@article{schinkel_small_2001,
	title = {A small family of novel {CuZn}-superoxide dismutases with high isoelectric points in hybrid aspen},
	volume = {213},
	issn = {1432-2048},
	url = {https://doi.org/10.1007/s004250000505},
	doi = {10/fbp32g},
	abstract = {Several CuZn-superoxide dismutases (SODs; EC 1.15.1.1) were cloned from hybrid aspen (Populus tremula L. × tremuloides Michx.). Two of the cloned genes encode representatives of a novel type of CuZn-SOD and we named it HipI-SOD because of its high isoelectric point (≥9). The SODs were cloned by screening a cDNA library with a probe based on a Scots pine (Pinus sylvestris L.) CuZn-SOD that is predominantly located extracellularly. The expression pattern of HipI-SOD was examined using a Northern blot technique and compared with the expression patterns of cytosolic and chloroplastic SODs. Distinct expression patterns were observed for the three types of CuZn-SOD, with HipI-SODs showing strong expression in apical tissues. Southern blots as well as protein analysis suggest that these novel HipI-SODs belong to a small gene family, one member of which might be monomeric.},
	language = {en},
	number = {2},
	urldate = {2021-11-02},
	journal = {Planta},
	author = {Schinkel, Helga and Hertzberg, Magnus and Wingsle, Gunnar},
	month = jun,
	year = {2001},
	pages = {272--279},
}

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