Measurements of Oxygen Evolution in Photosynthesis. Shevela, D., Schröder, W. P., & Messinger, J. In Photosynthesis : Methods and Protocols, pages 133–148. Springer US, New York, NY, 2024. Paper doi abstract bibtex This chapter compares two different techniques for monitoring photosynthetic O2 production; the wide-spread Clark-type O2 electrode and the more sophisticated membrane inlet mass spectrometry (MIMS) technique. We describe how a simple membrane inlet for MIMS can be made out of a commercial Clark-type cell and outline the advantages and drawbacks of the two techniques to guide researchers in deciding which method to use. Protocols and examples are given for measuring O2 evolution rates and for determining the number of chlorophyll molecules per active photosystem II reaction center.
@incollection{shevela_measurements_2024,
address = {New York, NY},
title = {Measurements of {Oxygen} {Evolution} in {Photosynthesis}},
isbn = {978-1-07-163790-6},
url = {https://doi.org/10.1007/978-1-0716-3790-6_8},
abstract = {This chapter compares two different techniques for monitoring photosynthetic O2 production; the wide-spread Clark-type O2 electrode and the more sophisticated membrane inlet mass spectrometry (MIMS) technique. We describe how a simple membrane inlet for MIMS can be made out of a commercial Clark-type cell and outline the advantages and drawbacks of the two techniques to guide researchers in deciding which method to use. Protocols and examples are given for measuring O2 evolution rates and for determining the number of chlorophyll molecules per active photosystem II reaction center.},
language = {en},
urldate = {2024-10-16},
booktitle = {Photosynthesis : {Methods} and {Protocols}},
publisher = {Springer US},
author = {Shevela, Dmitry and Schröder, Wolfgang P. and Messinger, Johannes},
editor = {Covshoff, Sarah},
year = {2024},
doi = {10.1007/978-1-0716-3790-6_8},
keywords = {Clark-type electrode, Membrane-inlet mass spectrometry, O2 evolution, Oxygenic photosynthesis, Photosynthetic water oxidation, Photosynthetic water splitting, Photosystem II},
pages = {133--148},
}
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