@article{Sawhney2022a,
abstract = {Host inflammatory responses are key to protection against injury; however, persistent inflammation is detrimental and contributes to morbidity and mortality. Herein, we demonstrated the anti-inflammatory role of Arteannuin-B (1) and its new spirocyclic-2-isoxazoline derivative JR-9 and their side effects in acute inflammatory condition in vivo using LPS-induced cytokines assay, carrageenan-induced paw edema, acetic acid-induced writhing and tail immersion. The results show that the spirocyclic-2-isoxazoline derivative is a potent anti-inflammatory agent with minimal cell toxicity as compared to Arteannuin-B. In addition, the efficacies of these compounds were also validated by flow cytometric, computational, and histopathological analysis. Our results show that the anti-inflammatory response of JR-9 significantly reduces the ability of mouse macrophages to produce NO, TNF-{\&}alpha;, and IL-6 following LPS stimulation. Therefore, JR-9 is a prospective candidate for the development of anti-inflammatory drugs and its molecular mechanism is likely related to the regulation of NF-{\&}kappa;B and MAPK signaling pathway.},
author = {Sawhney, Gifty and Rasool, Javeed Ur and Saroch, Diksha and Ozturk, Mumin and Brombacher, Frank and Ahmad, Bilal and Bhagat, Asha and Ali, Asif and Parihar, Suraj P. and Ahmed, Zabeer},
doi = {10.3390/MOLECULES27228068},
file = {:C$\backslash$:/Users/01462563/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Sawhney et al. - 2022 - Arteannuin-B and (3-chlorophenyl)-2-spiroisoxazoline derivative exhibit anti-inflammatory effects in LPS-activat.pdf:pdf},
issn = {1420-3049},
journal = {Molecules},
keywords = {2,6,Arteannuin,B,BALB/c mice,IL,LPS,NF,OA,RAW 264.7 cells,TNF,cytokines,fund{\_}ack,iNOS,inflammation,isoxazoline derivative,original,spirocyclic,$\alpha$,$\kappa$B},
mendeley-tags = {OA,fund{\_}ack,original},
month = {nov},
pages = {8068},
pmid = {36432169},
publisher = {Multidisciplinary Digital Publishing Institute},
title = {{Arteannuin-B and (3-chlorophenyl)-2-spiroisoxazoline derivative exhibit anti-inflammatory effects in LPS-activated RAW 264.7 macrophages and BALB/c mice-induced proinflammatory responses via downregulation of NF-kappaB/P38 MAPK signaling}},
url = {https://www.mdpi.com/1420-3049/27/22/8068/htm https://www.mdpi.com/1420-3049/27/22/8068},
volume = {27},
year = {2022}
}

{"_id":"ZDEHqdupfoEgDxBLm","bibbaseid":"sawhney-rasool-saroch-ozturk-brombacher-ahmad-bhagat-ali-etal-arteannuinband3chlorophenyl2spiroisoxazolinederivativeexhibitantiinflammatoryeffectsinlpsactivatedraw2647macrophagesandbalbcmiceinducedproinflammatoryresponsesviadownregulationofnfkappabp38mapksignaling-2022","author_short":["Sawhney, G.","Rasool, J. U.","Saroch, D.","Ozturk, M.","Brombacher, F.","Ahmad, B.","Bhagat, A.","Ali, A.","Parihar, S. P.","Ahmed, Z."],"bibdata":{"bibtype":"article","type":"article","abstract":"Host inflammatory responses are key to protection against injury; however, persistent inflammation is detrimental and contributes to morbidity and mortality. Herein, we demonstrated the anti-inflammatory role of Arteannuin-B (1) and its new spirocyclic-2-isoxazoline derivative JR-9 and their side effects in acute inflammatory condition in vivo using LPS-induced cytokines assay, carrageenan-induced paw edema, acetic acid-induced writhing and tail