Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Sato, T., Vries, R. G., Snippert, H. J., van de Wetering, M., Barker, N., Stange, D. E., van Es, J. H., Abo, A., Kujala, P., Peters, P. J., & Clevers, H. Nature, 459(7244):262–265, May, 2009. Publisher: Nature Publishing Group![Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Sato et al. present a method for establishing long-term culture conditions whereby single stem cells or single crypt structures isolated from intestinal crypts can generate organoids with all the differentiated cell types and architecture of intestinal crypts present in adult mammals. The stem cells they used to start the cultures were marked by Lrg5+, a protein that in an earlier study had been shown to mark six cycling stem cells that renew the rapidly self-renewing tissue of the intestine. They conclude that intestinal crypt-villus units are self-organizing structures, which can be built from a single stem cell in the absence of a cellular niche.
@article{sato_single_2009,
title = {Single {Lgr5} stem cells build crypt-villus structures in vitro without a mesenchymal niche},
volume = {459},
copyright = {2009 Macmillan Publishers Limited. All rights reserved},
issn = {1476-4687},
url = {https://www.nature.com/articles/nature07935},
doi = {10.1038/nature07935},
abstract = {Sato et al. present a method for establishing long-term culture conditions whereby single stem cells or single crypt structures isolated from intestinal crypts can generate organoids with all the differentiated cell types and architecture of intestinal crypts present in adult mammals. The stem cells they used to start the cultures were marked by Lrg5+, a protein that in an earlier study had been shown to mark six cycling stem cells that renew the rapidly self-renewing tissue of the intestine. They conclude that intestinal crypt-villus units are self-organizing structures, which can be built from a single stem cell in the absence of a cellular niche.},
language = {en},
number = {7244},
urldate = {2024-11-25},
journal = {Nature},
author = {Sato, Toshiro and Vries, Robert G. and Snippert, Hugo J. and van de Wetering, Marc and Barker, Nick and Stange, Daniel E. and van Es, Johan H. and Abo, Arie and Kujala, Pekka and Peters, Peter J. and Clevers, Hans},
month = may,
year = {2009},
note = {Publisher: Nature Publishing Group},
keywords = {Humanities and Social Sciences, Science, multidisciplinary},
pages = {262--265},
}
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