Tracking Calcium Dynamics and Immune Surveillance at the Choroid Plexus Blood-Cerebrospinal Fluid Interface. Shipley, F. B., Dani, N., Xu, H., Deister, C., Cui, J., Head, J. P., Sadegh, C., Fame, R. M., Shannon, M. L., Flores, V. I., Kishkovich, T., Jang, E., Klein, E. M., Goldey, G. J., He, K., Zhang, Y., Holtzman, M. J., Kirchhausen, T., Wyart, C., Moore, C. I., Andermann, M. L., & Lehtinen, M. K. Neuron, September, 2020. Place: United States
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The choroid plexus (ChP) epithelium is a source of secreted signaling factors in cerebrospinal fluid (CSF) and a key barrier between blood and brain. Here, we develop imaging tools to interrogate these functions in adult lateral ventricle ChP in whole-mount explants and in awake mice. By imaging epithelial cells in intact ChP explants, we observed calcium activity and secretory events that increased in frequency following delivery of serotonergic agonists. Using chronic two-photon imaging in awake mice, we observed spontaneous subcellular calcium events as well as strong agonist-evoked calcium activation and cytoplasmic secretion into CSF. Three-dimensional imaging of motility and mobility of multiple types of ChP immune cells at baseline and following immune challenge or focal injury revealed a range of surveillance and defensive behaviors. Together, these tools should help illuminate the diverse functions of this understudied body-brain interface.
@article {shipley_tracking_2020,
	title = {Tracking {Calcium} {Dynamics} and {Immune} {Surveillance} at the {Choroid} {Plexus}  {Blood}-{Cerebrospinal} {Fluid} {Interface}.},
	copyright = {Copyright © 2020 Elsevier Inc. All rights reserved.},
	issn = {1097-4199 0896-6273},
	doi = {10.1016/j.neuron.2020.08.024},
	abstract = {The choroid plexus (ChP) epithelium is a source of secreted signaling factors in  cerebrospinal fluid (CSF) and a key barrier between blood and brain. Here, we  develop imaging tools to interrogate these functions in adult lateral ventricle ChP  in whole-mount explants and in awake mice. By imaging epithelial cells in intact ChP  explants, we observed calcium activity and secretory events that increased in  frequency following delivery of serotonergic agonists. Using chronic two-photon  imaging in awake mice, we observed spontaneous subcellular calcium events as well as  strong agonist-evoked calcium activation and cytoplasmic secretion into CSF.  Three-dimensional imaging of motility and mobility of multiple types of ChP immune  cells at baseline and following immune challenge or focal injury revealed a range of  surveillance and defensive behaviors. Together, these tools should help illuminate  the diverse functions of this understudied body-brain interface.},
	language = {eng},
	journal = {Neuron},
	author = {Shipley, Frederick B. and Dani, Neil and Xu, Huixin and Deister, Christopher and Cui, Jin and Head, Joshua P. and Sadegh, Cameron and Fame, Ryann M. and Shannon, Morgan L. and Flores, Vanessa I. and Kishkovich, Thomas and Jang, Emily and Klein, Eric M. and Goldey, Glenn J. and He, Kangmin and Zhang, Yong and Holtzman, Michael J. and Kirchhausen, Tomas and Wyart, Claire and Moore, Christopher I. and Andermann, Mark L. and Lehtinen, Maria K.},
	month = sep,
	year = {2020},
	pmid = {32961128},
	note = {Place: United States},
	keywords = {calcium activity, cerebrospinal fluid, choroid plexus, epithelial cells, immune cells, secretion, serotonin, two-photon imaging}
}

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