immersion. The results show that the spirocyclic-2-isoxazoline derivative is a potent anti-inflammatory agent with minimal cell toxicity as compared to Arteannuin-B. In addition, the efficacies of these compounds were also validated by flow cytometric, computational, and histopathological analysis. Our results show that the anti-inflammatory response of JR-9 significantly reduces the ability of mouse macrophages to produce NO, TNF-α, and IL-6 following LPS stimulation. Therefore, JR-9 is a prospective candidate for the development of anti-inflammatory drugs and its molecular mechanism is likely related to the regulation of NF-κB and MAPK signaling pathway.","author":[{"propositions":[],"lastnames":["Sawhney"],"firstnames":["Gifty"],"suffixes":[]},{"propositions":[],"lastnames":["Rasool"],"firstnames":["Javeed","Ur"],"suffixes":[]},{"propositions":[],"lastnames":["Saroch"],"firstnames":["Diksha"],"suffixes":[]},{"propositions":[],"lastnames":["Ozturk"],"firstnames":["Mumin"],"suffixes":[]},{"propositions":[],"lastnames":["Brombacher"],"firstnames":["Frank"],"suffixes":[]},{"propositions":[],"lastnames":["Ahmad"],"firstnames":["Bilal"],"suffixes":[]},{"propositions":[],"lastnames":["Bhagat"],"firstnames":["Asha"],"suffixes":[]},{"propositions":[],"lastnames":["Ali"],"firstnames":["Asif"],"suffixes":[]},{"propositions":[],"lastnames":["Parihar"],"firstnames":["Suraj","P."],"suffixes":[]},{"propositions":[],"lastnames":["Ahmed"],"firstnames":["Zabeer"],"suffixes":[]}],"doi":"10.3390/MOLECULES27228068","file":":C$\\$:/Users/01462563/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Sawhney et al. - 2022 - Arteannuin-B and (3-chlorophenyl)-2-spiroisoxazoline derivative exhibit anti-inflammatory effects in LPS-activat.pdf:pdf","issn":"1420-3049","journal":"Molecules","keywords":"2,6,Arteannuin,B,BALB/c mice,IL,LPS,NF,OA,RAW 264.7 cells,TNF,cytokines,fund_ack,iNOS,inflammation,isoxazoline derivative,original,spirocyclic,$α$,$κ$B","mendeley-tags":"OA,fund_ack,original","month":"nov","pages":"8068","pmid":"36432169","publisher":"Multidisciplinary Digital Publishing Institute","title":"Arteannuin-B and (3-chlorophenyl)-2-spiroisoxazoline derivative exhibit anti-inflammatory effects in LPS-activated RAW 264.7 macrophages and BALB/c mice-induced proinflammatory responses via downregulation of NF-kappaB/P38 MAPK signaling","url":"https://www.mdpi.com/1420-3049/27/22/8068/htm https://www.mdpi.com/1420-3049/27/22/8068","volume":"27","year":"2022","bibtex":"@article{Sawhney2022a,\r\nabstract = {Host inflammatory responses are key to protection against injury; however, persistent inflammation is detrimental and contributes to morbidity and mortality. Herein, we demonstrated the anti-inflammatory role of Arteannuin-B (1) and its new spirocyclic-2-isoxazoline derivative JR-9 and their side effects in acute inflammatory condition in vivo using LPS-induced cytokines assay, carrageenan-induced paw edema, acetic acid-induced writhing and tail immersion. The results show that the spirocyclic-2-isoxazoline derivative is a potent anti-inflammatory agent with minimal cell toxicity as compared to Arteannuin-B. In addition, the efficacies of these compounds were also validated by flow cytometric, computational, and histopathological analysis. Our results show that the anti-inflammatory response of JR-9 significantly reduces the ability of mouse macrophages to produce NO, TNF-{\\&}alpha;, and IL-6 following LPS stimulation. Therefore, JR-9 is a prospective candidate for the development of anti-inflammatory drugs and its molecular mechanism is likely related to the regulation of NF-{\\&}kappa;B and MAPK signaling pathway.