The sigma factor sigma s affects antibiotic production and biological control activity of Pseudomonas fluorescens Pf-5. Sarniguet, A., Kraus, J., Henkels, M. D., Muehlchen, A. M., & Loper, J. E. Proc Natl Acad Sci U S A, 92(26):12255–12259, 12, 1995. [PubMed Central:\hrefhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC40335PMC40335] [DOI:\hrefhttps://dx.doi.org/10.1073/pnas.92.26.1225510.1073/pnas.92.26.12255] [PubMed:\hrefhttps://www.ncbi.nlm.nih.gov/pubmed/120492120492] abstract bibtex Pseudomonas fluorescens Pf-5, a rhizosphere-inhabiting bacterium that suppresses several soilborne pathogens of plants, produces the antibiotics pyrrolnitrin, pyoluteorin, and 2,4-diacetylphloroglucinol. A gene necessary for pyrrolnitrin production by Pf-5 was identified as rpoS, which encodes the stationary-phase sigma factor sigma s. Several pleiotropic effects of an rpoS mutation in Escherichia coli also were observed in an RpoS- mutant of Pf-5. These included sensitivities of stationary-phase cells to stresses imposed by hydrogen peroxide or high salt concentration. A plasmid containing the cloned wild-type rpoS gene restored pyrrolnitrin production and stress tolerance to the RpoS- mutant of Pf-5. The RpoS- mutant overproduced pyoluteorin and 2,4-diacetyl-phloroglucinol, two antibiotics that inhibit growth of the phytopathogenic fungus Pythium ultimum, and was superior to the wild type in suppression of seedling damping-off of cucumber caused by Pythium ultimum. When inoculated onto cucumber seed at high cell densities, the RpoS- mutant did not survive as well as the wild-type strain on surfaces of developing seedlings. Other stationary-phase-specific phenotypes of Pf-5, such as the production of cyanide and extracellular protease(s) were expressed by the RpoS- mutant, suggesting that sigma s is only one of the sigma factors required for the transcription of genes in stationary-phase cells of P. fluorescens. These results indicate that a sigma factor encoded by rpoS influences antibiotic production, biological control activity, and survival of P. fluorescens on plant surfaces.
@Article{sarniguet1995,
Author="Sarniguet, A. and Kraus, J. and Henkels, M. D. and Muehlchen, A. M. and Loper, J. E. ",
Title="{{T}he sigma factor sigma s affects antibiotic production and biological control activity of {P}seudomonas fluorescens {P}f-5}",
Journal="Proc Natl Acad Sci U S A",
Year="1995",
Volume="92",
Number="26",
Pages="12255--12259",
Month="12",
Abstract={Pseudomonas fluorescens Pf-5, a rhizosphere-inhabiting bacterium that suppresses several soilborne pathogens of plants, produces the antibiotics pyrrolnitrin, pyoluteorin, and 2,4-diacetylphloroglucinol. A gene necessary for pyrrolnitrin production by Pf-5 was identified as rpoS, which encodes the stationary-phase sigma factor sigma s. Several pleiotropic effects of an rpoS mutation in Escherichia coli also were observed in an RpoS- mutant of Pf-5. These included sensitivities of stationary-phase cells to stresses imposed by hydrogen peroxide or high salt concentration. A plasmid containing the cloned wild-type rpoS gene restored pyrrolnitrin production and stress tolerance to the RpoS- mutant of Pf-5. The RpoS- mutant overproduced pyoluteorin and 2,4-diacetyl-phloroglucinol, two antibiotics that inhibit growth of the phytopathogenic fungus Pythium ultimum, and was superior to the wild type in suppression of seedling damping-off of cucumber caused by Pythium ultimum. When inoculated onto cucumber seed at high cell densities, the RpoS- mutant did not survive as well as the wild-type strain on surfaces of developing seedlings. Other stationary-phase-specific phenotypes of Pf-5, such as the production of cyanide and extracellular protease(s) were expressed by the RpoS- mutant, suggesting that sigma s is only one of the sigma factors required for the transcription of genes in stationary-phase cells of P. fluorescens. These results indicate that a sigma factor encoded by rpoS influences antibiotic production, biological control activity, and survival of P. fluorescens on plant surfaces.},
Note={[PubMed Central:\href{https://www.ncbi.nlm.nih.gov/pmc/articles/PMC40335}{PMC40335}] [DOI:\href{https://dx.doi.org/10.1073/pnas.92.26.12255}{10.1073/pnas.92.26.12255}] [PubMed:\href{https://www.ncbi.nlm.nih.gov/pubmed/120492}{120492}] }
}
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{"_id":"YYnnoQgNbpc4Nmz4e","bibbaseid":"sarniguet-kraus-henkels-muehlchen-loper-thesigmafactorsigmasaffectsantibioticproductionandbiologicalcontrolactivityofpseudomonasfluorescenspf5-1995","author_short":["Sarniguet, A.","Kraus, J.","Henkels, M. D.","Muehlchen, A. M.","Loper, J. E."],"bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Sarniguet"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Kraus"],"firstnames":["J."],"suffixes":[]},{"propositions":[],"lastnames":["Henkels"],"firstnames":["M.","D."],"suffixes":[]},{"propositions":[],"lastnames":["Muehlchen"],"firstnames":["A.","M."],"suffixes":[]},{"propositions":[],"lastnames":["Loper"],"firstnames":["J.","E."],"suffixes":[]}],"title":"The sigma factor sigma s affects antibiotic production and