.doi.org/10.1021/acs.bioconjchem.7b00028},
	doi = {10.1021/acs.bioconjchem.7b00028},
	abstract = {Circulating DNA (ctDNA) and specifically the detection cancer-associated mutations in liquid biopsies promises to revolutionize cancer detection. The main difficulty however is that the length of typical ctDNA fragments (∼150 bases) can form secondary structures potentially obscuring the mutated fragment from detection. We show that an assay based on gold nanoparticles (65 nm) stabilized with DNA (Au@DNA) can discriminate single nucleotide polymorphism in clinically relevant ssDNA sequences (70–140 bases). The preincubation step was crucial to this process, allowing sequential bridging of Au@DNA, so that single base mutation can be discriminated, down to 100 pM concentration.},
	number = {4},
	urldate = {2017-03-01},
	journal = {Bioconjugate Chemistry},
	author = {Sanromán-Iglesias, María and Lawrie, Charles H. and Liz-Marzán, Luis M. and Grzelczak, Marek},
	month = feb,
	year = {2017},
	note = {00005},
	keywords = {biomaGUNE},
	pages = {903--906},
}

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