@article{serpooshan_bioacoustic-enabled_2017,
	title = {Bioacoustic-enabled patterning of human {iPSC}-derived cardiomyocytes into 3D cardiac tissue.},
	volume = {131},
	url = {https://www.ncbi.nlm.nih.gov/pubmed/28376365},
	doi = {10.1016/j.biomaterials.2017.03.037},
	abstract = {The creation of physiologically-relevant human cardiac tissue with defined cell structure and function is essential for a wide variety of therapeutic, diagnostic, and drug screening applications. Here we report a new scalable method using Faraday waves to enable rapid aggregation of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) into predefined 3D constructs. At packing densities that approximate native myocardium (108-109 cells/ml), these hiPSC-CM-derived 3D tissues demonstrate significantly improved cell viability, metabolic activity, and intercellular connection when compared to constructs with random cell distribution. Moreover, the patterned hiPSC-CMs within the constructs exhibit significantly greater levels of contractile stress, beat frequency, and contraction-relaxation rates, suggesting their improved maturation. Our results demonstrate a novel application of Faraday waves to create stem cell-derived 3D cardiac tissue that resembles the cellular architecture of a native heart tissue for diverse basic research and clinical applications.},
	language = {eng},
	journal = {Biomaterials},
	author = {Serpooshan, V and Chen, P and Wu, H and Lee, S and Sharma, A and Hu, DA and Venkatraman, S and Ganesan, AV and Usta, OB and Yarmush, M and Yang, F and Wu, JC and Demirci, U and Wu, SM},
	year = {2017},
	keywords = {Tissue Engineering},
	pages = {47--57}
}

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Here we report a new scalable method using Faraday waves to enable rapid aggregation of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) into predefined 3D constructs. At packing densities that approximate native myocardium (108-109 cells/ml), these hiPSC-CM-derived 3D tissues demonstrate significantly improved cell viability, metabolic activity, and intercellular connection when compared to constructs with random cell distribution. Moreover, the patterned hiPSC-CMs within the constructs exhibit significantly greater levels of contractile stress, beat frequency, and contraction-relaxation rates, suggesting their improved maturation. Our results demonstrate a novel application of Faraday waves to create stem cell-derived 3D cardiac tissue that resembles the cellular architecture of a native heart tissue for diverse basic research and clinical applications.","language":"eng","journal":"Biomaterials","author":[{"propositions":[],"lastnames":["Serpooshan"],"firstnames":["V"],"suffixes":[]},{"propositions":[],"lastnames":["Chen"],"firstnames":["P"],"suffixes":[]},{"propositions":[],"lastnames":["Wu"],"firstnames":["H"],"suffixes":[]},{"propositions":[],"lastnames":["Lee"],"firstnames":["S"],"suffixes":[]},{"propositions":[],"lastnames":["Sharma"],"firstnames":["A"],"suffixes":[]},{"propositions":[],"lastnames":["Hu"],"firstnames":["DA"],"suffixes":[]},{"propositions":[],"lastnames":["Venkatraman"],"firstnames":["S"],"suffixes":[]},{"propositions":[],"lastnames":["Ganesan"],"firstnames":["AV"],"suffixes":[]},{"propositions":[],"lastnames":["Usta"],"firstnames":["OB"],"suffixes":[]},{"propositions":[],"lastnames":["Yarmush"],"firstnames":["M"],"suffixes":[]},{"propositions":[],"lastnames":["Yang"],"firstnames":["F"],"suffixes":[]},{"propositions":[],"lastnames":["Wu"],"firstnames":["JC"],"suffixes":[]},{"propositions":[],"lastnames":["Demirci"],"firstnames":["U"],"suffixes":[]},{"propositions":[],"lastnames":["Wu"],"firstnames":["SM"],"suffixes":[]}],"year":"2017","keywords":"Tissue Engineering","pages":"47–57","bibtex":"@article{serpooshan_bioacoustic-enabled_2017,\n\ttitle = {Bioacoustic-enabled patterning of human {iPSC}-derived cardiomyocytes into 3D cardiac tissue.},\n\tvolume = {131},\n\turl = {https://www.ncbi.nlm.nih.gov/pubmed/28376365},\n\tdoi = {10.1016/j.biomaterials.2017.03.037},\n\tabstract = {The creation of physiologically-relevant human cardiac tissue with defined cell structure and function is essential for a wide variety of therapeutic, diagnostic, and drug screening applications. Here we report a new scalable method using Faraday waves to enable rapid aggregation of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) into predefined 3D constructs. At packing densities that approximate native myocardium (108-109 cells/ml), these hiPSC-CM-derived 3D tissues demonstrate significantly improved cell viability, metabolic activity, and intercellular connection when compared to constructs with random cell distribution. Moreover, the patterned hiPSC-CMs within the constructs exhibit significantly greater levels of contractile stress, beat frequency, and contraction-relaxation rates, suggesting their improved maturation. 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