Effect of Short- and Long-Term Storage on Human Serum and Recombinant Apolipoprotein E Concentration. Schiele, F., Vincent-Viry, M., Herbeth, B., Visvikis, A., & Siest, G. 38(6):525–528, June, 2000. Publisher: De Gruyter Section: Clinical Chemistry and Laboratory Medicine (CCLM)![Effect of Short- and Long-Term Storage on Human Serum and Recombinant Apolipoprotein E Concentration [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex In order to assess the short- and long-term stability of apolipoprotein (apo) E concentration in serum, we compared the apo E concentrations measured in fresh human serum samples with those determined after storage at +4°C, −20°C or −80°C. The serum apo E concentration was measured by immunoturbidimetry using an anti-human apo E polyclonal antibody from goats. One week storage at +4°C did not significantly affect the serum apo E concentration. At −20°C or −80°C no significant change in apo E concentration occurred during up to three months of storage. Moreover, the concentration of apo E was not modified after long-term storage of serum samples kept at −196°C in liquid nitrogen for up to four years. In addition, 15 freeze-thaw cycles, over a 3-week period, did not affect the apo E concentration in serum. A similar freezethaw procedure applied to purified human recombinant apo E showed that apo E2 isoform was the most stable in comparison with the apo E3 and apo E4 isoforms.
@article{schiele_effect_2000,
title = {Effect of {Short}- and {Long}-{Term} {Storage} on {Human} {Serum} and {Recombinant} {Apolipoprotein} {E} {Concentration}},
volume = {38},
url = {https://www.degruyter.com/document/doi/10.1515/CCLM.2000.077/html},
doi = {10.1515/CCLM.2000.077},
abstract = {In order to assess the short- and long-term stability of apolipoprotein (apo) E concentration in serum, we compared the apo E concentrations measured in fresh human serum samples with those determined after storage at +4°C, −20°C or −80°C. The serum apo E concentration was measured by immunoturbidimetry using an anti-human apo E polyclonal antibody from goats. One week storage at +4°C did not significantly affect the serum apo E concentration. At −20°C or −80°C no significant change in apo E concentration occurred during up to three months of storage. Moreover, the concentration of apo E was not modified after long-term storage of serum samples kept at −196°C in liquid nitrogen for up to four years. In addition, 15 freeze-thaw cycles, over a 3-week period, did not affect the apo E concentration in serum. A similar freezethaw procedure applied to purified human recombinant apo E showed that apo E2 isoform was the most stable in comparison with the apo E3 and apo E4 isoforms.},
language = {en},
number = {6},
urldate = {2023-03-15},
author = {Schiele, Françoise and Vincent-Viry, Monique and Herbeth, Bernard and Visvikis, Athanase and Siest, Gérard},
month = jun,
year = {2000},
note = {Publisher: De Gruyter
Section: Clinical Chemistry and Laboratory Medicine (CCLM)},
pages = {525--528},
}
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