Molecular architecture of photosynthetic membranes in Rhodobacter sphaeroides: the role of PufX. Siebert, C. A., Qian, P., Fotiadis, D., Engel, A., Hunter, C. N., & Bullough, P. A. The EMBO Journal, 23(4):690--700, February, 2004.
Molecular architecture of photosynthetic membranes in Rhodobacter sphaeroides: the role of PufX [link]Paper  doi  abstract   bibtex   
The effects of the PufX polypeptide on membrane architecture were investigated by comparing the composition and structures of photosynthetic membranes from PufX+ and PufX- strains of Rhodobacter sphaeroides. We show that this single polypeptide profoundly affects membrane morphology, leading to highly elongated cells containing extended tubular membranes. Purified tubular membranes contain helical arrays composed solely of dimeric RC–LH1–PufX (RC, reaction centre; LH, light harvesting) complexes with apparently open LH1 rings. PufX- cells contain crystalline membranes with a pseudo-hexagonal packing of monomeric core complexes. Analysis of purified complexes by electron microscopy and atomic force microscopy shows that LH1 and PufX form a continuous ring of protein around each RC. A model of the tubular membrane is presented with PufX located adjacent to the stained region created by a vacant LH1. This arrangement, coupled with a flexible ring, would give the RC QB site transient access to the interstices in the lattice, which might be of functional importance. We discuss the implications of our data for the export of quinol from the RC, for eventual reduction of the cytochrome bc1 complex.
@article{siebert_molecular_2004,
	title = {Molecular architecture of photosynthetic membranes in {Rhodobacter} sphaeroides: the role of {PufX}},
	volume = {23},
	copyright = {© 2004 Nature Publishing Group},
	issn = {0261-4189},
	shorttitle = {Molecular architecture of photosynthetic membranes in {Rhodobacter} sphaeroides},
	url = {http://www.nature.com/emboj/journal/v23/n4/full/7600092a.html},
	doi = {10.1038/sj.emboj.7600092},
	abstract = {The effects of the PufX polypeptide on membrane architecture were investigated by comparing the composition and structures of photosynthetic membranes from PufX+ and PufX- strains of Rhodobacter sphaeroides. We show that this single polypeptide profoundly affects membrane morphology, leading to highly elongated cells containing extended tubular membranes. Purified tubular membranes contain helical arrays composed solely of dimeric RC–LH1–PufX (RC, reaction centre; LH, light harvesting) complexes with apparently open LH1 rings. PufX- cells contain crystalline membranes with a pseudo-hexagonal packing of monomeric core complexes. Analysis of purified complexes by electron microscopy and atomic force microscopy shows that LH1 and PufX form a continuous ring of protein around each RC. A model of the tubular membrane is presented with PufX located adjacent to the stained region created by a vacant LH1. This arrangement, coupled with a flexible ring, would give the RC QB site transient access to the interstices in the lattice, which might be of functional importance. We discuss the implications of our data for the export of quinol from the RC, for eventual reduction of the cytochrome bc1 complex.},
	language = {en},
	number = {4},
	urldate = {2013-03-16TZ},
	journal = {The EMBO Journal},
	author = {Siebert, C. Alistair and Qian, Pu and Fotiadis, Dimitrios and Engel, Andreas and Hunter, C. Neil and Bullough, Per A.},
	month = feb,
	year = {2004},
	keywords = {2D crystals, Photosynthesis, cytochrome bc1 complex, light harvesting complex, reaction centre},
	pages = {690--700}
}

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