Actin chromobody imaging reveals sub-organellar actin dynamics. Schiavon, C. R, Zhang, T., Zhao, B., Moore, A. S, Wales, P., Andrade, L. R, Wu, M., Sung, T., Dayn, Y., Feng, J. W, Quintero, O. A, Shadel, G. S, Grosse, R., & Manor, U. Nature Methods, 17(9):917–921, 2020.
Actin chromobody imaging reveals sub-organellar actin dynamics [link]Paper  doi  abstract   bibtex   
The actin cytoskeleton plays multiple critical roles in cells, from cell migration to organelle dynamics. The small and transient actin structures regulating organelle dynamics are challenging to detect with fluorescence microscopy, making it difficult to determine whether actin filaments are directly associated with specific membranes. To address these limitations, we developed fluorescent-protein-tagged actin nanobodies, termed ‘actin chromobodies' (ACs), targeted to organelle membranes to enable high-resolution imaging of sub-organellar actin dynamics.
@article{Schiavon2020,
  abstract = {The actin cytoskeleton plays multiple critical roles in cells, from cell migration to organelle dynamics. The small and transient actin structures regulating organelle dynamics are challenging to detect with fluorescence microscopy, making it difficult to determine whether actin filaments are directly associated with specific membranes. To address these limitations, we developed fluorescent-protein-tagged actin nanobodies, termed ‘actin chromobodies' (ACs), targeted to organelle membranes to enable high-resolution imaging of sub-organellar actin dynamics.},
  author = {Schiavon, Cara R and Zhang, Tong and Zhao, Bing and Moore, Andrew S and Wales, Pauline and Andrade, Leonardo R and Wu, Melissa and Sung, Tsung-Chang and Dayn, Yelena and Feng, Jasmine W and Quintero, Omar A and Shadel, Gerald S and Grosse, Robert and Manor, Uri},
  doi = {10.1038/s41592-020-0926-5},
  issn = {1548-7105},
  journal = {Nature Methods},
  number = {9},
  pages = {917--921},
  title = {{Actin chromobody imaging reveals sub-organellar actin dynamics}},
  url = {https://doi.org/10.1038/s41592-020-0926-5},
  volume = {17},
  year = {2020}
}

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