Minimum information for reporting on the TEER (trans-epithelial/endothelial electrical resistance) assay (MIRTA). Sharma, M., Huber, E., Arnesdotter, E., Behrsing, H. P., Bettmann, A., Brandwein, D., Constant, S., Date, R., Deshpande, A., Fabian, E., Gupta, A., Gutierrez, R., Gutleb, A. C., Hargrove, M. M., Hollings, M., Hutter, V., Jarabek, A. M., Kaluzhny, Y., Landsiedel, R., Milchak, L., Moyer, R. A., Murray, J. R., Page, K., Patel, M., Pearson, S. N., Petersen, E. J., Reinke, E., Roldan, N., Roper, C., Scaglione, J. B., Settivari, R. S., Stucki, A. O., Verstraelen, S., Wallace, J. L., McCullough, S., & Clippinger, A. J. Archives of Toxicology, 99(1):57–66, January, 2025.
Minimum information for reporting on the TEER (trans-epithelial/endothelial electrical resistance) assay (MIRTA) [link]Paper  doi  abstract   bibtex   
Standard information reporting helps to ensure that assay conditions and data are consistently reported and to facilitate inter-laboratory comparisons. Here, we present recommendations on minimum information for reporting on the TEER (trans-epithelial/endothelial electrical resistance) assay (MIRTA). The TEER assay is extensively used to evaluate the health of an epithelial/endothelial cell culture model and as an indicator of the potential toxicity of a test substance. This publication is the result of an international collaboration─called the RespTox (Respiratory Toxicity) Collaborative─through which twelve laboratories shared their protocols for assessing the barrier function of respiratory epithelial cells using the TEER assay following exposure to substances. The protocols from each laboratory were reviewed to identify general steps for performing the TEER assay, interlaboratory differences between steps, the rationale for differences, whether these differences impact results or cross-laboratory comparisons between TEER measurements. While the MIRTA recommendations are focused on respiratory epithelial cell systems, these recommendations can be adapted for other cell systems that form barriers. The use of these recommendations will support data transparency and reproducibility, reduce challenges in data interpretation, enable cross-laboratory comparisons, help assess study quality, and facilitate the incorporation of the TEER assay into national and international testing guidance.
@article{sharma_minimum_2025,
	title = {Minimum information for reporting on the {TEER} (trans-epithelial/endothelial electrical resistance) assay ({MIRTA})},
	volume = {99},
	issn = {1432-0738},
	url = {https://doi.org/10.1007/s00204-024-03879-z},
	doi = {10.1007/s00204-024-03879-z},
	abstract = {Standard information reporting helps to ensure that assay conditions and data are consistently reported and to facilitate inter-laboratory comparisons. Here, we present recommendations on minimum information for reporting on the TEER (trans-epithelial/endothelial electrical resistance) assay (MIRTA). The TEER assay is extensively used to evaluate the health of an epithelial/endothelial cell culture model and as an indicator of the potential toxicity of a test substance. This publication is the result of an international collaboration─called the RespTox (Respiratory Toxicity) Collaborative─through which twelve laboratories shared their protocols for assessing the barrier function of respiratory epithelial cells using the TEER assay following exposure to substances. The protocols from each laboratory were reviewed to identify general steps for performing the TEER assay, interlaboratory differences between steps, the rationale for differences, whether these differences impact results or cross-laboratory comparisons between TEER measurements. While the MIRTA recommendations are focused on respiratory epithelial cell systems, these recommendations can be adapted for other cell systems that form barriers. The use of these recommendations will support data transparency and reproducibility, reduce challenges in data interpretation, enable cross-laboratory comparisons, help assess study quality, and facilitate the incorporation of the TEER assay into national and international testing guidance.},
	language = {en},
	number = {1},
	urldate = {2025-07-14},
	journal = {Archives of Toxicology},
	author = {Sharma, Monita and Huber, Erin and Arnesdotter, Emma and Behrsing, Holger P. and Bettmann, Adam and Brandwein, David and Constant, Samuel and Date, Rahul and Deshpande, Abhay and Fabian, Eric and Gupta, Amit and Gutierrez, Robert and Gutleb, Arno C. and Hargrove, Marie M. and Hollings, Michael and Hutter, Victoria and Jarabek, Annie M. and Kaluzhny, Yulia and Landsiedel, Robert and Milchak, Lawrence and Moyer, Robert A. and Murray, Jessica R. and Page, Kathryn and Patel, Manish and Pearson, Stephanie N. and Petersen, Elijah J. and Reinke, Emily and Roldan, Nuria and Roper, Clive and Scaglione, Jamie B. and Settivari, Raja S. and Stucki, Andreas O. and Verstraelen, Sandra and Wallace, Joanne L. and McCullough, Shaun and Clippinger, Amy J.},
	month = jan,
	year = {2025},
	keywords = {Barrier integrity, Biomarkers, Flow cytometry, In vitro, Inhalation, Respiratory toxicity, Reverse transcription polymerase chain reaction, Scanning Transmission Electron Microscopy, TEER, Transmission Electron Microscopy, miRNAs},
	pages = {57--66},
}
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