{"_id":"rTbLcHP7hLfLhWyip","bibbaseid":"shafiee-sano-henslee-caldwell-davalos-selectiveisolationoflivedeadcellsusingcontactlessdielectrophoresiscdep-2010","author_short":["Shafiee, H.","Sano, M. B.","Henslee, E. A.","Caldwell, J. L.","Davalos, R. V."],"bibdata":{"bibtype":"article","type":"Journal Article","author":[{"propositions":[],"lastnames":["Shafiee"],"firstnames":["H."],"suffixes":[]},{"propositions":[],"lastnames":["Sano"],"firstnames":["M.","B."],"suffixes":[]},{"propositions":[],"lastnames":["Henslee"],"firstnames":["E.","A."],"suffixes":[]},{"propositions":[],"lastnames":["Caldwell"],"firstnames":["J.","L."],"suffixes":[]},{"propositions":[],"lastnames":["Davalos"],"firstnames":["R.","V."],"suffixes":[]}],"title":"Selective isolation of live/dead cells using contactless dielectrophoresis (cDEP)","journal":"Lab Chip","volume":"10","number":"4","pages":"438-45","note":"Shafiee, Hadi Sano, Michael B Henslee, Erin A Caldwell, John L Davalos, Rafael V Journal Article Research Support, Non-U.S. Gov't England 2010/02/04 Lab Chip. 2010 Feb 21;10(4):438-45. doi: 10.1039/b920590j. Epub 2010 Jan 19.","abstract":"Contactless dielectrophoresis (cDEP) is a recently developed method of cell manipulation in which the electrodes are physically isolated from the sample. Here we present two microfluidic devices capable of selectively isolating live human leukemia cells from dead cells utilizing their electrical signatures. The effect of different voltages and frequencies on the gradient of the electric field and device performance was investigated numerically and validated experimentally. With these prototype devices we were able to achieve greater than 95% removal efficiency at 0.2-0.5 mm s(-1) with 100% selectivity between live and dead cells. In conjunction with enrichment, cDEP could be integrated with other technologies to yield fully automated lab-on-a-chip systems capable of sensing, sorting, and identifying rare cells.","keywords":"Animals Cell Death Cell Line, Tumor Cell Separation/instrumentation/*methods Cell Survival Electric Conductivity Electrophoresis Humans","issn":"1473-0197 (Print) 1473-0189","doi":"10.1039/b920590j","year":"2010","bibtex":"@article{RN233,\n author = {Shafiee, H. and Sano, M. B. and Henslee, E. A. and Caldwell, J. L. and Davalos, R. V.},\n title = {Selective isolation of live/dead cells using contactless dielectrophoresis (cDEP)},\n journal = {Lab Chip},\n volume = {10},\n number = {4},\n pages = {438-45},\n note = {Shafiee, Hadi\nSano, Michael B\nHenslee, Erin A\nCaldwell, John L\nDavalos, Rafael V\nJournal Article\nResearch Support, Non-U.S. Gov't\nEngland\n2010/02/04\nLab Chip. 2010 Feb 21;10(4):438-45. doi: 10.1039/b920590j. Epub 2010 Jan 19.},\n abstract = {Contactless dielectrophoresis (cDEP) is a recently developed method of cell manipulation in which the electrodes are physically isolated from the sample. Here we present two microfluidic devices capable of selectively isolating live human leukemia cells from dead cells utilizing their electrical signatures. The effect of different voltages and frequencies on the gradient of the electric field and device performance was investigated numerically and validated experimentally. With these prototype devices we were able to achieve greater than 95% removal efficiency at 0.2-0.5 mm s(-1) with 100% selectivity between live and dead cells. In conjunction with enrichment, cDEP could be integrated with other technologies to yield fully automated lab-on-a-chip systems capable of sensing, sorting, and identifying rare cells.},\n keywords = {Animals\nCell Death\nCell Line, Tumor\nCell Separation/instrumentation/*methods\nCell Survival\nElectric Conductivity\nElectrophoresis\nHumans},\n ISSN = {1473-0197 (Print)\n1473-0189},\n DOI = {10.1039/b920590j},\n year = {2010},\n type = {Journal Article}\n}\n\n","author_short":["Shafiee, H.","Sano, M. B.","Henslee, E. A.","Caldwell, J. L.","Davalos, R. V."],"key":"RN233","id":"RN233","bibbaseid":"shafiee-sano-henslee-caldwell-davalos-selectiveisolationoflivedeadcellsusingcontactlessdielectrophoresiscdep-2010","role":"author","urls":{},"keyword":["Animals Cell Death Cell Line","Tumor Cell Separation/instrumentation/*methods Cell Survival Electric Conductivity Electrophoresis Humans"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/network/files/bdNBTZRXTsoHCgpbh","dataSources":["zaL9693Ha8EYPcn6R","FWKuFu88GYvaFfxyp","wnTqRCfBSz2AkAxJo","BWcnpMbDFTWngT8TX","ntzd2BXQzDvcuBAni","ud8ZKuHT43FjZJtFt","oFDkexDhXJBsNbdmC","dwEFKjxyXZxuiMwXp","D4zENc4BfFNBwSYYJ","ZPLjameRikygaiM9B","3XfNmZkLe6o8CvECW","fJQsxtBoqymHQG6tL","LzxgEApraxMPkLTMn","Z2THpXfLYEJf3CB8p"],"keywords":["animals cell death cell line","tumor cell separation/instrumentation/*methods cell survival electric conductivity electrophoresis humans"],"search_terms":["selective","isolation","live","dead","cells","using","contactless","dielectrophoresis","cdep","shafiee","sano","henslee","caldwell","davalos"],"title":"Selective isolation of live/dead cells using contactless dielectrophoresis (cDEP)","year":2010}