@article{
 title = {Wheat colonization by an Azospirillum brasilense ammonium-excreting strain reveals upregulation of nitrogenase and superior plant growth promotion},
 type = {article},
 year = {2017},
 identifiers = {[object Object]},
 keywords = {Ammonium-excreting mutant,Azospirillum brasilense,Biofertilizer,Glutamine synthetase,glnA gene},
 pages = {245-255},
 volume = {415},
 websites = {http://link.springer.com/10.1007/s11104-016-3140-6},
 month = {6},
 id = {dc7fc732-0913-36b9-b741-f17fbc42813f},
 created = {2019-02-04T15:29:24.682Z},
 file_attached = {false},
 profile_id = {81a97cb0-31b8-3385-bd0e-efcfffedaada},
 group_id = {b954b4a7-36ce-39f9-9501-2e3795a979e9},
 last_modified = {2019-02-04T15:29:24.682Z},
 read = {false},
 starred = {false},
 authored = {false},
 confirmed = {true},
 hidden = {false},
 citation_key = {Santos2017a},
 source_type = {article},
 language = {en},
 notes = {CAPES qualis 2013-2016 CB I: B1},
 private_publication = {false},
 abstract = {© 2016, Springer International Publishing Switzerland. Aims: In this work, an ammonium-excreting strain (HM053) of A. brasilense was further characterized genetically and biochemically, and its abilities to colonize and promote wheat growth were determined. Methods: Immunoblot, reverse transcription-qPCR, and DNA sequencing were used for HM053 characterization. To analyze wheat-A. brasilense interaction nifH::gusA fusions in the wild-type FP2 (FP2-7) and HM053 (HM053-36) backgrounds were employed. Results: HM053 glutamine synthetase (GS) was not adenylylated in response to an ammonium shock or under any condition tested. Sequencing of the glnA gene revealed a substitution of a proline residue by a leucine at position 347 of the GS. Under axenic growth condition, HM053 was capable of colonizing the surface of wheat roots and increased by 30 and 49% the shoot and root dry weight, respectively, when compared with uninoculated plants, and by 30 and 31% when compared with the parental strain FP2. Although HM053-36 and FP2-7 showed GUS activity located mainly at lateral root emergence points, HM053-36 consistently showed stronger signals and expressed the nifH gene at a level 278 fold higher than strain FP2 in planta, according to qPCR data. Conclusions: HM053, a spontaneous mutant in GS, increased wheat root and shoot dry weight when compared to the wild-type FP2. HM053 ability to excrete ammonium and fix nitrogen constitutively, even in the presence of high NH 4 + concentration, could explain why this mutant has a higher potential to promote plant growth than FP2 and suggests HM053 as a potential nitrogen biofertilizer. However, HM053 should be tested under field conditions to evaluate its abilities to compete with indigenous microflora.},
 bibtype = {article},
 author = {Santos, K. F.D.N. and Moure, V. R. and Hauer, V. and Santos, A. R.S. and Donatti, L. and Galvão, C. W. and Pedrosa, F. O. and Souza, E. M. and Wassem, R. and Steffens, M. B.R.},
 journal = {Plant and Soil},
 number = {1-2}
}

{"_id":"okQY6rTKw25225ezR","bibbaseid":"santos-moure-hauer-santos-donatti-galvo-pedrosa-souza-etal-wheatcolonizationbyanazospirillumbrasilenseammoniumexcretingstrainrevealsupregulationofnitrogenaseandsuperiorplantgrowthpromotion-2017","authorIDs":[],"author_short":["Santos, K., F.","Moure, V., R.","Hauer, V.","Santos, A., R.","Donatti, L.","Galvão, C., W.","Pedrosa, F., O.","Souza, E., M.","Wassem, R.","Steffens, M., B."],"bibdata":{"title":"Wheat colonization by an Azospirillum brasilense ammonium-excreting strain reveals upregulation of nitrogenase and superior plant growth promotion","type":"article","year":"2017","identifiers":"[object Object]","keywords":"Ammonium-excreting mutant,Azospirillum brasilense,Biofertilizer,Glutamine synthetase,glnA gene","pages":"245-255","volume":"415","websites":"http://link.springer.com/10.1007/s11104-016-3140-6","month":"6","id":"dc7fc732-0913-36b9-b741-f17fbc42813f","created":"2019-02-04T15:29:24.682Z","file_attached":false,"profile_id":"81a97cb0-31b8-3385-bd0e-efcfffedaada","group_id":"b954b4a7-36ce-39f9-9501-2e3795a979e9","last_modified":"2019-02-04T15:29:24.682Z","read":false,"starred":false,"authored":false,"confirmed":"true","hidden":false,"citation_key":"Santos2017a","source_type":"article","language":"en","notes":"CAPES qualis 2013-2016 CB I: B1","private_publication":false,"abstract":"© 2016, Springer International Publishing Switzerland. Aims: In this work, an ammonium-excreting strain (HM053) of A. brasilense was further characterized genetically and biochemically, and its abilities to colonize and promote wheat growth were determined. Methods: Immunoblot, reverse transcription-qPCR, and DNA sequencing were used for HM053 