Targeting neural circuitry in zebrafish using GAL4 enhancer trapping. Scott, E. K, Mason, L., Arrenberg, A. B, Ziv, L., Gosse, N. J, Xiao, T., Chi, N. C, Asakawa, K., Kawakami, K., & Baier, H. Nature methods, 4(4):323–6, April, 2007. ![Targeting neural circuitry in zebrafish using GAL4 enhancer trapping. [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex We present a pilot enhancer trap screen using GAL4 to drive expression of upstream activator sequence (UAS)-linked transgenes in expression patterns dictated by endogenous enhancers in zebrafish. The patterns presented include expression in small subsets of neurons throughout the larval brain, which in some cases persist into adult. Through targeted photoconversion of UAS-driven Kaede and variegated expression of UAS-driven GFP in single cells, we begin to characterize the cellular components of labeled circuits.
@article{Scott2007,
title = {Targeting neural circuitry in zebrafish using {GAL4} enhancer trapping.},
volume = {4},
issn = {1548-7091},
url = {http://www.ncbi.nlm.nih.gov/pubmed/17369834},
doi = {10.1038/nmeth1033},
abstract = {We present a pilot enhancer trap screen using GAL4 to drive expression of upstream activator sequence (UAS)-linked transgenes in expression patterns dictated by endogenous enhancers in zebrafish. The patterns presented include expression in small subsets of neurons throughout the larval brain, which in some cases persist into adult. Through targeted photoconversion of UAS-driven Kaede and variegated expression of UAS-driven GFP in single cells, we begin to characterize the cellular components of labeled circuits.},
number = {4},
urldate = {2014-04-04},
journal = {Nature methods},
author = {Scott, Ethan K and Mason, Lindsay and Arrenberg, Aristides B and Ziv, Limor and Gosse, Nathan J and Xiao, Tong and Chi, Neil C and Asakawa, Kazuhide and Kawakami, Koichi and Baier, Herwig},
month = apr,
year = {2007},
pmid = {17369834},
keywords = {Animals, Animals, Genetically Modified, Brain, Brain: cytology, Brain: embryology, Brain: metabolism, DNA-Binding Proteins, Enhancer Elements, Genetic, Gene Expression Regulation, Developmental, Humans, Neurons, Neurons: metabolism, Pilot Projects, Saccharomyces cerevisiae Proteins, Saccharomyces cerevisiae Proteins: biosynthesis, Saccharomyces cerevisiae Proteins: genetics, Transcription Factors, Transcription Factors: biosynthesis, Transcription Factors: genetics, Transgenes, Zebrafish, Zebrafish: embryology, Zebrafish: genetics, Zebrafish: metabolism, enhancer trap},
pages = {323--6},
}
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