T/B cell interactions are more transient in response to weak stimuli in SLE-prone mice. Sinai, P., Dozmorov, I. M., Song, R., Schwartzberg, P. L., Wakeland, E. K., & Wülfing, C. European journal of immunology, 44(12):3522–3531, December, 2014. ![T/B cell interactions are more transient in response to weak stimuli in SLE-prone mice [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Changes in immune function during the course of systemic lupus erythematosus (SLE) are well characterized. Class-switched antinuclear antibodies are the hallmark of SLE, and T/B- cell interactions are thus critical. However, changes in immune function contributing to disease susceptibility are unknown. Here, we have analyzed primary T and B cells from a mouse model of SLE prior to the onset of disease. To allow cognate T-cell activation with low affinity, we have developed a lower potency peptide ligand for the OTII T-cell receptor. T and B-cell couples formed less frequently and retained their polarity less efficiently preferentially in response to low affinity stimulation in SLE-prone mice. This matched decreased recruitment of actin and Vav1 and an enhanced PKCΘ recruitment to the cellular interface in T cells. The induction of the germinal center B-cell marker GL7 was increased in T/B cell couples from SLE-prone mice when the T-cell numbers were limited. However, the overall gene expression changes were marginal. Taken together, the enhanced cell couple transience may allow a more efficient sampling of a large number of T/B cell couples, preferentially in response to limiting stimuli, therefore enhancing the immune reactivity in the development of SLE.
@article{sinai_tb_2014,
title = {T/{B} cell interactions are more transient in response to weak stimuli in {SLE}-prone mice},
volume = {44},
issn = {0014-2980},
url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4261040/},
doi = {10.1002/eji.201444602},
abstract = {Changes in immune function during the course of systemic lupus erythematosus (SLE) are well characterized. Class-switched antinuclear antibodies are the hallmark of SLE, and T/B- cell interactions are thus critical. However, changes in immune function contributing to disease susceptibility are unknown. Here, we have analyzed primary T and B cells from a mouse model of SLE prior to the onset of disease. To allow cognate T-cell activation with low affinity, we have developed a lower potency peptide ligand for the OTII T-cell receptor. T and B-cell couples formed less frequently and retained their polarity less efficiently preferentially in response to low affinity stimulation in SLE-prone mice. This matched decreased recruitment of actin and Vav1 and an enhanced PKCΘ recruitment to the cellular interface in T cells. The induction of the germinal center B-cell marker GL7 was increased in T/B cell couples from SLE-prone mice when the T-cell numbers were limited. However, the overall gene expression changes were marginal. Taken together, the enhanced cell couple transience may allow a more efficient sampling of a large number of T/B cell couples, preferentially in response to limiting stimuli, therefore enhancing the immune reactivity in the development of SLE.},
number = {12},
urldate = {2021-04-14},
journal = {European journal of immunology},
author = {Sinai, Parisa and Dozmorov, Igor M. and Song, Ran and Schwartzberg, Pamela L. and Wakeland, Edward K. and Wülfing, Christoph},
month = dec,
year = {2014},
pmid = {25209945},
pmcid = {PMC4261040},
pages = {3522--3531},
}
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To allow cognate T-cell activation with low affinity, we have developed a lower potency peptide ligand for the OTII T-cell receptor. T and B-cell couples formed less frequently and retained their polarity less efficiently preferentially in response to low affinity stimulation in SLE-prone mice. This matched decreased recruitment of actin and Vav1 and an enhanced PKCΘ recruitment to the cellular interface in T cells. The induction of the germinal center B-cell marker GL7 was increased in T/B cell couples from SLE-prone mice when the T-cell numbers were limited. However, the overall gene expression changes were marginal. 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