Folding thermodynamics of the hybrid-1 type intramolecular human telomeric G-quadruplex. Shek, Y. L., Noudeh, G. D., Nazari, M., Heerklotz, H., Abu-Ghazalah, R. M, Dubins, D. N, & Chalikian, T. V Biopolymers, 101(3):216–27, March, 2014. ![Folding thermodynamics of the hybrid-1 type intramolecular human telomeric G-quadruplex. [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Guanine-rich DNA sequences that may form G-quadruplexes are located in strategic DNA loci with the ability to regulate biological events. G-quadruplexes have been under intensive scrutiny owing to their potential to serve as novel drug targets in emerging anticancer strategies. Thermodynamic characterization of G-quadruplexes is an important and necessary step in developing predictive algorithms for evaluating the conformational preferences of G-rich sequences in the presence or the absence of their complementary C-rich strands. We use a combination of spectroscopic, calorimetric, and volumetric techniques to characterize the folding/unfolding transitions of the 26-meric human telomeric sequence d[A3 G3 (T2 AG3 )3 A2 ]. In the presence of K(+) ions, the latter adopts the hybrid-1 G-quadruplex conformation, a tightly packed structure with an unusually small number of solvent-exposed atomic groups. The K(+) -induced folding of the G-quadruplex at room temperature is a slow process that involves significant accumulation of an intermediate at the early stages of the transition. The G-quadruplex state of the oligomeric sequence is characterized by a larger volume and compressibility and a smaller expansibility than the coil state. These results are in qualitative agreement with each other all suggesting significant dehydration to accompany the G-quadruplex formation. Based on our volume data, 432 ± 19 water molecules become released to the bulk upon the G-quadruplex formation. This large number is consistent with a picture in which DNA dehydration is not limited to water molecules in direct contact with the regions that become buried but involves a general decrease in solute-solvent interactions all over the surface of the folded structure. © 2013 Wiley Periodicals, Inc. Biopolymers 101: 216-227, 2014.
@article{Shek2014,
title = {Folding thermodynamics of the hybrid-1 type intramolecular human telomeric {G}-quadruplex.},
volume = {101},
issn = {0006-3525},
url = {http://www.ncbi.nlm.nih.gov/pubmed/23775839},
doi = {10.1002/bip.22317},
abstract = {Guanine-rich DNA sequences that may form G-quadruplexes are located in strategic DNA loci with the ability to regulate biological events. G-quadruplexes have been under intensive scrutiny owing to their potential to serve as novel drug targets in emerging anticancer strategies. Thermodynamic characterization of G-quadruplexes is an important and necessary step in developing predictive algorithms for evaluating the conformational preferences of G-rich sequences in the presence or the absence of their complementary C-rich strands. We use a combination of spectroscopic, calorimetric, and volumetric techniques to characterize the folding/unfolding transitions of the 26-meric human telomeric sequence d[A3 G3 (T2 AG3 )3 A2 ]. In the presence of K(+) ions, the latter adopts the hybrid-1 G-quadruplex conformation, a tightly packed structure with an unusually small number of solvent-exposed atomic groups. The K(+) -induced folding of the G-quadruplex at room temperature is a slow process that involves significant accumulation of an intermediate at the early stages of the transition. The G-quadruplex state of the oligomeric sequence is characterized by a larger volume and compressibility and a smaller expansibility than the coil state. These results are in qualitative agreement with each other all suggesting significant dehydration to accompany the G-quadruplex formation. Based on our volume data, 432 ± 19 water molecules become released to the bulk upon the G-quadruplex formation. This large number is consistent with a picture in which DNA dehydration is not limited to water molecules in direct contact with the regions that become buried but involves a general decrease in solute-solvent interactions all over the surface of the folded structure. © 2013 Wiley Periodicals, Inc. Biopolymers 101: 216-227, 2014.},
number = {3},
journal = {Biopolymers},
author = {Shek, Yuen Lai and Noudeh, Golamreza Dehghan and Nazari, Mozhgan and Heerklotz, Heiko and Abu-Ghazalah, Rashid M and Dubins, David N and Chalikian, Tigran V},
month = mar,
year = {2014},
pmid = {23775839},
keywords = {\#nosource, accepted pre-, compressibility, conformational transitions, date corresponds to the, expansibility, g-quadruplexes, preprint, print, published online, published online as an, the, this article was originally, volume},
pages = {216--27},
}
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Thermodynamic characterization of G-quadruplexes is an important and necessary step in developing predictive algorithms for evaluating the conformational preferences of G-rich sequences in the presence or the absence of their complementary C-rich strands. We use a combination of spectroscopic, calorimetric, and volumetric techniques to characterize the folding/unfolding transitions of the 26-meric human telomeric sequence d[A3 G3 (T2 AG3 )3 A2 ]. In the presence of K(+) ions, the latter adopts the hybrid-1 G-quadruplex conformation, a tightly packed structure with an unusually small number of solvent-exposed atomic groups. The K(+) -induced folding of the G-quadruplex at room temperature is a slow process that involves significant accumulation of an intermediate at the early stages of the transition. The G-quadruplex state of the oligomeric sequence is characterized by a larger volume and compressibility and a smaller expansibility than the coil state. 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