Identification of Rad51 protein from Chlamydomonas reinhardtii: Recombinational characteristics. Shalguev, V., Kaboev, O., Sizova, I., Hegemann, P., & Lanzov, V. Molekulyarnaya Biologiya, 39(1):112-119, 2005. cited By 0
Paper abstract bibtex The unicellular green microalga Chlamydomonas reinhardtii is a perspective model object for basic and applied research. However, its homologous recombination (HR) system which lies in the basis of double-strand DNA break repair have still not been studied. Last years the program of C. reinhardtii nuclear genome sequence is realized and different nucleotide repeats in the genome structure have been revealed that can explain a low level of HR relative to nonhomologous recombination events. Analyses of the C. reinhardtii EST (Expressed Sequence Tag)- and genome libraries permitted us to reconstruct and clone cDNA of the RAD51 gene. In present work, the cDNA was expressed, its product was purified and some basal biochemical activities were studied. The results show that Rad51 protein from lower eukaryote C. reinhardtii is identified as typical representative of the Rad51C-like subfamily of higher eukaryotes.
@ARTICLE{Shalguev2005112,
author={Shalguev, V.I. and Kaboev, O.K. and Sizova, I.A. and Hegemann, P. and Lanzov, V.A.},
title={Identification of Rad51 protein from Chlamydomonas reinhardtii: Recombinational characteristics},
journal={Molekulyarnaya Biologiya},
year={2005},
volume={39},
number={1},
pages={112-119},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-21244474443&partnerID=40&md5=709da542bb9b29c04ab3585eaec056f1},
affiliation={Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina/Sankt-Petersburg, 188300, Russian Federation; St. Petersburg State University, Sankt-Petersburg, 198904, Russian Federation; Institute für Biochemie, Genetik, and Microbiologie, Universität Regensburg, Germany},
abstract={The unicellular green microalga Chlamydomonas reinhardtii is a perspective model object for basic and applied research. However, its homologous recombination (HR) system which lies in the basis of double-strand DNA break repair have still not been studied. Last years the program of C. reinhardtii nuclear genome sequence is realized and different nucleotide repeats in the genome structure have been revealed that can explain a low level of HR relative to nonhomologous recombination events. Analyses of the C. reinhardtii EST (Expressed Sequence Tag)- and genome libraries permitted us to reconstruct and clone cDNA of the RAD51 gene. In present work, the cDNA was expressed, its product was purified and some basal biochemical activities were studied. The results show that Rad51 protein from lower eukaryote C. reinhardtii is identified as typical representative of the Rad51C-like subfamily of higher eukaryotes.},
correspondence_address1={Shalguev, V.I.; Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina/Sankt-Petersburg, 188300, Russian Federation},
issn={00268984},
coden={MOBIB},
pubmed_id={15773555},
language={Russian},
abbrev_source_title={Mol. Biol.},
document_type={Article},
source={Scopus},
}
Downloads: 0
{"_id":"u32gEuXhd69udpseL","bibbaseid":"shalguev-kaboev-sizova-hegemann-lanzov-identificationofrad51proteinfromchlamydomonasreinhardtiirecombinationalcharacteristics-2005","author_short":["Shalguev, V.","Kaboev, O.","Sizova, I.","Hegemann, P.","Lanzov, V."],"bibdata":{"bibtype":"article","type":"article","author":[{"propositions":[],"lastnames":["Shalguev"],"firstnames":["V.I."],"suffixes":[]},{"propositions":[],"lastnames":["Kaboev"],"firstnames":["O.K."],"suffixes":[]},{"propositions":[],"lastnames":["Sizova"],"firstnames":["I.A."],"suffixes":[]},{"propositions":[],"lastnames":["Hegemann"],"firstnames":["P."],"suffixes":[]},{"propositions":[],"lastnames":["Lanzov"],"firstnames":["V.A."],"suffixes":[]}],"title":"Identification of Rad51 protein from Chlamydomonas reinhardtii: Recombinational characteristics","journal":"Molekulyarnaya Biologiya","year":"2005","volume":"39","number":"1","pages":"112-119","note":"cited By 0","url":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-21244474443&partnerID=40&md5=709da542bb9b29c04ab3585eaec056f1","affiliation":"Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina/Sankt-Petersburg, 188300, Russian Federation; St. Petersburg State