},\r\nauthor = {Sawhney, Gifty and Rasool, Javeed Ur and Saroch, Diksha and Ozturk, Mumin and Brombacher, Frank and Ahmad, Bilal and Bhagat, Asha and Ali, Asif and Parihar, Suraj P. and Ahmed, Zabeer},\r\ndoi = {10.3390/MOLECULES27228068},\r\nfile = {:C$\\backslash$:/Users/01462563/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Sawhney et al. - 2022 - Arteannuin-B and (3-chlorophenyl)-2-spiroisoxazoline derivative exhibit anti-inflammatory effects in LPS-activat.pdf:pdf},\r\nissn = {1420-3049},\r\njournal = {Molecules},\r\nkeywords = {2,6,Arteannuin,B,BALB/c mice,IL,LPS,NF,OA,RAW 264.7 cells,TNF,cytokines,fund{\\_}ack,iNOS,inflammation,isoxazoline derivative,original,spirocyclic,$\\alpha$,$\\kappa$B},\r\nmendeley-tags = {OA,fund{\\_}ack,original},\r\nmonth = {nov},\r\npages = {8068},\r\npmid = {36432169},\r\npublisher = {Multidisciplinary Digital Publishing Institute},\r\ntitle = {{Arteannuin-B and (3-chlorophenyl)-2-spiroisoxazoline derivative exhibit anti-inflammatory effects in LPS-activated RAW 264.7 macrophages and BALB/c mice-induced proinflammatory responses via downregulation of NF-kappaB/P38 MAPK signaling}},\r\nurl = {https://www.mdpi.com/1420-3049/27/22/8068/htm https://www.mdpi.com/1420-3049/27/22/8068},\r\nvolume = {27},\r\nyear = {2022}\r\n}\r\n","author_short":["Sawhney, G.","Rasool, J. U.","Saroch, D.","Ozturk, M.","Brombacher, F.","Ahmad, B.","Bhagat, A.","Ali, A.","Parihar, S. P.","Ahmed, Z."],"key":"Sawhney2022a","id":"Sawhney2022a","bibbaseid":"sawhney-rasool-saroch-ozturk-brombacher-ahmad-bhagat-ali-etal-arteannuinband3chlorophenyl2spiroisoxazolinederivativeexhibitantiinflammatoryeffectsinlpsactivatedraw2647macrophagesandbalbcmiceinducedproinflammatoryresponsesviadownregulationofnfkappabp38mapksignaling-2022","role":"author","urls":{"Paper":"https://www.mdpi.com/1420-3049/27/22/8068/htm https://www.mdpi.com/1420-3049/27/22/8068"},"keyword":["2","6","Arteannuin","B","BALB/c mice","IL","LPS","NF","OA","RAW 264.7 cells","TNF","cytokines","fund_ack","iNOS","inflammation","isoxazoline derivative","original","spirocyclic","$α$","$κ$B"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://drive.google.com/uc?export=download&id=1-JLqZ7RwZ3VC2d6ErLGHAtOeMRS_7GCz","dataSources":["Krmt6gt9ktB2s6ARh"],"keywords":["2","6","arteannuin","b","balb/c mice","il","lps","nf","oa","raw 264.7 cells","tnf","cytokines","fund_ack","inos","inflammation","isoxazoline derivative","original","spirocyclic","$α$","$κ$b"],"search_terms":["arteannuin","chlorophenyl","spiroisoxazoline","derivative","exhibit","anti","inflammatory","effects","lps","activated","raw","264","macrophages","balb","mice","induced","proinflammatory","responses","via","downregulation","kappab","p38","mapk","signaling","sawhney","rasool","saroch","ozturk","brombacher","ahmad","bhagat","ali","parihar","ahmed"],"title":"Arteannuin-B and (3-chlorophenyl)-2-spiroisoxazoline derivative exhibit anti-inflammatory effects in LPS-activated RAW 264.7 macrophages and BALB/c mice-induced proinflammatory responses via downregulation of NF-kappaB/P38 MAPK signaling","year":2022}