Single-Cell Mass Cytometry Analysis of Human Tonsil T Cell Remodeling by Varicella Zoster Virus. Sen, N., Mukherjee, G., Sen, A., Bendall, S., Sung, P., Nolan, G., & Arvin, A. Cell Reports, 8(2):633–645, July, 2014. ![Single-Cell Mass Cytometry Analysis of Human Tonsil T Cell Remodeling by Varicella Zoster Virus [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Summary Although pathogens must infect differentiated host cells that exhibit substantial diversity, documenting the consequences of infection against this heterogeneity is challenging. Single-cell mass cytometry permits deep profiling based on combinatorial expression of surface and intracellular proteins. We used this method to investigate varicella-zoster virus (VZV) infection of tonsil T cells, which mediate viral transport to skin. Our results indicate that VZV induces a continuum of changes regardless of basal phenotypic and functional T cell characteristics. Contrary to the premise that VZV selectively infects T cells with skin trafficking profiles, VZV infection altered T cell surface proteins to enhance or induce these properties. Zap70 and Akt signaling pathways that trigger such surface changes were activated in VZV-infected naive and memory cells by a T cell receptor (TCR)-independent process. Single-cell mass cytometry is likely to be broadly relevant for demonstrating how intracellular pathogens modulate differentiated cells to support pathogenesis in the natural host.
@article{sen_single-cell_2014,
title = {Single-{Cell} {Mass} {Cytometry} {Analysis} of {Human} {Tonsil} {T} {Cell} {Remodeling} by {Varicella} {Zoster} {Virus}},
volume = {8},
issn = {2211-1247},
url = {http://www.sciencedirect.com/science/article/pii/S2211124714004938},
doi = {10.1016/j.celrep.2014.06.024},
abstract = {Summary
Although pathogens must infect differentiated host cells that exhibit substantial diversity, documenting the consequences of infection against this heterogeneity is challenging. Single-cell mass cytometry permits deep profiling based on combinatorial expression of surface and intracellular proteins. We used this method to investigate varicella-zoster virus (VZV) infection of tonsil T~cells, which mediate viral transport to skin. Our results indicate that VZV induces a continuum of changes regardless of basal phenotypic and functional T~cell characteristics. Contrary to the premise that VZV selectively infects T~cells with skin trafficking profiles, VZV infection altered T~cell surface proteins to enhance or induce these properties. Zap70 and Akt signaling pathways that trigger such surface changes were activated in VZV-infected naive and memory cells by a T~cell receptor (TCR)-independent process. Single-cell mass cytometry is likely to be broadly relevant for demonstrating how intracellular pathogens modulate differentiated cells to support pathogenesis in the natural host.},
number = {2},
urldate = {2014-07-29},
journal = {Cell Reports},
author = {Sen, Nandini and Mukherjee, Gourab and Sen, Adrish and Bendall, Sean~C. and Sung, Phillip and Nolan, Garry~P. and Arvin, Ann~M.},
month = jul,
year = {2014},
pages = {633--645}
}
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