University, Sankt-Petersburg, 198904, Russian Federation; Institute für Biochemie, Genetik, and Microbiologie, Universität Regensburg, Germany","abstract":"The unicellular green microalga Chlamydomonas reinhardtii is a perspective model object for basic and applied research. However, its homologous recombination (HR) system which lies in the basis of double-strand DNA break repair have still not been studied. Last years the program of C. reinhardtii nuclear genome sequence is realized and different nucleotide repeats in the genome structure have been revealed that can explain a low level of HR relative to nonhomologous recombination events. Analyses of the C. reinhardtii EST (Expressed Sequence Tag)- and genome libraries permitted us to reconstruct and clone cDNA of the RAD51 gene. In present work, the cDNA was expressed, its product was purified and some basal biochemical activities were studied. The results show that Rad51 protein from lower eukaryote C. reinhardtii is identified as typical representative of the Rad51C-like subfamily of higher eukaryotes.","correspondence_address1":"Shalguev, V.I.; Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina/Sankt-Petersburg, 188300, Russian Federation","issn":"00268984","coden":"MOBIB","pubmed_id":"15773555","language":"Russian","abbrev_source_title":"Mol. Biol.","document_type":"Article","source":"Scopus","bibtex":"@ARTICLE{Shalguev2005112,\r\nauthor={Shalguev, V.I. and Kaboev, O.K. and Sizova, I.A. and Hegemann, P. and Lanzov, V.A.},\r\ntitle={Identification of Rad51 protein from Chlamydomonas reinhardtii: Recombinational characteristics},\r\njournal={Molekulyarnaya Biologiya},\r\nyear={2005},\r\nvolume={39},\r\nnumber={1},\r\npages={112-119},\r\nnote={cited By 0},\r\nurl={https://www.scopus.com/inward/record.uri?eid=2-s2.0-21244474443&partnerID=40&md5=709da542bb9b29c04ab3585eaec056f1},\r\naffiliation={Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina/Sankt-Petersburg, 188300, Russian Federation; St. Petersburg State University, Sankt-Petersburg, 198904, Russian Federation; Institute für Biochemie, Genetik, and Microbiologie, Universität Regensburg, Germany},\r\nabstract={The unicellular green microalga Chlamydomonas reinhardtii is a perspective model object for basic and applied research. However, its homologous recombination (HR) system which lies in the basis of double-strand DNA break repair have still not been studied. Last years the program of C. reinhardtii nuclear genome sequence is realized and different nucleotide repeats in the genome structure have been revealed that can explain a low level of HR relative to nonhomologous recombination events. Analyses of the C. reinhardtii EST (Expressed Sequence Tag)- and genome libraries permitted us to reconstruct and clone cDNA of the RAD51 gene. In present work, the cDNA was expressed, its product was purified and some basal biochemical activities were studied. The results show that Rad51 protein from lower eukaryote C. reinhardtii is identified as typical representative of the Rad51C-like subfamily of higher eukaryotes.},\r\ncorrespondence_address1={Shalguev, V.I.; Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina/Sankt-Petersburg, 188300, Russian Federation},\r\nissn={00268984},\r\ncoden={MOBIB},\r\npubmed_id={15773555},\r\nlanguage={Russian},\r\nabbrev_source_title={Mol. Biol.},\r\ndocument_type={Article},\r\nsource={Scopus},\r\n}\r\n\r\n","author_short":["Shalguev, V.","Kaboev, O.","Sizova, I.","Hegemann, P.","Lanzov, V."],"key":"Shalguev2005112","id":"Shalguev2005112","bibbaseid":"shalguev-kaboev-sizova-hegemann-lanzov-identificationofrad51proteinfromchlamydomonasreinhardtiirecombinationalcharacteristics-2005","role":"author","urls":{"Paper":"https://www.scopus.com/inward/record.uri?eid=2-s2.0-21244474443&partnerID=40&md5=709da542bb9b29c04ab3585eaec056f1"},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bio.pnpi.nrcki.ru/wp-content/uploads/2019/12/lmkb_2019_10.txt","dataSources":["rgjRz8LhiuL9Prc4S","gJDkC9aHANwJxgtHb"],"keywords":[],"search_terms":["identification","rad51","protein","chlamydomonas","reinhardtii","recombinational","characteristics","shalguev","kaboev","sizova","hegemann","lanzov"],"title":"Identification of Rad51 protein from Chlamydomonas reinhardtii: Recombinational characteristics","year":2005}