{"_id":"tsiPC2JAspuEFg6Es","bibbaseid":"shafiee-caldwell-sano-davalos-contactlessdielectrophoresisanewtechniqueforcellmanipulation-2009","author_short":["Shafiee, H.","Caldwell, J. L.","Sano, M. B.","Davalos, R. V."],"bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["Shafiee"],"firstnames":["H."],"suffixes":[]},{"propositions":[],"lastnames":["Caldwell"],"firstnames":["J.","L."],"suffixes":[]},{"propositions":[],"lastnames":["Sano"],"firstnames":["M.","B."],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["R.","V."],"suffixes":[]}],"title":"Contactless dielectrophoresis: a new technique for cell manipulation","journal":"Biomed Microdevices","volume":"11","number":"5","pages":"997-1006","note":"1572-8781 Shafiee, Hadi Caldwell, John L Sano, Michael B Davalos, Rafael V Journal Article Research Support, Non-U.S. Gov't United States 2009/05/06 Biomed Microdevices. 2009 Oct;11(5):997-1006. doi: 10.1007/s10544-009-9317-5. Epub 2009 May 5.","abstract":"Dielectrophoresis (DEP) has become a promising technique to separate and identify cells and microparticles suspended in a medium based on their size or electrical properties. Presented herein is a new technique to provide the non-uniform electric field required for DEP that does not require electrodes to contact the sample fluid. In our method, electrodes are capacitively-coupled to a fluidic channel through dielectric barriers; the application of a high-frequency electric field to these electrodes then induces an electric field in the channel. This technique combines the cell manipulation abilities of traditional DEP with the ease of fabrication found in insulator-based technologies. A microfluidic device was fabricated based on this principle to determine the feasibility of cell manipulations through contactless DEP (cDEP). We were able to demonstrate cell responses unique to the DEP effect in three separate cell lines. These results illustrate the potential for this technique to identify cells through their electrical properties without fear of contamination from electrodes.","keywords":"Cell Line, Tumor Cell Separation/*instrumentation Dimethylpolysiloxanes/chemistry Electric Impedance Electrodes Electrophoresis/*instrumentation Humans Microfluidic Analytical Techniques/*instrumentation Models, Theoretical Rotation","issn":"1387-2176","doi":"10.1007/s10544-009-9317-5","year":"2009","bibtex":"@article{RN238,\n author = {Shafiee, H. and Caldwell, J. L. and Sano, M. B. and Davalos, R. V.},\n title = {Contactless dielectrophoresis: a new technique for cell manipulation},\n journal = {Biomed Microdevices},\n volume = {11},\n number = {5},\n pages = {997-1006},\n note = {1572-8781\nShafiee, Hadi\nCaldwell, John L\nSano, Michael B\nDavalos, Rafael V\nJournal Article\nResearch Support, Non-U.S. Gov't\nUnited States\n2009/05/06\nBiomed Microdevices. 2009 Oct;11(5):997-1006. doi: 10.1007/s10544-009-9317-5. Epub 2009 May 5.},\n abstract = {Dielectrophoresis (DEP) has become a promising technique to separate and identify cells and microparticles suspended in a medium based on their size or electrical properties. Presented herein is a new technique to provide the non-uniform electric field required for DEP that does not require electrodes to contact the sample fluid. In our method, electrodes are capacitively-coupled to a fluidic channel through dielectric barriers; the application of a high-frequency electric field to these electrodes then induces an electric field in the channel. This technique combines the cell manipulation abilities of traditional DEP with the ease of fabrication found in insulator-based technologies. A microfluidic device was fabricated based on this principle to determine the feasibility of cell manipulations through contactless DEP (cDEP). We were able to demonstrate cell responses unique to the DEP effect in three separate cell lines. These results illustrate the potential for this technique to identify cells through their electrical properties without fear of contamination from electrodes.},\n keywords = {Cell Line, Tumor\nCell Separation/*instrumentation\nDimethylpolysiloxanes/chemistry\nElectric Impedance\nElectrodes\nElectrophoresis/*instrumentation\nHumans\nMicrofluidic Analytical Techniques/*instrumentation\nModels, Theoretical\nRotation},\n ISSN = {1387-2176},\n DOI = {10.1007/s10544-009-9317-5},\n year = {2009},\n type = {Journal Article}\n}\n\n","author_short":["Shafiee, H.","Caldwell, J. L.","Sano, M. B.","Davalos, R. V."],"key":"RN238","id":"RN238","bibbaseid":"shafiee-caldwell-sano-davalos-contactlessdielectrophoresisanewtechniqueforcellmanipulation-2009","role":"author","urls":{},"keyword":["Cell Line","Tumor Cell Separation/*instrumentation Dimethylpolysiloxanes/chemistry Electric Impedance Electrodes Electrophoresis/*instrumentation Humans Microfluidic Analytical Techniques/*instrumentation Models","Theoretical Rotation"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/network/files/bdNBTZRXTsoHCgpbh","dataSources":["zaL9693Ha8EYPcn6R","FWKuFu88GYvaFfxyp","wnTqRCfBSz2AkAxJo","BWcnpMbDFTWngT8TX","ntzd2BXQzDvcuBAni","ud8ZKuHT43FjZJtFt","oFDkexDhXJBsNbdmC","dwEFKjxyXZxuiMwXp","D4zENc4BfFNBwSYYJ","ZPLjameRikygaiM9B","3XfNmZkLe6o8CvECW","fJQsxtBoqymHQG6tL","LzxgEApraxMPkLTMn","Z2THpXfLYEJf3CB8p"],"keywords":["cell line","tumor cell separation/*instrumentation dimethylpolysiloxanes/chemistry electric impedance electrodes electrophoresis/*instrumentation humans microfluidic analytical techniques/*instrumentation models","theoretical rotation"],"search_terms":["contactless","dielectrophoresis","new","technique","cell","manipulation","shafiee","caldwell","sano","davalos"],"title":"Contactless dielectrophoresis: a new technique for cell manipulation","year":2009}