characterization. To analyze wheat-A. brasilense interaction nifH::gusA fusions in the wild-type FP2 (FP2-7) and HM053 (HM053-36) backgrounds were employed. Results: HM053 glutamine synthetase (GS) was not adenylylated in response to an ammonium shock or under any condition tested. Sequencing of the glnA gene revealed a substitution of a proline residue by a leucine at position 347 of the GS. Under axenic growth condition, HM053 was capable of colonizing the surface of wheat roots and increased by 30 and 49% the shoot and root dry weight, respectively, when compared with uninoculated plants, and by 30 and 31% when compared with the parental strain FP2. Although HM053-36 and FP2-7 showed GUS activity located mainly at lateral root emergence points, HM053-36 consistently showed stronger signals and expressed the nifH gene at a level 278 fold higher than strain FP2 in planta, according to qPCR data. Conclusions: HM053, a spontaneous mutant in GS, increased wheat root and shoot dry weight when compared to the wild-type FP2. HM053 ability to excrete ammonium and fix nitrogen constitutively, even in the presence of high NH 4 + concentration, could explain why this mutant has a higher potential to promote plant growth than FP2 and suggests HM053 as a potential nitrogen biofertilizer. However, HM053 should be tested under field conditions to evaluate its abilities to compete with indigenous microflora.","bibtype":"article","author":"Santos, K. F.D.N. and Moure, V. R. and Hauer, V. and Santos, A. R.S. and Donatti, L. and Galvão, C. W. and Pedrosa, F. O. and Souza, E. M. and Wassem, R. and Steffens, M. B.R.","journal":"Plant and Soil","number":"1-2","bibtex":"@article{\n title = {Wheat colonization by an Azospirillum brasilense ammonium-excreting strain reveals upregulation of nitrogenase and superior plant growth promotion},\n type = {article},\n year = {2017},\n identifiers = {[object Object]},\n keywords = {Ammonium-excreting mutant,Azospirillum brasilense,Biofertilizer,Glutamine synthetase,glnA gene},\n pages = {245-255},\n volume = {415},\n websites = {http://link.springer.com/10.1007/s11104-016-3140-6},\n month = {6},\n id = {dc7fc732-0913-36b9-b741-f17fbc42813f},\n created = {2019-02-04T15:29:24.682Z},\n file_attached = {false},\n profile_id = {81a97cb0-31b8-3385-bd0e-efcfffedaada},\n group_id = {b954b4a7-36ce-39f9-9501-2e3795a979e9},\n last_modified = {2019-02-04T15:29:24.682Z},\n read = {false},\n starred = {false},\n authored = {false},\n confirmed = {true},\n hidden = {false},\n citation_key = {Santos2017a},\n source_type = {article},\n language = {en},\n notes = {CAPES qualis 2013-2016 CB I: B1},\n private_publication = {false},\n abstract = {© 2016, Springer International Publishing Switzerland. Aims: In this work, an ammonium-excreting strain (HM053) of A. brasilense was further characterized genetically and biochemically, and its abilities to colonize and promote wheat growth were determined. Methods: Immunoblot, reverse transcription-qPCR, and DNA sequencing were used for HM053 characterization. To analyze wheat-A. brasilense interaction nifH::gusA fusions in the wild-type FP2 (FP2-7) and HM053 (HM053-36) backgrounds were employed. Results: HM053 glutamine synthetase (GS) was not adenylylated in response to an ammonium shock or under any condition tested. Sequencing of the glnA gene revealed a substitution of a proline residue by a leucine at position 347 of the GS. Under axenic growth condition, HM053 was capable of colonizing the surface of wheat roots and increased by 30 and 49% the shoot and root dry weight, respectively, when compared with uninoculated plants, and by 30 and 31% when compared with the parental strain FP2. Although HM053-36 and FP2-7 showed GUS activity located mainly at lateral root emergence points, HM053-36 consistently showed stronger signals and expressed the nifH gene at a level 278 fold higher than strain FP2 in planta, according to qPCR data. Conclusions: HM053, a spontaneous mutant in GS, increased wheat root and shoot dry weight when compared to the wild-type FP2. HM053 ability to excrete ammonium and fix nitrogen constitutively, even in the presence of high NH 4 + concentration, could explain why this mutant has a higher potential to promote plant growth than FP2 and suggests HM053 as a potential nitrogen biofertilizer. However, HM053 should be tested under field conditions to evaluate its abilities to compete with indigenous microflora.},\n bibtype = {article},\n author = {Santos, K. F.D.N. and Moure, V. R. and Hauer, V. and Santos, A. R.S. and Donatti, L. and Galvão, C. W. and Pedrosa, F. O. and Souza, E. M. and Wassem, R. and Steffens, M. 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