@article{scatton_generation_2008,
	title = {Generation and modulation of hepatocellular carcinoma circulating cells: a new experimental model},
	volume = {150},
	issn = {1095-8673},
	url = {http://dx.doi.org/10.1016/j.jss.2008.03.052},
	doi = {10.1016/j.jss.2008.03.052},
	abstract = {BACKGROUND: To establish a new experimental model of human hepatocellular carcinoma by orthotopic implantation of tumoral cells with its subsequent removal, to generate and modulate circulating tumoral cells. MATERIALS AND METHODS: Three human hepatoma cell lines (HepG2, PLC/PRF, and Mahlavu) were orthotopically implanted under the Glisson's capsule of the left lateral lobe of the liver in a total of 56 non-obese diabetic/severe combined immunodeficiency mice. Tumor removal was performed 30 d after injection, and a laparotomy without tumor removal was done in control mice. Generation of circulating cells was monitored by flow cytometry using fluorescein isothiocyanate-conjugated anti-HLA antibody. RESULTS: In 26 mice implanted with Mahlavu cells, 20 developed a unique tumor allowing a resection (77\%), which was technically feasible in 80\% of cases. The overall perioperative mortality was 30\% (3/10) after resection; no mortality was observed in the control group. The circulating tumoral cells decreased dramatically after resection of the tumor as compared with control mice. CONCLUSION: This new model is feasible and may be an interesting useful tool to study the hepatocellular carcinoma metastatic process and is consistent with the human clinical practice.},
	number = {2},
	journal = {J Surg Res},
	author = {Scatton, Olivier and Chiappini, Franck and Liu, Xu-Hui and Riou, Philippe and Marconi, Anthony and Debuire, Brigitte and Azoulay, Daniel and Lemoine, Antoinette},
	year = {2008},
	pages = {183--9},
}

{"_id":"B2cJfye3muhs9AB5H","bibbaseid":"scatton-chiappini-liu-riou-marconi-debuire-azoulay-lemoine-generationandmodulationofhepatocellularcarcinomacirculatingcellsanewexperimentalmodel-2008","author_short":["Scatton, O.","Chiappini, F.","Liu, X.","Riou, P.","Marconi, A.","Debuire, B.","Azoulay, D.","Lemoine, A."],"bibdata":{"bibtype":"article","type":"article","title":"Generation and modulation of hepatocellular carcinoma circulating cells: a new experimental model","volume":"150","issn":"1095-8673","url":"http://dx.doi.org/10.1016/j.jss.2008.03.052","doi":"10.1016/j.jss.2008.03.052","abstract":"BACKGROUND: To establish a new experimental model of human hepatocellular carcinoma by orthotopic implantation of tumoral cells with its subsequent removal, to generate and modulate circulating tumoral cells. MATERIALS AND METHODS: Three human hepatoma cell lines (HepG2, PLC/PRF, and Mahlavu) were orthotopically implanted under the Glisson's capsule of the left lateral lobe of the liver in a total of 56 non-obese diabetic/severe combined immunodeficiency mice. Tumor removal was performed 30 d after injection, and a laparotomy without tumor removal was done in control mice. Generation of circulating cells was monitored by flow cytometry using fluorescein isothiocyanate-conjugated anti-HLA antibody. RESULTS: In 26 mice implanted with Mahlavu cells, 20 developed a unique tumor allowing a resection (77%), which was technically feasible in 80% of cases. The overall perioperative mortality was 30% (3/10) after resection; no mortality was observed in the control group. The circulating tumoral cells decreased dramatically after resection of the tumor as compared with control mice. CONCLUSION: This new model is feasible and may be an interesting useful tool to study the hepatocellular carcinoma metastatic process and is consistent with the human clinical practice.","number":"2","journal":"J Surg Res","author":[{"propositions":[],"lastnames":["Scatton"],"firstnames":["Olivier"],"suffixes":[]},{"propositions":[],"lastnames":["Chiappini"],"firstnames":["Franck"],"suffixes":[]},{"propositions":[],"lastnames":["Liu"],"firstnames":["Xu-Hui"],"suffixes":[]},{"propositions":[],"lastnames":["Riou"],"firstnames":["Philippe"],"suffixes":[]},{"propositions":[],"lastnames":["Marconi"],"firstnames":["Anthony"],"suffixes":[]},{"propositions":[],"lastnames":["Debuire"],"firstnames":["Brigitte"],"suffixes":[]},{"propositions":[],"lastnames":["Azoulay"],"firstnames":["Daniel"],"suffixes":[]},{"propositions":[],"lastnames":["Lemoine"],"firstnames":["Antoinette"],"suffixes":[]}],"year":"2008","pages":"183–9","bibtex":"@article{scatton_generation_2008,\n\ttitle = {Generation and modulation of hepatocellular carcinoma circulating cells: a new experimental model},\n\tvolume = {150},\n\tissn = {1095-8673},\n\turl = {http://dx.doi.org/10.1016/j.jss.2008.03.052},\n\tdoi = {10.1016/j.jss.2008.03.052},\n\tabstract = {BACKGROUND: To establish a new experimental model of human hepatocellular carcinoma by orthotopic implantation of tumoral cells with its subsequent removal, to generate and modulate circulating tumoral cells. MATERIALS AND METHODS: Three human hepatoma cell lines (HepG2, PLC/PRF, and Mahlavu) were orthotopically implanted under the Glisson's capsule of the left lateral lobe of the liver in a total of 56 non-obese diabetic/severe combined immunodeficiency mice. Tumor removal was performed 30 d after injection, and a laparotomy without tumor removal was done in control mice. Generation of circulating cells was monitored by flow cytometry using fluorescein isothiocyanate-conjugated anti-HLA antibody. RESULTS: In 26 mice implanted with Mahlavu cells, 20 developed a unique tumor allowing a resection (77\\%), which was technically feasible in 80\\% of cases. The overall perioperative mortality was 30\\% (3/10) after resection; no mortality was observed in the control group. The circulating tumoral cells decreased dramatically after resection of the tumor as compared with control mice. CONCLUSION: This new model is feasible and may be an interesting useful tool to study the hepatocellular carcinoma metastatic process and is consistent with the human clinical practice.},\n\tnumber = {2},\n\tjournal = {J Surg Res},\n\tauthor = {Scatton, Olivier and Chiappini, Franck and Liu, Xu-Hui and Riou, Philippe and Marconi, Anthony and Debuire, Brigitte and Azoulay, Daniel and Lemoine, Antoinette},\n\tyear = {2008},\n\tpages = {183--9},\n}\n\n","author_short":["Scatton, O.","Chiappini, F.","Liu, X.","Riou, P.","Marconi, A.","Debuire, B.","Azoulay, D.","Lemoine, A."],"key":"scatton_generation_2008","id":"scatton_generation_2008","bibbaseid":"scatton-chiappini-liu-riou-marconi-debuire-azoulay-lemoine-generationandmodulationofhepatocellularcarcinomacirculatingcellsanewexperimentalmodel-2008","role":"author","urls":{"Paper":"http://dx.doi.org/10.1016/j.jss.2008.03.052"},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"http://bibbase.org/zotero/biblioial","dataSources":["ftoP3zPyb2N3b9Noc"],"keywords":[],"search_terms":["generation","modulation","hepatocellular","carcinoma","circulating","cells","new","experimental","model","scatton","chiappini","liu","riou","marconi","debuire","azoulay","lemoine"],"title":"Generation and modulation of hepatocellular carcinoma circulating cells: a new experimental model","year":2008}