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@article{
 title = {Classic zinc finger from friend of GATA mediates an interaction with the coiled-coil of transforming acidic coiled-coil 3},
 type = {article},
 year = {2004},
 identifiers = {[object Object]},
 pages = {39789-39797},
 volume = {279},
 websites = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-4544366535&doi=10.1074%2Fjbc.M404130200&partnerID=40&md5=0f131222a132dcba70daed2a4487b34a},
 id = {5c9d3a6d-094f-32b9-82b5-a8e3f79b3a58},
 created = {2020-10-01T06:20:29.182Z},
 file_attached = {false},
 profile_id = {45e2f77d-7a48-31e0-99de-4a07a154b051},
 group_id = {c2dc1a38-fc29-3f20-8d91-e4e1a13d85ae},
 last_modified = {2020-10-01T06:20:29.182Z},
 read = {false},
 starred = {false},
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 source_type = {ARTICLE},
 notes = {cited By 26},
 private_publication = {false},
 abstract = {Classic zinc finger domains (cZFs) consist of a β-hairpin followed by an α-helix. They are among the most abundant of all protein domains and are often found in tandem arrays in DNA-binding proteins, with each finger contributing an α-helix to effect sequence-specific DNA recognition. Lone cZFs, not found in tandem arrays, have been postulated to function in protein interactions. We have studied the transcriptional co-regulator Friend of GATA (FOG), which contains nine zinc fingers. We have discovered that the third cZF of FOG contacts a coiled-coil domain in the centrosomal protein transforming acidic coiled-coil 3 (TACC3). Although FOG-ZF3 exhibited low solubility, we have used a combination of mutational mapping and protein engineering to generate a derivative that was suitable for in vitro and structural analysis. We report that the α-helix of FOG-ZF3 recognizes a C-terminal portion of the TACC3 coiled-coil. Remarkably, the α-helical surface utilized by FOG-ZF3 is the same surface responsible for the well established sequence-specific DNA-binding properties of many other cZFs. Our data demonstrate the versatility of cZFs and have implications for the analysis of many as yet uncharacterized cZF proteins.},
 bibtype = {article},
 author = {Simpson, Raina J.Y. and Lee, Stella Hoi Yi and Bartle, Natalie and Sum, Eleanor Y. and Visvader, Jane E. and Matthews, Jacqueline M. and Mackay, Joel P. and Crossley, Merlin},
 journal = {Journal of Biological Chemistry},
 number = {38}
}