Granzyme B Functionalized Nanoparticles Targeting Membrane Hsp70-Positive Tumors for Multimodal Cancer Theranostics. Shevtsov, M., Stangl, S., Nikolaev, B., Yakovleva, L., Marchenko, Y., Tagaeva, R., Sievert, W., Pitkin, E., Mazur, A., Tolstoy, P., Galibin, O., Ryzhov, V., Steiger, K., Smirnov, O., Khachatryan, W., Chester, K., & Multhoff, G. Small, 2019. cited By 0![Granzyme B Functionalized Nanoparticles Targeting Membrane Hsp70-Positive Tumors for Multimodal Cancer Theranostics [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Functionalized superparamagnetic iron oxide nanoparticles (SPIONs) have emerged as potential clinical tools for cancer theranostics. Membrane-bound 70 kDa heat shock protein (mHsp70) is ubiquitously expressed on the cell membrane of various tumor types but not normal cells and therefore provides a tumor-specific target. The serine protease granzyme B (GrB) that is produced as an effector molecule by activated T and NK cells has been shown to specifically target mHsp70 on tumor cells. Following binding to Hsp70, GrB is rapidly internalized into tumor cells. Herein, it is demonstrated that GrB functionalized SPIONs act as a contrast enhancement agent for magnetic resonance imaging and induce specific tumor cell apoptosis. Combinatorial regimens employing stereotactic radiotherapy and/or magnetic targeting are found to further enhance the therapeutic efficacy of GrB-SPIONs in different tumor mouse models. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
@ARTICLE{Shevtsov2019,
author={Shevtsov, M. and Stangl, S. and Nikolaev, B. and Yakovleva, L. and Marchenko, Y. and Tagaeva, R. and Sievert, W. and Pitkin, E. and Mazur, A. and Tolstoy, P. and Galibin, O. and Ryzhov, V. and Steiger, K. and Smirnov, O. and Khachatryan, W. and Chester, K. and Multhoff, G.},
title={Granzyme B Functionalized Nanoparticles Targeting Membrane Hsp70-Positive Tumors for Multimodal Cancer Theranostics},
journal={Small},
year={2019},
volume={15},
number={13},
doi={10.1002/smll.201900205},
art_number={1900205},
note={cited By 0},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062364915&doi=10.1002%2fsmll.201900205&partnerID=40&md5=94d7b54710320cc39d17d9efcd89854f},
affiliation={Center for Translational Cancer Research Technische Universität München (TranslaTUM), Radiation Immuno-Oncology group, Klinikum rechts der Isar, Einsteinstr. 25, Munich, 81675, Germany; Institute of Cytology of the Russian Academy of Sciences (RAS), Tikhoretsky ave., 4, St. Petersburg, 194064, Russian Federation; First Pavlov State Medical University of St. Petersburg, L'va Tolstogo str. 6/8, St. Petersburg, 197022, Russian Federation; Almazov National Medical Research Centre, Russian Polenov Neurosurgical Institute, Mayakovskogo str. 12, St. Petersburg, 191104, Russian Federation; Research Institute of Highly Pure Biopreparations, Pudozhskaya str. 12, St. Petersburg, 191014, Russian Federation; Wharton School, University of Pennsylvania, Walnut Street 3730, Philadelphia, PA 19104, United States; Saint Petersburg State University, Universitetskaya nab. 7-9, St. Petersburg, 199034, Russian Federation; NRC “Kurchatov Institute”, Petersburg Nuclear Physics Institute, Gatchina, 188300, Russian Federation; Institute of Pathology, Technische Universität München, Trogerstr. 18, Munich, 81675, Germany; UCL Cancer Institute, University College London, 72 Huntley Street, London, WC1E 6DD, United Kingdom},
abstract={Functionalized superparamagnetic iron oxide nanoparticles (SPIONs) have emerged as potential clinical tools for cancer theranostics. Membrane-bound 70 kDa heat shock protein (mHsp70) is ubiquitously expressed on the cell membrane of various tumor types but not normal cells and therefore provides a tumor-specific target. The serine protease granzyme B (GrB) that is produced as an effector molecule by activated T and NK cells has been shown to specifically target mHsp70 on tumor cells. Following binding to Hsp70, GrB is rapidly internalized into tumor cells. Herein, it is demonstrated that GrB functionalized SPIONs act as a contrast enhancement agent for magnetic resonance imaging and induce specific tumor cell apoptosis. Combinatorial regimens employing stereotactic radiotherapy and/or magnetic targeting are found to further enhance the therapeutic efficacy of GrB-SPIONs in different tumor mouse models. © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim},
author_keywords={glioblastoma; granzyme B; magnetic targeting; radiotherapy; superparamagnetic nanoparticles},
funding_details={Deutsche ForschungsgemeinschaftSTA1520/1-1, SFB824/3},
funding_details={Bundesministerium für Bildung und Forschung01GU0823, 02NUK038A0},
funding_details={Allianz Industrie ForschungZF4320102CS7},
funding_details={Российский Фонд Фундаментальных Исследований (РФФИ)19-08-00024},
}
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