Aggregation by lectins as an approach for exosome isolation from biological fluids: Validation for proteomic studies. Shtam, T., Burdakov, V., Landa, S., Naryzhny, S., Bairamukov, V., Malek, A., Orlov, Y., & Filatov, M. Cell and Tissue Biology, 11(2):172-179, Maik Nauka-Interperiodica Publishing, 2017. cited By 4![Aggregation by lectins as an approach for exosome isolation from biological fluids: Validation for proteomic studies [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Exosomes, a special type of microparticles produced by cells, are currently of considerable interest for researchers. The term “exosomes” denotes extracellular vesicles of less than 120 nm in size derived from intracellular multivesicular bodies. Multiple studies that address the distinctive features of exosome structure and biochemical composition in various pathological states imply the possibility of development of novel diagnostic techniques based on the detection of changes in the pool of proteins and nucleic acids transported by exosomes. However, methods for isolation and investigation of exosomes are rather difficult to develop because of a small size of these vesicles. A novel approach for preparative-scale isolation of exosomes based on the phenomenon of binding and aggregation of these particles in the presence of lectins has been put forward in the present study. The method developed is relatively cost-effective, allows for the isolation of exosomes from various biological fluids, and has been validated for the subsequent analysis of the protein composition of the exosomes in view of the possible clinical applications. The validation showed that the sedimentation of lectin-aggregated exosomes is a suitable approach for the isolation of these microvesicles from the complete conditioned culture medium in a research-laboratory setup. © 2017, Pleiades Publishing, Ltd.
@ARTICLE{Shtam2017172,
author={Shtam, T.A. and Burdakov, V.S. and Landa, S.B. and Naryzhny, S.N. and Bairamukov, V.Y. and Malek, A.V. and Orlov, Y.N. and Filatov, M.V.},
title={Aggregation by lectins as an approach for exosome isolation from biological fluids: Validation for proteomic studies},
journal={Cell and Tissue Biology},
year={2017},
volume={11},
number={2},
pages={172-179},
doi={10.1134/S1990519X17020043},
note={cited By 4},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018499633&doi=10.1134%2fS1990519X17020043&partnerID=40&md5=f439cee2262ea4552aa9c299648a59e1},
affiliation={National Research Centre “Kurchatov Institute” B.P. Konstantinov Petersburg Nuclear Physics Institute, Gatchina, 188300, Russian Federation; Peter the Great Polytechnic University, St. Petersburg, 195251, Russian Federation; Petrov Institute of Oncology, Ministry of Healthcare of the Russian Federation, St. Petersburg, 197758, Russian Federation; Orekhovich Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, 119121, Russian Federation},
abstract={Exosomes, a special type of microparticles produced by cells, are currently of considerable interest for researchers. The term “exosomes” denotes extracellular vesicles of less than 120 nm in size derived from intracellular multivesicular bodies. Multiple studies that address the distinctive features of exosome structure and biochemical composition in various pathological states imply the possibility of development of novel diagnostic techniques based on the detection of changes in the pool of proteins and nucleic acids transported by exosomes. However, methods for isolation and investigation of exosomes are rather difficult to develop because of a small size of these vesicles. A novel approach for preparative-scale isolation of exosomes based on the phenomenon of binding and aggregation of these particles in the presence of lectins has been put forward in the present study. The method developed is relatively cost-effective, allows for the isolation of exosomes from various biological fluids, and has been validated for the subsequent analysis of the protein composition of the exosomes in view of the possible clinical applications. The validation showed that the sedimentation of lectin-aggregated exosomes is a suitable approach for the isolation of these microvesicles from the complete conditioned culture medium in a research-laboratory setup. © 2017, Pleiades Publishing, Ltd.},
author_keywords={exosomes; lectins; methods of exosome isolation},
correspondence_address1={Filatov, M.V.; National Research Centre “Kurchatov Institute” B.P. Konstantinov Petersburg Nuclear Physics InstituteRussian Federation; email: fil_53@mail.ru},
publisher={Maik Nauka-Interperiodica Publishing},
issn={1990519X},
language={English},
abbrev_source_title={Cell Tissue Biol.},
document_type={Article},
source={Scopus},
}
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