biological control activity of Pseudomonas fluorescens Pf-5","journal":"Proc Natl Acad Sci U S A","year":"1995","volume":"92","number":"26","pages":"12255–12259","month":"12","abstract":"Pseudomonas fluorescens Pf-5, a rhizosphere-inhabiting bacterium that suppresses several soilborne pathogens of plants, produces the antibiotics pyrrolnitrin, pyoluteorin, and 2,4-diacetylphloroglucinol. A gene necessary for pyrrolnitrin production by Pf-5 was identified as rpoS, which encodes the stationary-phase sigma factor sigma s. Several pleiotropic effects of an rpoS mutation in Escherichia coli also were observed in an RpoS- mutant of Pf-5. These included sensitivities of stationary-phase cells to stresses imposed by hydrogen peroxide or high salt concentration. A plasmid containing the cloned wild-type rpoS gene restored pyrrolnitrin production and stress tolerance to the RpoS- mutant of Pf-5. The RpoS- mutant overproduced pyoluteorin and 2,4-diacetyl-phloroglucinol, two antibiotics that inhibit growth of the phytopathogenic fungus Pythium ultimum, and was superior to the wild type in suppression of seedling damping-off of cucumber caused by Pythium ultimum. When inoculated onto cucumber seed at high cell densities, the RpoS- mutant did not survive as well as the wild-type strain on surfaces of developing seedlings. Other stationary-phase-specific phenotypes of Pf-5, such as the production of cyanide and extracellular protease(s) were expressed by the RpoS- mutant, suggesting that sigma s is only one of the sigma factors required for the transcription of genes in stationary-phase cells of P. fluorescens. These results indicate that a sigma factor encoded by rpoS influences antibiotic production, biological control activity, and survival of P. fluorescens on plant surfaces.","note":"[PubMed Central:\\hrefhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC40335PMC40335] [DOI:\\hrefhttps://dx.doi.org/10.1073/pnas.92.26.1225510.1073/pnas.92.26.12255] [PubMed:\\hrefhttps://www.ncbi.nlm.nih.gov/pubmed/120492120492] ","bibtex":"@Article{sarniguet1995,\n Author=\"Sarniguet, A. and Kraus, J. and Henkels, M. D. and Muehlchen, A. M. and Loper, J. E. \",\n Title=\"{{T}he sigma factor sigma s affects antibiotic production and biological control activity of {P}seudomonas fluorescens {P}f-5}\",\n Journal=\"Proc Natl Acad Sci U S A\",\n Year=\"1995\",\n Volume=\"92\",\n Number=\"26\",\n Pages=\"12255--12259\",\n Month=\"12\",\n Abstract={Pseudomonas fluorescens Pf-5, a rhizosphere-inhabiting bacterium that suppresses several soilborne pathogens of plants, produces the antibiotics pyrrolnitrin, pyoluteorin, and 2,4-diacetylphloroglucinol. A gene necessary for pyrrolnitrin production by Pf-5 was identified as rpoS, which encodes the stationary-phase sigma factor sigma s. Several pleiotropic effects of an rpoS mutation in Escherichia coli also were observed in an RpoS- mutant of Pf-5. These included sensitivities of stationary-phase cells to stresses imposed by hydrogen peroxide or high salt concentration. A plasmid containing the cloned wild-type rpoS gene restored pyrrolnitrin production and stress tolerance to the RpoS- mutant of Pf-5. The RpoS- mutant overproduced pyoluteorin and 2,4-diacetyl-phloroglucinol, two antibiotics that inhibit growth of the phytopathogenic fungus Pythium ultimum, and was superior to the wild type in suppression of seedling damping-off of cucumber caused by Pythium ultimum. When inoculated onto cucumber seed at high cell densities, the RpoS- mutant did not survive as well as the wild-type strain on surfaces of developing seedlings. Other stationary-phase-specific phenotypes of Pf-5, such as the production of cyanide and extracellular protease(s) were expressed by the RpoS- mutant, suggesting that sigma s is only one of the sigma factors required for the transcription of genes in stationary-phase cells of P. fluorescens. These results indicate that a sigma factor encoded by rpoS influences antibiotic production, biological control activity, and survival of P. fluorescens on plant surfaces.},\n Note={[PubMed Central:\\href{https://www.ncbi.nlm.nih.gov/pmc/articles/PMC40335}{PMC40335}] [DOI:\\href{https://dx.doi.org/10.1073/pnas.92.26.12255}{10.1073/pnas.92.26.12255}] [PubMed:\\href{https://www.ncbi.nlm.nih.gov/pubmed/120492}{120492}] }\n}\n\n\n","author_short":["Sarniguet, A.","Kraus, J.","Henkels, M. D.","Muehlchen, A. M.","Loper, J. E."],"key":"sarniguet1995","id":"sarniguet1995","bibbaseid":"sarniguet-kraus-henkels-muehlchen-loper-thesigmafactorsigmasaffectsantibioticproductionandbiologicalcontrolactivityofpseudomonasfluorescenspf5-1995","role":"author","urls":{},"metadata":{"authorlinks":{}},"html":""},"bibtype":"article","biburl":"https://bibbase.org/network/files/7omCQCMxvjPCQZfjb","dataSources":["4EGaEr5EKz54T8jGa"],"keywords":[],"search_terms":["sigma","factor","sigma","affects","antibiotic","production","biological","control","activity","pseudomonas","fluorescens","sarniguet","kraus","henkels","muehlchen","loper"],"title":"The sigma factor sigma s affects antibiotic production and biological control activity of Pseudomonas fluorescens Pf-5","year":1995}