Generation and modulation of hepatocellular carcinoma circulating cells: a new experimental model. Scatton, O., Chiappini, F., Liu, X., Riou, P., Marconi, A., Debuire, B., Azoulay, D., & Lemoine, A. J Surg Res, 150(2):183–9, 2008.
Generation and modulation of hepatocellular carcinoma circulating cells: a new experimental model [link]Paper  doi  abstract   bibtex   
BACKGROUND: To establish a new experimental model of human hepatocellular carcinoma by orthotopic implantation of tumoral cells with its subsequent removal, to generate and modulate circulating tumoral cells. MATERIALS AND METHODS: Three human hepatoma cell lines (HepG2, PLC/PRF, and Mahlavu) were orthotopically implanted under the Glisson's capsule of the left lateral lobe of the liver in a total of 56 non-obese diabetic/severe combined immunodeficiency mice. Tumor removal was performed 30 d after injection, and a laparotomy without tumor removal was done in control mice. Generation of circulating cells was monitored by flow cytometry using fluorescein isothiocyanate-conjugated anti-HLA antibody. RESULTS: In 26 mice implanted with Mahlavu cells, 20 developed a unique tumor allowing a resection (77%), which was technically feasible in 80% of cases. The overall perioperative mortality was 30% (3/10) after resection; no mortality was observed in the control group. The circulating tumoral cells decreased dramatically after resection of the tumor as compared with control mice. CONCLUSION: This new model is feasible and may be an interesting useful tool to study the hepatocellular carcinoma metastatic process and is consistent with the human clinical practice.
@article{scatton_generation_2008,
	title = {Generation and modulation of hepatocellular carcinoma circulating cells: a new experimental model},
	volume = {150},
	issn = {1095-8673},
	url = {http://dx.doi.org/10.1016/j.jss.2008.03.052},
	doi = {10.1016/j.jss.2008.03.052},
	abstract = {BACKGROUND: To establish a new experimental model of human hepatocellular carcinoma by orthotopic implantation of tumoral cells with its subsequent removal, to generate and modulate circulating tumoral cells. MATERIALS AND METHODS: Three human hepatoma cell lines (HepG2, PLC/PRF, and Mahlavu) were orthotopically implanted under the Glisson's capsule of the left lateral lobe of the liver in a total of 56 non-obese diabetic/severe combined immunodeficiency mice. Tumor removal was performed 30 d after injection, and a laparotomy without tumor removal was done in control mice. Generation of circulating cells was monitored by flow cytometry using fluorescein isothiocyanate-conjugated anti-HLA antibody. RESULTS: In 26 mice implanted with Mahlavu cells, 20 developed a unique tumor allowing a resection (77\%), which was technically feasible in 80\% of cases. The overall perioperative mortality was 30\% (3/10) after resection; no mortality was observed in the control group. The circulating tumoral cells decreased dramatically after resection of the tumor as compared with control mice. CONCLUSION: This new model is feasible and may be an interesting useful tool to study the hepatocellular carcinoma metastatic process and is consistent with the human clinical practice.},
	number = {2},
	journal = {J Surg Res},
	author = {Scatton, Olivier and Chiappini, Franck and Liu, Xu-Hui and Riou, Philippe and Marconi, Anthony and Debuire, Brigitte and Azoulay, Daniel and Lemoine, Antoinette},
	year = {2008},
	pages = {183--9}
}

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